A visible seedling-stage screening system for the Brassica napus hybrid breeding by a novel hypocotyl length-regulated gene BnHL

IF 10.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Plant Biotechnology Journal Pub Date : 2024-11-06 DOI:10.1111/pbi.14507
Jingyan Fu, Ying Zhang, Meng Yin, Sha Liu, Ziyue Xu, Mingting Wu, Zihan Ni, Peiyao Li, Ruijia Zhu, Guangqin Cai, Maolin Wang, Rui Wang
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Abstract

Rapeseed (Brassica napus) is a globally significant oilseed crop with strong heterosis performance. Recessive genic male sterility (RGMS) is one of the key approaches for utilizing heterosis in B. napus. However, this method faces the inherent challenge of being time-consuming and labour-intensive for removing fertile plants during seed production. Here, we report a hypocotyl length-regulated gene, BnHL, which is closely linked to a known fertility gene, BnMs2, serving as a seedling morphology marker. This marker could be used to identify fertile plants in the breeding of RGMS lines based on hypocotyl traits. By targeting the BnHL gene, both homozygous and heterozygous edited mutants exhibited significantly longer hypocotyls than the wild type (WT). Furthermore, germination experiments revealed that 7 days after seed germination, the difference in hypocotyl length between the mutant and the WT seedlings reached its maximum, effectively distinguishing fertile plants under both white (W) and red/far-red (R/FR) light. Mutations in BnHL did not result in significant changes in main agronomic traits. Thus, this study provides a comprehensive strategy for screening and identifying a new morphological marker gene for early screening in RGMS hybrid breeding with completely non-transgene during the whole production.
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利用新型下胚轴长度调控基因 BnHL 培育甘蓝型油菜杂交种的可视苗期筛选系统
油菜(Brassica napus)是全球重要的油料作物,具有很强的异交性。隐性遗传雄性不育(RGMS)是利用油菜异交的关键方法之一。然而,这种方法面临着固有的挑战,即在种子生产过程中去除可育植株既耗时又耗力。在这里,我们报告了一个下胚轴长度调控基因 BnHL,它与已知的可育基因 BnMs2 紧密相连,可作为幼苗形态标记。该标记可用于在基于下胚轴性状的RGMS品系育种中鉴定可育植株。通过靶向 BnHL 基因,同源和异源编辑突变体的下胚轴都明显长于野生型(WT)。此外,萌发实验显示,种子萌发7天后,突变体与WT幼苗的下胚轴长度差异达到最大值,能有效区分白光(W)和红光/远红光(R/FR)下的可育植株。BnHL 的突变并未导致主要农艺性状的显著变化。因此,本研究为在整个生产过程中完全不转基因的RGMS杂交育种早期筛选和鉴定新的形态标记基因提供了一种综合策略。
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来源期刊
Plant Biotechnology Journal
Plant Biotechnology Journal 生物-生物工程与应用微生物
CiteScore
20.50
自引率
2.90%
发文量
201
审稿时长
1 months
期刊介绍: Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.
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