Simultaneous assessment of cerebral glucose and oxygen metabolism and perfusion in rats using interleaved deuterium (²H) and oxygen-17 (¹⁷O) MRS.

IF 2.7 4区医学 Q2 BIOPHYSICS NMR in Biomedicine Pub Date : 2024-11-06 DOI:10.1002/nbm.5284

Guangle Zhang, Parker Jenkins, Wei Zhu, Wei Chen, Xiao-Hong Zhu

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Abstract

Cerebral glucose and oxygen metabolism and blood perfusion play key roles in neuroenergetics and oxidative phosphorylation to produce adenosine triphosphate (ATP) energy molecules in supporting cellular activity and brain function. Their impairments have been linked to numerous brain disorders. This study aimed to develop an in vivo magnetic resonance spectroscopy (MRS) method capable of simultaneously assessing and quantifying the major cerebral metabolic rates of glucose (CMR_Glc) and oxygen (CMRO₂) consumption, lactate formation (CMR_Lac), and tricarboxylic acid (TCA) cycle (V_TCA); cerebral blood flow (CBF); and oxygen extraction fraction (OEF) via a single dynamic MRS measurement using an interleaved deuterium (²H) and oxygen-17 (¹⁷O) MRS approach. We introduced a single-loop multifrequency radio-frequency (RF) surface coil that can be used to acquire proton (¹H) magnetic resonance imaging (MRI) or interleaved low-γ X-nuclei ²H and ¹⁷O MRS. By combining this RF coil with a modified MRS pulse sequence, ¹⁷O-isotope-labeled oxygen gas inhalation, and intravenous ²H-isotope-labeled glucose administration, we demonstrate for the first time the feasibility of simultaneously and quantitatively measuring six important physiological parameters, CMR_Glc, CMRO₂, CMR_Lac, V_TCA, CBF, and OEF, in rat brains at 16.4 T. The interleaved ²H-¹⁷O MRS technique should be readily adapted to image and study cerebral energy metabolism and perfusion in healthy and diseased brains.

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利用交错氘（2H）和氧-17（17O）磁共振成像（MRS）同时评估大鼠的脑葡萄糖和氧代谢及灌注。

脑葡萄糖和氧代谢以及血液灌注在神经能量和氧化磷酸化过程中发挥着关键作用，以产生三磷酸腺苷（ATP）能量分子，支持细胞活动和大脑功能。它们的损伤与许多脑部疾病有关。本研究旨在开发一种体内磁共振光谱（MRS）方法，能够同时评估和量化葡萄糖（CMRGlc）和氧（CMRO2）消耗、乳酸形成（CMRLac）和三羧酸（TCA）循环（VTCA）等主要脑代谢率；我们采用交错氘（2H）和氧-17（17O）MRS 方法，通过单次动态 MRS 测量获得了脑血流量（CBF）和氧萃取分数（OEF）。我们引入了一种单回路多频射频（RF）表面线圈，可用于获取质子（1H）磁共振成像（MRI）或交错低γ X核 2H和17O MRS。通过将这种射频线圈与改进的 MRS 脉冲序列、17O-同位素标记的氧气吸入和静脉注射 2H-同位素标记的葡萄糖相结合，我们首次证明了在 16.4 T 下同时定量测量大鼠大脑中 CMRGlc、CMRO2、CMRLac、VTCA、CBF 和 OEF 这六个重要生理参数的可行性。交错 2H-17O MRS 技术应能很容易地用于健康和患病大脑能量代谢和灌注的成像和研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

NMR in Biomedicine 医学-光谱学

CiteScore

6.00

自引率

10.30%

发文量

209

审稿时长

3-8 weeks

期刊介绍： NMR in Biomedicine is a journal devoted to the publication of original full-length papers, rapid communications and review articles describing the development of magnetic resonance spectroscopy or imaging methods or their use to investigate physiological, biochemical, biophysical or medical problems. Topics for submitted papers should be in one of the following general categories: (a) development of methods and instrumentation for MR of biological systems; (b) studies of normal or diseased organs, tissues or cells; (c) diagnosis or treatment of disease. Reports may cover work on patients or healthy human subjects, in vivo animal experiments, studies of isolated organs or cultured cells, analysis of tissue extracts, NMR theory, experimental techniques, or instrumentation.