Temperature-induced changes in the hematological and biochemical parameters of Nile tilapia anesthetized with Ocimum basilicum

IF 2.2 3区农林科学 Q2 FISHERIES Aquaculture International Pub Date : 2024-11-06 DOI:10.1007/s10499-024-01751-6

Paola Capistrano dos Santos, Emilly Monteiro Lopes, Arlene Sobrinho Ventura, Claudia Andrea Lima Cardoso, Alexandre Vaz da Silva, Domickson Silva Costa, Marília Tedesco, Gabriela Tomas Jerônimo, Maurício Laterça Martins

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Abstract

The study aimed to evaluate the effect of temperatures of 23 and 27 °C on the hematology of Nile tilapia juveniles (Oreochromis niloticus) subjected to anesthesia using the essential oil of basil (Ocimum basilicum) at a concentration of 250 mg L⁻¹. A total of 112 fish were divided into 4 treatments and distributed as follows: fish anesthetized with 250 mg L⁻¹ at a temperature of 23 °C (n = 42); fish anesthetized with 250 mg L⁻¹ at a temperature of 27 °C (n = 42); and for each temperature, a control group fish exposed to diluent solution (95% ethanol) (n = 14, 7 per temperature); and a negative control group fish (water) (n = 14, 7 per temperature). They were exposed to 10 min for both temperatures. Blood collection was performed after the anesthetic induction at each temperature to determine the hematocrit, hemoglobin concentration (Hb), red blood cell count (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH), relative values of leukocytes, and absolute values of leukocytes and thrombocytes, in addition to evaluating plasma chloride and total calcium ions. The analysis of chemical composition by gas chromatography coupled with mass spectrometry (GC–MS) demonstrated methyl-chavicol (70.04%) and linalool (24.59%) as the major components of basil essential oil. Fish anesthetized at a temperature of 23 °C showed lower neutrophil counts (p < 0.05) compared to the other tested groups. For the temperature of 27 °C, values of MCV, MCHC, and RBC decreased (p < 0.05) following exposure to basil essential oil. The calcium ion showed a statistical difference (p < 0.05) between the treatments about the water control group. It is concluded that basil essential oil presented a positive anesthetic effect. The use of O. basilicum essential oil as an anesthetic in Nile tilapia was the best at 23 °C, causing less hematological and biochemical imbalance.

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温度引起的用欧芹麻醉的尼罗罗非鱼血液学和生化指标的变化

该研究旨在评估 23 和 27 °C 的温度对尼罗罗非鱼幼鱼（Oreochromis niloticus）血液学的影响，使用 250 mg L-1 浓度的罗勒（Ocimum basilicum）精油进行麻醉。总共 112 条鱼被分成 4 个处理，分布如下：温度为 23 °C 时使用 250 mg L-1 进行麻醉的鱼（n = 42）；温度为 27 °C 时使用 250 mg L-1 进行麻醉的鱼（n = 42）；每个温度下，对照组鱼暴露于稀释液（95% 乙醇）中（n = 14，每个温度 7 条）；阴性对照组鱼（水）（n = 14，每个温度 7 条）。两种温度下的鱼均暴露 10 分钟。在每个温度下麻醉诱导后采血，测定血细胞比容、血红蛋白浓度（Hb）、红细胞计数（RBC）、平均血球容积（MCV）、平均血球血红蛋白浓度（MCHC）、平均血球血红蛋白（MCH）、白细胞相对值、白细胞和血小板绝对值，以及评估血浆氯离子和总钙离子。气相色谱-质谱（GC-MS）化学成分分析表明，罗勒精油的主要成分是甲基辣椒素（70.04%）和芳樟醇（24.59%）。与其他测试组相比，在 23 °C 温度下麻醉的鱼类中性粒细胞计数较低（p < 0.05）。温度为 27 °C，暴露于罗勒精油后，MCV、MCHC 和 RBC 值下降（p < 0.05）。与水对照组相比，钙离子在不同处理之间存在统计学差异（p < 0.05）。结论是罗勒精油具有积极的麻醉效果。罗勒精油在尼罗罗非鱼中的麻醉效果在 23 °C时最好，引起的血液和生化失衡较少。

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来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.