ATF3 mediates PM2.5-induced apoptosis and inflammation in ovarian granulosa cells.

IF 3.8 3区 医学 Q1 REPRODUCTIVE BIOLOGY Journal of Ovarian Research Pub Date : 2024-11-05 DOI:10.1186/s13048-024-01539-5
Xiandan Zhang, Xuan Wang, Hao Li, Haihong Wang, Dewei Du, Huijuan Huang
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Abstract

Particulate matter 2.5 (PM2.5) pollution has emerged as a major global public health concern because of its adverse effects on human health. Our group has previously demonstrated that PM2.5 exposure can seriously impair ovarian function. However, the underlying mechanisms remain a mystery. This study verifies ovarian damage in mice, evidenced by inflammatory cell infiltration and follicular atresia, following 5 months of PM2.5 exposure via tracheal drip (35 µg/m³ for low dose and 150 µg/m³ for high dose). In addition, PM2.5 exposure inhibited the cell viability of human granulosa cells (KGN) and induced apoptosis at the concentrations of 50, 100, and 150 µg/mL for 24 h. The apoptosis of KGN cells induced by inflammation contributes to follicular atresia. Furthermore, we conducted RNA-sequencing analysis to identify the genes and pathways triggered by PM2.5 (100 µg/mL) exposure, which decreases the KGN cell viability. We found a significant increase in Activating Transcription Factor 3 (ATF3). Further mechanistic studies reveal a strong association between PM2.5-induced apoptosis, inflammation, and ATF3 with its downstream oxidative stress signals. In summary, the ATF3 pathway serves a vital role in the ovarian injury caused by PM2.5 exposures.

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ATF3 介导 PM2.5 诱导的卵巢颗粒细胞凋亡和炎症。
颗粒物 2.5(PM2.5)污染因其对人类健康的不利影响,已成为全球公共卫生关注的一个主要问题。我们的研究小组先前已经证明,暴露于 PM2.5 会严重损害卵巢功能。然而,其潜在机制仍是一个谜。本研究证实,通过气管滴注(低剂量为 35 µg/m³,高剂量为 150 µg/m³)接触 PM2.5 5 5 个月后,小鼠卵巢受损,表现为炎性细胞浸润和卵泡闭锁。此外,PM2.5浓度为50、100和150微克/毫升时,暴露24小时会抑制人颗粒细胞(KGN)的细胞活力并诱导细胞凋亡。此外,我们还进行了RNA测序分析,以确定暴露于PM2.5(100 µg/mL)会降低KGN细胞活力的基因和通路。我们发现活化转录因子3(ATF3)明显增加。进一步的机理研究显示,PM2.5 诱导的细胞凋亡、炎症和 ATF3 与其下游的氧化应激信号之间存在密切联系。总之,ATF3 通路在 PM2.5 暴露造成的卵巢损伤中起着至关重要的作用。
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来源期刊
Journal of Ovarian Research
Journal of Ovarian Research REPRODUCTIVE BIOLOGY-
CiteScore
6.20
自引率
2.50%
发文量
125
审稿时长
>12 weeks
期刊介绍: Journal of Ovarian Research is an open access, peer reviewed, online journal that aims to provide a forum for high-quality basic and clinical research on ovarian function, abnormalities, and cancer. The journal focuses on research that provides new insights into ovarian functions as well as prevention and treatment of diseases afflicting the organ. Topical areas include, but are not restricted to: Ovary development, hormone secretion and regulation Follicle growth and ovulation Infertility and Polycystic ovarian syndrome Regulation of pituitary and other biological functions by ovarian hormones Ovarian cancer, its prevention, diagnosis and treatment Drug development and screening Role of stem cells in ovary development and function.
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