The first record of Culicoides deltus as a putative vector of Onchocerca flexuosa in Slovak red deer (Cervus elaphus).

IF 1.8 3区 医学 Q2 PARASITOLOGY Parasitology Research Pub Date : 2024-11-05 DOI:10.1007/s00436-024-08386-9
Alica Kočišová, Andrea Schreiberová, Zuzana Kasičová, Nikola Janošková
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Abstract

Within the research conducted in the years 2016-2022 in the area of Volovsky Mountains in Slovakia, 63,950 biting midges were collected during 74 trapping sessions. The aim of the study was to identify species composition of biting midges, their host preference and potential transmission of parasites by these insects under natural conditions. The collected biting midges fell into 29 species and the most common were the Culicoides (C. obsoletus/C. scoticus/C. montanus) that accounted for 47.9% of the collected biting midges. Identification of species was based on the morphology of biting midges and the use of molecular methods. We confirmed positive suckling results on red deer in three samples namely C. montanus, C. scoticus and C. deltus. We examined these samples for the presence of Onchocerca worm DNA. We confirmed the presence of O. flexuosa DNA in one C. deltus sample. The host preference of biting midges was identified by molecular technique that involved sequencing a 350-bp sequence of the mitochondrial cytochrome b gene (cyt b). The presence of Onchocerca flexuosa DNA in C. deltus was confirmed by sequencing of fragments of mitochondrial genes cox1. The sequences matched the previously published sequences for O. flexuosa. Data on high prevalence of infections caused by Onchocerca worms in red deer in Slovakia have already been published and indicated favourable conditions for the vectors and a suitable environment for parasite circulation in Slovakia. According to the authors' knowledge, this was the first ever detection of O. flexuosa in C. deltus in wild nature.

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首次记录到 Culicoides deltus 是斯洛伐克马鹿(Cervus elaphus)中柔性盘尾丝虫病的假定病媒。
2016-2022 年在斯洛伐克沃洛夫斯基山地区开展的研究在 74 次诱捕过程中收集了 63950 只咬蠓。研究的目的是确定咬蠓的物种组成、寄主偏好以及这些昆虫在自然条件下传播寄生虫的可能性。收集到的咬蠓分为 29 个种类,其中最常见的是 Culicoides(C. obsoletus/C. scoticus/C. montanus),占收集到的咬蠓的 47.9%。根据咬蠓的形态和分子方法进行了物种鉴定。我们在三个样本(即 C. montanus、C. scoticus 和 C. deltus)中确认了赤鹿的阳性吸吮结果。我们对这些样本进行了盘尾丝虫 DNA 检测。我们在一个 C. deltus 样本中确认了 O. flexuosa DNA 的存在。咬蠓的寄主偏好是通过分子技术确定的,该技术包括对线粒体细胞色素 b 基因(cyt b)的 350-bp 序列进行测序。通过对线粒体基因 cox1 片段的测序,确认了三角帆栉蠓中存在柔毛蟠尾蠓 DNA。测序结果与之前公布的柔毛盘尾丝虫测序结果一致。斯洛伐克红鹿中盘尾丝虫感染率较高的数据已经公布,这表明斯洛伐克的传播媒介条件有利,寄生虫循环环境适宜。据作者所知,这是首次在野外自然环境中发现C. deltus体内的O. flexuosa。
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来源期刊
Parasitology Research
Parasitology Research 医学-寄生虫学
CiteScore
4.10
自引率
5.00%
发文量
346
审稿时长
6 months
期刊介绍: The journal Parasitology Research covers the latest developments in parasitology across a variety of disciplines, including biology, medicine and veterinary medicine. Among many topics discussed are chemotherapy and control of parasitic disease, and the relationship of host and parasite. Other coverage includes: Protozoology, Helminthology, Entomology; Morphology (incl. Pathomorphology, Ultrastructure); Biochemistry, Physiology including Pathophysiology; Parasite-Host-Relationships including Immunology and Host Specificity; life history, ecology and epidemiology; and Diagnosis, Chemotherapy and Control of Parasitic Diseases.
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