Biosynthesis-Based Spatial Metabolome of Condensed Tannin Reveals Its Role in Salt Tolerance of Non-Salt-Secretor Mangrove Kandelia obovata.

IF 6 1区 生物学 Q1 PLANT SCIENCES Plant, Cell & Environment Pub Date : 2024-11-06 DOI:10.1111/pce.15269
Hezi Huang, Lihan Zhuang, Hanchen Tang, Zhaoyu Guo, Qinghua Li, Zejin Lin, Mingjin Dai, Xiuxiu Wang, Yifan Wang, Hailei Zheng, Xueyi Zhu
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Abstract

An autofluorescent inclusion (AFI) specifically accumulated in mesophyll cells (MCs) of non-salt-secretor mangrove was found to be related to salt, but its biosynthesis and spatial distribution characteristics remain unclear. Here, Kandelia obovata served as the experimental material, and the composition of AFI was identified as condensed tannin (CT). Na contents increased in purified AFIs under NaCl treatment, while Na+ efflux in MCs was lower than the control. In vitro, Na+ addition caused aggregations of AFIs. Proteins related to Na+/H+ and vesicle transport were identified in the purified AFIs by liquid chromatography-mass spectrometry. TEM images revealed the structures involved in CT biosynthesis in chloroplasts and CT accretions in vacuoles were more visible under higher salinity. Spatial metabolomics analysis on flavonoid metabolites involving in CT biosynthesis illustrated those flavonoids and three CT monomers were positively related to salt in MCs. Real-time quantitative PCR verified the genes encoding enzymes for CT biosynthesis were upregulated accordingly. Taken together, CT biosynthesis is positively correlated with Na accumulation in leaves. The CTs synthesized in chloroplasts are transported as shuttles to vacuole via cytoplasm, facilitating the sequestration and compartmentalization of excessive Na+ ions into the vacuole, which confers non-salt-secretor mangrove K. obovata a higher salt tolerance.

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基于生物合成的缩合单宁空间代谢组揭示了其在非盐遁红树林 Kandelia obovata 的耐盐性中的作用。
研究发现,非盐分泌型红树林叶肉细胞(MC)中特异性积累的自发荧光包涵体(AFI)与盐有关,但其生物合成和空间分布特征仍不清楚。本文以 Kandelia obovata 为实验材料,确定 AFI 的成分为缩合单宁(CT)。在NaCl处理下,纯化的AFIs中Na含量增加,而MCs中Na+外流低于对照组。在体外,Na+的添加会导致AFIs聚集。液相色谱-质谱法鉴定了纯化的AFIs中与Na+/H+和囊泡运输相关的蛋白质。TEM 图像显示了叶绿体中参与 CT 生物合成的结构,在盐度较高的情况下,液泡中的 CT 增生更为明显。对参与 CT 生物合成的类黄酮代谢物的空间代谢组学分析表明,类黄酮和三种 CT 单体与 MCs 中的盐分呈正相关。实时定量 PCR 验证了编码 CT 生物合成酶的基因相应上调。综上所述,CT 的生物合成与叶片中 Na 的积累呈正相关。叶绿体中合成的 CT 通过细胞质以梭子的形式运输到液泡中,促进了过量 Na+ 离子在液泡中的封存和分隔,从而使不分泌盐分的红树林 K. obovata 具有更高的耐盐性。
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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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