Effects of UV-A/B/C on flavonoids and related synthetic enzymes in Tetrastigma hemsleyanum.

IF 4.1 2区生物学 Q1 PLANT SCIENCES Frontiers in Plant Science Pub Date : 2024-10-23 eCollection Date: 2024-01-01 DOI:10.3389/fpls.2024.1477280

Shan Li, Jingqing Xia, Shouzan Liu, Zhe Li, Qiong Shen, Feng Yang, Xinhong Liu, Yan Bai

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Abstract

Introduction: Tetrastigma hemsleyanum is a folk and rare medicinal plant, and specifically, it is distributed in the south, China. To investigate the cumulative properties of its medicinal components, we examined the effect of UV light on flavonoid content and related enzyme activity changes in T. hemsleyanum.

Methods: The leaves and tubers were treated with UV-A, UV-B and UV-C for 1 h, 1L/23D h, 3 h and 3L/21D h (D represents darkness treatment). High-performance liquid chromatography (HPLC) analysis showed that the content of many flavonoids decreased significantly during UV-A treatment, increased after UV-B and UV-C irradiation and accumulated again after darkness treatment.

Results: In the root tubers of the UV-A group, naringin content in the 3L/21D h group (0.069 μg/g) was 16.30 times higher than that of 3 h group (0.0042 μg/g). The rutin content was elevated after UV irradiation but was not detected in the CK group. The test results of the enzyme-linked kit indicated that the activities of many enzymes were higher in the UV-A and UV-B irradiation groups than those in the CK group, but the results were reversed in the UV-C treatment. After darkness treatment, the activities of most enzymes were higher than those with UV irradiation alone; F3'5'H activity in the 3L/21D h group (97.25 U/L) was 1.24 times higher than that in the 3 h group (78.12 U/L) in the UV-A-treated group.

Discussion: The study results suggest that appropriate UV-B and UV-C irradiation, as well as darkness supplementation, had a promotive effect on flavonoids in the leaves and root tubers of T. hemsleyanum. Additionally, UV irradiation and darkness treatment enhanced the activity of most enzymes.

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紫外线 A/B/C 对 Tetrastigma hemsleyanum 中黄酮类化合物及相关合成酶的影响。

简介Tetrastigma hemsleyanum是一种民间稀有药用植物，主要分布在中国南方。为了研究其药用成分的累积特性，我们研究了紫外线对 Tetrastigma hemsleyanum 中黄酮类化合物含量及相关酶活性变化的影响：方法：用 UV-A、UV-B 和 UV-C 分别处理叶片和块茎 1 h、1L/23D h、3 h 和 3L/21D h（D 代表黑暗处理）。高效液相色谱（HPLC）分析表明，许多黄酮类化合物的含量在紫外线-A处理期间显著下降，在紫外线-B和紫外线-C照射后增加，在黑暗处理后又重新积累：紫外线-A 组块根中，3L/21D h 组的柚皮苷含量（0.069 μg/g）是 3 h 组（0.0042 μg/g）的 16.30 倍。紫外线照射后，芦丁含量升高，但 CK 组未检测到芦丁。酶联试剂盒的检测结果表明，紫外线-A 组和紫外线-B 组中许多酶的活性高于 CK 组，但在紫外线-C 处理中结果相反。黑暗处理后，大多数酶的活性高于单独紫外线照射组；3L/21D h组的F3'5'H活性（97.25 U/L）是紫外线-A处理组3 h组（78.12 U/L）的1.24倍：研究结果表明，适当的紫外线-B和紫外线-C照射以及黑暗条件的补充对T. hemsleyanum叶片和块根中的黄酮类化合物有促进作用。此外，紫外线照射和黑暗处理还能提高大多数酶的活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Plant Science PLANT SCIENCES-

CiteScore

7.30

自引率

14.30%

发文量

4844

审稿时长

14 weeks

期刊介绍： In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches. Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.