Dendritic mesoporous silica loaded with gold nanoclusters and their application in immunochromatographic assay

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2024-11-05 DOI:10.1007/s00604-024-06808-0

Tao Peng, Zhanwei Liang, Jihao Si, Lu Huang, Hua Ye, Xiaoqing Li, Miao Sun, Jie Xie, Xinhua Dai

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Abstract

Novel green-emitting fluorescent microspheres (GreFMPs) were assembled by loading highly luminescent gold nanoclusters (Arg/ATT/AuNCs) on dendritic mesoporous silica nanoparticles (DMSNs) via PEI-mediated electrostatic adsorption. The fluorescence microspheres exhibit excellent monodispersion with average diameters about (254.5 ± 34.6 nm). Compared with free Arg/ATT/AuNCs, the GreFMPs have similar fluorescent properties and biocompatibility but superior environmental tolerance. Subsequently, an immunochromatographic assay based on GreFMPs (GreFMP-ICA) has been successfully developed, which is highly selective toward alpha fetoprotein (AFP) in human serum. Under the optimal parameters, there is a good linear range between 0.5 and 160.0 ng/mL, the limit of detection was found to be about 0.5 ng/mL, and the recovery and relative standard deviation are 81.0 ~ 100.6% and 3.5 ~ 16.6%, respectively. These results manifested that the GreFMPs are beneficial for the rational design of the ICA platform, and the proposed GreFMP-ICA has the potential to screen AFP in clinical applications.

Graphical Abstract

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负载金纳米团簇的树枝状介孔二氧化硅及其在免疫层析检测中的应用。

通过 PEI 介导的静电吸附，将高发光金纳米团簇（Arg/ATT/AuNCs）负载在树枝状介孔二氧化硅纳米颗粒（DMSNs）上，组装成新型绿色发光荧光微球（GreFMPs）。荧光微球具有良好的单分散性，平均直径约为（254.5 ± 34.6 nm）。与游离 Arg/ATT/AuNCs 相比，GreFMPs 具有相似的荧光特性和生物相容性，但环境耐受性更好。随后，一种基于 GreFMPs 的免疫层析检测方法（GreFMP-ICA）被成功开发出来，它对人血清中的甲胎蛋白（AFP）具有高度选择性。在最佳参数下，其线性范围为 0.5 至 160.0 ng/mL，检出限约为 0.5 ng/mL，回收率和相对标准偏差分别为 81.0% 至 100.6%，3.5% 至 16.6%。这些结果表明，GreFMPs有利于ICA平台的合理设计，所提出的GreFMP-ICA具有在临床应用中筛选AFP的潜力。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.