{"title":"A label-free liquid–crystal-droplet based optical sensing platform for the detection of Neisseria gonorrhoeae","authors":"","doi":"10.1016/j.molliq.2024.126410","DOIUrl":null,"url":null,"abstract":"<div><div>An oligonucleotide decorated liquid crystal (LC) droplet platform was studied for the detection of sexually transmitted disease (STD) caused by bacterium <em>Neisseria gonorrhoeae.</em> Homeotropically aligned nematic LC droplets were patterned on the silane modified glass plates and were utilized as sensing platform using an oligonucleotide probe (ssDNA<sub>probe</sub>) sequence specific for <em>N. gonorrhoeae</em>. The developed LC-droplets platform could detect the synthetic complementary DNA (ssDNA<sub>target</sub>) and genomic DNA of <em>N. gonorrhoeae</em> with dark polarizing optical microscopy (POM) images of homeotropic LC alignment up to the lowest detectable limit of 10 pM and 50 pM, respectively. The DNA hybridization event was further monitored by zeta potential (ζ) measurement of LC droplets using dynamic light scattering technique to understand the sensing mechanism of ssDNA<sub>probe</sub> hybridization with ssDNA<sub>target</sub>. The zeta potential response showed a linear relationship to ssDNA<sub>target</sub> over a concentration range of 10 pM to 100 nM and a limit of detection of 10 pM, which is nearly close to limit of detection (LOD) obtained with LC optical response measurement. These results indicated a similar kind of sensing mechanism with LC-droplets in both aqueous medium as well as on a glass substrate. The specificity of the LC droplet platform was investigated with various levels of mismatched synthetic DNA sequences and non-specific genomic DNA isolated from other bacteria. The homeotropic LC optical response with genomic DNA of <em>N. gonorrhoeae</em> in the presence of potential interferents showed that LC-droplet platform is highly selective for <em>N. gonorrhoeae</em>, suggesting a simple, rapid and reliable label-free detection platform.</div></div>","PeriodicalId":371,"journal":{"name":"Journal of Molecular Liquids","volume":null,"pages":null},"PeriodicalIF":5.3000,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Molecular Liquids","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0167732224024693","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0
Abstract
An oligonucleotide decorated liquid crystal (LC) droplet platform was studied for the detection of sexually transmitted disease (STD) caused by bacterium Neisseria gonorrhoeae. Homeotropically aligned nematic LC droplets were patterned on the silane modified glass plates and were utilized as sensing platform using an oligonucleotide probe (ssDNAprobe) sequence specific for N. gonorrhoeae. The developed LC-droplets platform could detect the synthetic complementary DNA (ssDNAtarget) and genomic DNA of N. gonorrhoeae with dark polarizing optical microscopy (POM) images of homeotropic LC alignment up to the lowest detectable limit of 10 pM and 50 pM, respectively. The DNA hybridization event was further monitored by zeta potential (ζ) measurement of LC droplets using dynamic light scattering technique to understand the sensing mechanism of ssDNAprobe hybridization with ssDNAtarget. The zeta potential response showed a linear relationship to ssDNAtarget over a concentration range of 10 pM to 100 nM and a limit of detection of 10 pM, which is nearly close to limit of detection (LOD) obtained with LC optical response measurement. These results indicated a similar kind of sensing mechanism with LC-droplets in both aqueous medium as well as on a glass substrate. The specificity of the LC droplet platform was investigated with various levels of mismatched synthetic DNA sequences and non-specific genomic DNA isolated from other bacteria. The homeotropic LC optical response with genomic DNA of N. gonorrhoeae in the presence of potential interferents showed that LC-droplet platform is highly selective for N. gonorrhoeae, suggesting a simple, rapid and reliable label-free detection platform.
期刊介绍：
The journal includes papers in the following areas:
– Simple organic liquids and mixtures
– Ionic liquids
– Surfactant solutions (including micelles and vesicles) and liquid interfaces
– Colloidal solutions and nanoparticles
– Thermotropic and lyotropic liquid crystals
– Ferrofluids
– Water, aqueous solutions and other hydrogen-bonded liquids
– Lubricants, polymer solutions and melts
– Molten metals and salts
– Phase transitions and critical phenomena in liquids and confined fluids
– Self assembly in complex liquids.– Biomolecules in solution
The emphasis is on the molecular (or microscopic) understanding of particular liquids or liquid systems, especially concerning structure, dynamics and intermolecular forces. The experimental techniques used may include:
– Conventional spectroscopy (mid-IR and far-IR, Raman, NMR, etc.)
– Non-linear optics and time resolved spectroscopy (psec, fsec, asec, ISRS, etc.)
– Light scattering (Rayleigh, Brillouin, PCS, etc.)
– Dielectric relaxation
– X-ray and neutron scattering and diffraction.
Experimental studies, computer simulations (MD or MC) and analytical theory will be considered for publication; papers just reporting experimental results that do not contribute to the understanding of the fundamentals of molecular and ionic liquids will not be accepted. Only papers of a non-routine nature and advancing the field will be considered for publication.
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