Bowen Du, Yule Zhang, Fuquan Xie, Zhi Chen, Songrui Wei, Yanqi Ge, Xilin Tian, Qiao Jiang, Qiuliang Wang, Xueji Zhang, Defa Li, Zhongjian Xie, Han Zhang
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引用次数: 0
Abstract
The development of highly precise and rapid biological photonic-integrated chips with single-base resolution for target gene detection holds immense potential to revolutionize genetic diagnostics and nonlinear optical devices. Our recent study presents the design of a single-base resolution photonic-integrated chip (SRPIC) with multichannel biosensing capabilities, enabling discrimination between gene segment sequences differing by a single nucleotide at sample concentrations below 1 fM. Through the utilization of hybrid dielectric–metal nanocavity arrays, the SRPIC established an efficient biosensing platform by motivating light-biological matter coupling behavior. This accomplishment represents an extraordinary 104-fold increase in the limit of detection (LOD) compared with the CRISPR-HOLMES technique, exhibiting nearly impeccable precision during clinical testing. Our research highlights the potential of SRPIC as a powerful tool for the development of high-performance photonic-integrated chips capable of achieving multichannel single-base resolution biosensing, with each channel representing the detection of a single virus.
期刊介绍:
Published as soon as accepted and summarized in monthly issues, ACS Photonics will publish Research Articles, Letters, Perspectives, and Reviews, to encompass the full scope of published research in this field.