Advancing skin model development: A focus on a self-assembled, induced pluripotent stem cell-derived, xeno-free approach.

IF 6.7 1区 工程技术 Q1 CELL & TISSUE ENGINEERING Journal of Tissue Engineering Pub Date : 2024-11-05 eCollection Date: 2024-01-01 DOI:10.1177/20417314241291848
Marla Dubau, Tarada Tripetchr, Lava Mahmoud, Vivian Kral, Burkhard Kleuser
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Abstract

The demand for skin models as alternatives to animal testing has grown due to ethical concerns and the need for accurate substance evaluation. These alternatives, known as New Approach Methodologies (NAMs), are increasingly used for regulatory decisions. Current skin models from primary human cells often rely on bovine collagen, raising ethical issues. This study explores self-assembled skin models (SASM) as a new method, utilizing hair follicle-derived keratinocytes reprogrammed into induced pluripotent stem cells (iPSC) and differentiated into fibroblasts and keratinocytes. The model relies on the ability of fibroblasts to secrete collagen to produce a xeno-free dermal layer and on the differentiation of keratinocytes to create a functional epidermal layer. These layers exhibited confirmed metabolic activity and the capability to withstand test substances. The successful development of SASM underscores the significance of accurate alternatives in dermatological research, providing an ethical and reliable option for substance evaluation and regulatory testing.

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推进皮肤模型开发:聚焦自组装、诱导多能干细胞衍生、无异种方法。
由于道德问题和准确评估物质的需要,对皮肤模型作为动物试验替代品的需求日益增长。这些被称为新方法(NAM)的替代品越来越多地被用于监管决策。目前从原代人类细胞中提取的皮肤模型通常依赖于牛胶原蛋白,从而引发了伦理问题。本研究探索了自组装皮肤模型(SASM)这一新方法,利用毛囊衍生的角质细胞重编程为诱导多能干细胞(iPSC),并分化为成纤维细胞和角质细胞。该模型依靠成纤维细胞分泌胶原蛋白的能力生成无异种真皮层,并依靠角质形成细胞的分化生成功能性表皮层。这些表皮层的新陈代谢活性和承受测试物质的能力都得到了证实。SASM 的成功开发凸显了准确替代品在皮肤病学研究中的重要意义,为物质评估和监管测试提供了一种道德和可靠的选择。
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来源期刊
Journal of Tissue Engineering
Journal of Tissue Engineering Engineering-Biomedical Engineering
CiteScore
11.60
自引率
4.90%
发文量
52
审稿时长
12 weeks
期刊介绍: The Journal of Tissue Engineering (JTE) is a peer-reviewed, open-access journal dedicated to scientific research in the field of tissue engineering and its clinical applications. Our journal encompasses a wide range of interests, from the fundamental aspects of stem cells and progenitor cells, including their expansion to viable numbers, to an in-depth understanding of their differentiation processes. Join us in exploring the latest advancements in tissue engineering and its clinical translation.
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