Latest trends in biosensors powered by nucleic acid isothermal amplification for the diagnosis of joint infections: From sampling to identification towards the point-of-care
Mariarita De Felice , Mariarosaria De Falco , Amina Antonacci , Sara Colella , Vincenzo Vedi , Rachele Isticato , Alfonso Maria Romano , Valeria Nocerino , Bruno Miranda , Luca De Stefano , Viviana Scognamiglio
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引用次数: 0
Abstract
As the number of joint arthroplasties continues to increase, joint infections have emerged as a significant and devastating complication associated with total joint arthroplasty. The formation and persistence of bacterial biofilms create a formidable barrier against both the host immune system and antibiotics, contributing to the pathogenesis of joint infections, antibiotic resistance, and treatment failures. This review examines the latest trends on biosensors powered by isothermal nucleic acid amplification technologies designed to manage joint infections, highlighting their advantages and disadvantages, with a particular attention on their integration into point-of-care testing. In detail, a special focus on such innovative approaches for rapid and specific identification of pathogenic nucleic acids will cover the entire process from sampling to extraction, amplification, and detection methods.
期刊介绍:
TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.