Antisecretory Factor 16 (AF16): A Promising Avenue for the Treatment of Traumatic Brain Injury—An In Vitro Model Approach

IF 2.8 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Molecular Neuroscience Pub Date : 2024-11-07 DOI:10.1007/s12031-024-02268-6
Nicola Vahrmeijer, Jurgen Kriel, Bradley M. Harrington, Anton Du Preez van Staden, Adriaan Johannes Vlok, Lize Engelbrecht, Andre Du Toit, Ben Loos
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Abstract

Traumatic brain injury (TBI) is caused by an external mechanical force to the head, resulting in abnormal brain functioning and clinical manifestations. Antisecretory factor (AF16) is a potential therapeutic agent for TBI treatment due to its ability to inhibit fluid secretion and decrease inflammation, intracranial pressure, and interstitial fluid build-up, key hallmarks presented in TBI. Here, we investigated the effect of AF16 in an in vitro model of neuronal injury, as well as its impact on key components of the autophagy pathway and mitochondrial dynamics. N2Awt cells were treated with AF16, injured using a scratch assay, and analysed using confocal microscopy, correlative light and electron microscopy (CLEM), flow cytometry, and western blotting. Our results reveal that AF16 enhances autophagy activity, regulates mitochondrial dynamics, and provides protection as early as 6 h post-injury. Fluorescently labelled AF16 was observed to localise to lysosomes and the autophagy compartment, suggesting a role for autophagy and mitochondrial quality control in conferring AF16-associated neuronal protection. This study concludes that AF16 has potential as a therapeutic agent for TBI treatment through is regulation of autophagy and mitochondrial dynamics.

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抗分泌因子 16 (AF16):治疗创伤性脑损伤的希望之路--一种体外模型方法。
创伤性脑损伤(TBI)是由于头部受到外部机械力的作用,导致大脑功能异常和临床表现异常。抗分泌因子(AF16)能够抑制液体分泌,降低炎症、颅内压和间质积液,是治疗创伤性脑损伤的潜在药物。在这里,我们研究了 AF16 在神经元损伤体外模型中的作用,以及它对自噬途径关键成分和线粒体动力学的影响。用 AF16 处理 N2Awt 细胞,使用划痕试验对其进行损伤,并使用共聚焦显微镜、相关光电子显微镜(CLEM)、流式细胞术和 Western 印迹法对其进行分析。我们的研究结果表明,AF16 能增强自噬活性,调节线粒体动力学,并能在损伤后 6 小时内提供保护。我们观察到荧光标记的 AF16 定位于溶酶体和自噬区,这表明自噬和线粒体质量控制在提供 AF16 相关神经元保护中的作用。本研究的结论是,AF16 通过调节自噬和线粒体动力学,具有治疗创伤性脑损伤的潜力。
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来源期刊
Journal of Molecular Neuroscience
Journal of Molecular Neuroscience 医学-神经科学
CiteScore
6.60
自引率
3.20%
发文量
142
审稿时长
1 months
期刊介绍: The Journal of Molecular Neuroscience is committed to the rapid publication of original findings that increase our understanding of the molecular structure, function, and development of the nervous system. The criteria for acceptance of manuscripts will be scientific excellence, originality, and relevance to the field of molecular neuroscience. Manuscripts with clinical relevance are especially encouraged since the journal seeks to provide a means for accelerating the progression of basic research findings toward clinical utilization. All experiments described in the Journal of Molecular Neuroscience that involve the use of animal or human subjects must have been approved by the appropriate institutional review committee and conform to accepted ethical standards.
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