OsbHLH6, a basic helix-loop-helix transcription factor, confers arsenic tolerance and root-to-shoot translocation in rice.

IF 6.2 1区 生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2024-11-07 DOI:10.1111/tpj.17124
Menghan Huang, Yang Liu, Qianwen Bian, Wenjing Zhao, Juan Zhao, Qingpo Liu
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Abstract

Arsenic (As) is extremely toxic to plants, posing a serious concern for food safety. Identification of genes responsive to As is significative for figuring out this issue. Here, we identified a bHLH transcription factor OsbHLH6 that was involved in mediating the processes of As tolerance, uptake, and root-to-shoot translocation in rice. The expression of OsbHLH6 gene was strongly induced after 3 and 48 h of arsenite [As(III)] treatment. The OsbHLH6-overexpressed transgenic rice (OE-OsbHLH6) was sensitive to, while the knockout mutant of OsbHLH6 gene (Osbhlh6) was tolerant to As(III) stress by affecting the contents of reactive oxygen species (ROS) and non-protein thiols (NPT), etc. Knockout of OsbHLH6 gene increased significantly the As concentration in roots, but decreased extensively As accumulation in shoots, compared to that in OE-OsbHLH6 and WT plants. The transcripts of phytochelatins (PCs) synthetase encoding genes OsPCS1 and OsPCS2, as well as As(III) transporter encoding genes OsLsi1 and OsABCC1 were greatly abundant in Osbhlh6 mutants than in OE-OsbHLH6 and WT plants under As(III) stress. In contrast, the expression of OsLsi2 gene was extensively suppressed by As(III) in Osbhlh6 mutants. OsbHLH6 acted as a transcriptional activator to bind directly to the promoter and regulate the expression of OsPrx2 gene that encodes a peroxidase precursor. Moreover, overexpression of OsbHLH6 gene resulted in significant change of expression of amounts of abiotic stress-related genes, which might partially contribute to the As sensitivity of OE-OsbHLH6 plants. These findings may broaden our understanding of the molecular mechanism of OsbHLH6-mediated As response in rice and provide novel useful genes for rice As stress-resistant breeding.

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OsbHLH6是一种碱性螺旋环螺旋转录因子,能赋予水稻耐砷能力和根到芽的转移。
砷(As)对植物有剧毒,严重威胁食品安全。鉴定对砷有反应的基因对解决这一问题具有重要意义。在这里,我们发现了一个 bHLH 转录因子 OsbHLH6,它参与了水稻对砷的耐受、吸收和根到芽的转位过程。在亚砷酸[As(III)]处理 3 小时和 48 小时后,OsbHLH6 基因的表达被强烈诱导。表达 OsbHLH6 基因的转基因水稻(OE-OsbHLH6)对亚砷酸[As(III)]胁迫敏感,而敲除 OsbHLH6 基因的突变体(Osbhlh6)通过影响活性氧(ROS)和非蛋白质硫醇(NPT)等的含量而对亚砷酸[As(III)]胁迫耐受。与OE-OsbHLH6和WT植株相比,敲除OsbHLH6基因会显著增加根中As的浓度,但会减少芽中As的大量积累。在As(III)胁迫下,Osbhlh6突变体中植物螯合素(PCs)合成酶编码基因OsPCS1和OsPCS2以及As(III)转运体编码基因OsLsi1和OsABCC1的转录量大大高于OE-OsbHLH6和WT植株。相反,OsLsi2基因的表达在Osbhlh6突变体中受到As(III)的广泛抑制。OsbHLH6 作为转录激活因子直接与启动子结合,调控编码过氧化物酶前体的 OsPrx2 基因的表达。此外,OsbHLH6基因的过表达导致非生物胁迫相关基因的表达量发生了显著变化,这可能是OE-OsbHLH6植株对As敏感的部分原因。这些发现可能会拓宽我们对 OsbHLH6 介导的水稻砷反应分子机制的认识,并为水稻抗砷胁迫育种提供新的有用基因。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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