Evaluation of the QIAstat-Dx BCID GN and GPF kits for direct identification and antimicrobial resistance prediction from blood culture bottles.

IF 6.1 2区 医学 Q1 MICROBIOLOGY Journal of Clinical Microbiology Pub Date : 2024-11-06 DOI:10.1128/jcm.01169-24
Sally Ng, Chai Yuin Tan, Jing Yan Yah, Nur A'tikah Binte Osman, Ka Lip Chew
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Abstract

Rapid multiplex PCR kits have been used for rapid identification of blood culture isolates and prediction of antimicrobial resistance. We performed an evaluation of the QIAstat-Dx BCID GN and GPF research use only (RUO) kits on positive blood culture bottles using routine laboratory testing as the reference standard. Positive blood culture bottles between November 2023 and January 2024 were tested with QIAstat-Dx BCID GN and GPF kits based on initial Gram stain results and compared against routine identification and phenotypic susceptibility testing. A total of 174 monomicrobial blood cultures were included in the final analysis. The 174 monomicrobial blood cultures composed of 129 BCID GN tests and 45 BCID GPF tests. The majority of on-target Gram-negative organisms in monomicrobial cultures were identified. One Escherichia coli isolate was not identified as such, although the pan-Enterobacterales target was positive. All on-target Gram-positive organisms in monomicrobial cultures were identified. Overall sensitivity and specificity of tem/shv for detection of aminopenicillin resistance in E. coli was 94.7% (18/19) and 95.8% (23/24). The presence/absence of ctx-m and ampC had 100% sensitivity and specificity for identification of third-generation cephalosporin resistance in E. coli and Klebsiella pneumoniae. The combination of blaZ and mecA gene detection was fully predictive of phenotypic susceptibility results to penicillin and cloxacillin for Staphylococcus aureus. Overall, the QIAstat-Dx BCID GN and GPF kits were able to identify on-target pathogens. Detected resistance mechanisms were highly predictive of β-lactam resistance. Prediction of resistance for non-β-lactam antimicrobial was more variable.

Importance: This is one of the first evaluations of the QIAstat BCID kit and demonstrates high levels of correlation for both identification and antimicrobial resistance prediction.

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评估 QIAstat-Dx BCID GN 和 GPF 检测试剂盒对血培养瓶的直接鉴定和抗菌药耐药性预测。
快速多重 PCR 检测试剂盒已被用于快速鉴定血培养分离物和预测抗菌药耐药性。我们以常规实验室检测为参考标准,在阳性血培养瓶上对 QIAstat-Dx BCID GN 和 GPF 仅供研究使用 (RUO) 的试剂盒进行了评估。根据最初的革兰氏染色结果,用 QIAstat-Dx BCID GN 和 GPF 试剂盒对 2023 年 11 月至 2024 年 1 月期间的阳性血培养瓶进行了检测,并与常规鉴定和表型药敏试验进行了比较。共有 174 份单微生物血液培养物被纳入最终分析。这 174 份单微生物血液培养包括 129 次 BCID GN 检测和 45 次 BCID GPF 检测。在单微生物培养物中鉴定出了大多数目标革兰氏阴性菌。有一个大肠埃希氏菌分离物未被鉴定为大肠埃希氏菌,尽管泛肠道菌目标呈阳性。在单微生物培养物中鉴定出了所有目标革兰氏阳性微生物。tem/shv检测大肠杆菌对氨青霉素耐药性的总体灵敏度和特异性分别为94.7%(18/19)和95.8%(23/24)。ctx-m和ampC对鉴定大肠埃希菌和肺炎克雷伯菌对第三代头孢菌素耐药的敏感性和特异性均为100%。结合检测 blaZ 和 mecA 基因完全可以预测金黄色葡萄球菌对青霉素和氯唑西林的表型药敏结果。总体而言,QIAstat-Dx BCID GN 和 GPF 试剂盒能够鉴定目标病原体。检测到的耐药性机制对β-内酰胺类耐药性有很高的预测性。对非β-内酰胺类抗菌药物耐药性的预测则变化较大:重要意义:这是对 QIAstat BCID 试剂盒进行的首次评估之一,结果表明该试剂盒在鉴定和抗菌药耐药性预测方面都具有很高的相关性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Clinical Microbiology
Journal of Clinical Microbiology 医学-微生物学
CiteScore
17.10
自引率
4.30%
发文量
347
审稿时长
3 months
期刊介绍: The Journal of Clinical Microbiology® disseminates the latest research concerning the laboratory diagnosis of human and animal infections, along with the laboratory's role in epidemiology and the management of infectious diseases.
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