Transcriptomic analysis of ileal adaptations and growth responses in growing hens supplemented with alanyl-glutamine dipeptide.

IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Poultry Science Pub Date : 2024-10-31 DOI:10.1016/j.psj.2024.104479
Usman Nazir, Zhenming Fu, Xucheng Zheng, Muhamamd Hammad Zafar, Zhi Yang, Zhiyue Wang, Haiming Yang
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Abstract

The growing phase of laying hens is crucial for growth and development due to its direct impact on their productivity during laying phase. During initial growth phase, intestinal tract undergoes rapid development which requires plenty of nutrients to help laying hens grow and mature. This study investigated the effect of Alanyl-Glutamine (Aln-Gln) levels on growth performance, ileal morphology and transcriptomic analysis of growing Hy-line brown hens. A total of 480 day old Hy-line brown chicks having similar body weight (BW) were randomly divided to be fed diets having 0%, 0.1%, 0.2% and 0.3% Aln-Gln for 6-wks (8 replicates/group, 15 birds/replicate). One bird from every pen was slaughtered and morphological parameters of ileum were evaluated. Results taken on day 42 revealed an improved average daily gain (ADG), final body weight (FBW) and feed-to-gain ratio (F/G) in the birds that consumed 0.2% and 0.3% Aln-Gln supplemented diet (P < 0.05). Ileal morphological assays showed that villus height, villus width and villus to crypts ratio (V/C) were significantly increased at 42 days of age in birds fed diets with 0.2% Aln-Gln (P<0.05). The RNA sequencing (RNA-Seq) was executed to identify differentially expressed genes (DEGs) among groups that found 2265 DEGs (1256 up-regulated; 1009 down-regulated) in ileum tissue. According to the Kyoto Encyclopedia of Genes (KEGG) and Genomic Pathway Enrichment Analysis, majority of DEGs indicated change in metabolic pathways. Genes related to growth factors, intestinal morphology and protein metabolism were up-regulated in test groups as compared to control group. In conclusion, addition of Aln-Gln to the diet improved growth performance and ileum development in growing hens; transcriptomic analysis revealed up-regulation of genes related to growth and intestinal morphology.

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对补充丙氨酰-谷氨酰胺二肽的生长母鸡回肠适应性和生长反应的转录组分析
蛋鸡的生长阶段对其生长和发育至关重要,因为它直接影响到蛋鸡在产蛋期的生产率。在最初的生长阶段,肠道会迅速发育,需要大量的营养物质来帮助蛋鸡生长和成熟。本研究调查了丙氨酰-谷氨酰胺(Aln-Gln)水平对生长期褐壳蛋鸡的生长性能、回肠形态和转录组分析的影响。将体重(BW)相近的 480 只日龄褐线雏鸡随机分为四组,分别饲喂含 0%、0.1%、0.2% 和 0.3% Aln-Gln 的日粮 6 周(每组 8 个重复,每个重复 15 只鸡)。每栏宰杀一只鸡,评估回肠的形态参数。第 42 天的结果显示,饲喂添加 0.2% 和 0.3% Aln-Gln 的日粮的家禽的平均日增重 (ADG)、最终体重 (FBW) 和饲料与增重比 (F/G) 均有所提高(P < 0.05)。回肠形态测定显示,饲喂添加 0.2% Aln-Gln 的日粮的家禽在 42 日龄时,绒毛高度、绒毛宽度和绒毛与隐窝比率(V/C)均显著增加(P<0.05)。
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来源期刊
Poultry Science
Poultry Science 农林科学-奶制品与动物科学
CiteScore
7.60
自引率
15.90%
发文量
0
审稿时长
94 days
期刊介绍: First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.
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