SlVQ15 recruits SlWRKY30IIc to link with jasmonate pathway in regulating tomato defence against root-knot nematodes.

IF 10.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Plant Biotechnology Journal Pub Date : 2024-11-05 DOI:10.1111/pbi.14493
Huang Huang, Xuechun Ma, Lulu Sun, Yingying Wang, Jilin Ma, Yihan Hong, Mingjie Zhao, Wenchao Zhao, Rui Yang, Susheng Song, Shaohui Wang
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Abstract

Tomato is one of the most economically important vegetable crops in the world and has been seriously affected by the devastating agricultural pest root-knot nematodes (RKNs). Current understanding of tomato resistance to RKNs is quite limited. VQ motif-containing family proteins are plant-specific regulators; however, whether and how tomato VQs regulate resistance to RKNs is unknown. Here, we found that SlVQ15 recruited SlWRKY30IIc to coordinately control tomato defence against the RKN Meloidogyne incognita without affecting plant growth and productivity. The jasmonate (JA)-ZIM domain (JAZ) repressors of the phytohormone JAs signalling associated and interfered with the interaction of SlVQ15 and SlWRKY30IIc. In turn, SlWRKY30IIc bound to SlJAZs promoters and cooperated with SlVQ15 to repress their expression, whereas this inhibitory effect was antagonized by SlJAZ5, forming a feedback regulatory mechanism. Moreover, SlWRKY30IIc expression was directly regulated by SlMYC2, a SlJAZ-interacting negative regulator of resistance to RKNs. In conclusion, our findings revealed that a regulatory circuit of SlVQ15-SlWRKY30IIc and the JA pathway fine-tunes tomato defence against the RKN M. incognita, and provided candidate genes and clues with great potential for crop improvement.

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SlVQ15招募SlWRKY30IIc与茉莉酸盐途径连接,调节番茄对根结线虫的防御。
番茄是世界上经济价值最高的蔬菜作物之一,受到毁灭性农业害虫根结线虫(RKNs)的严重影响。目前对番茄抗根结线虫能力的了解还很有限。含 VQ 矩阵的家族蛋白是植物特异性调节因子,但番茄的 VQ 是否以及如何调节对 RKNs 的抗性尚不清楚。在这里,我们发现 SlVQ15 招募 SlWRKY30IIc 来协调控制番茄对 RKN Meloidogyne incognita 的防御,而不会影响植物的生长和产量。植物激素 JA 信号的茉莉酸(JA)-ZIM 结构域(JAZ)抑制因子与 SlVQ15 和 SlWRKY30IIc 的相互作用相关联并对其产生干扰。反过来,SlWRKY30IIc 与 SlJAZs 启动子结合,并与 SlVQ15 合作抑制其表达,而这种抑制作用被 SlJAZ5 所拮抗,形成一种反馈调控机制。此外,SlWRKY30IIc的表达受SlMYC2的直接调控,SlMYC2是与SlJAZ相互作用的抗RKNs的负调控因子。总之,我们的研究结果表明,SlVQ15-SlWRKY30IIc和JA通路的调控回路可微调番茄对RKN M. incognita的抗性,并为作物改良提供了候选基因和线索,具有巨大的潜力。
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来源期刊
Plant Biotechnology Journal
Plant Biotechnology Journal 生物-生物工程与应用微生物
CiteScore
20.50
自引率
2.90%
发文量
201
审稿时长
1 months
期刊介绍: Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.
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