Placental extracellular vesicles promoted cervical tumour tissue undergoing death

IF 3 2区 医学 Q2 DEVELOPMENTAL BIOLOGY Placenta Pub Date : 2024-11-02 DOI:10.1016/j.placenta.2024.10.069
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Abstract

Background

Cervical cancer is a leading cause of death in developing countries. Although the placenta is a tumor-like organ, the placental development, including invasive function, is well controlled. One mechanism is that extracellular vesicles (EVs) released from the placenta contribute to this regulation. Placental EVs carry functional proteins and regulatory RNAs. Our previous study reported that placental EVs inhibited ovarian cancer growth in vitro and in vivo.

Methods

Whether the inhibitory effect induced by placental EVs also applies to cervical cancer, a non-endocrine-related cancer, in this study, we first co-cultured the cervical tumour tissues with placental explants.

Results

Co-culturing cervical tumour tissues (n = 7) with placental explants showed necrotic signs and increased levels of senescence-associated proteins and death-associated miRNAs, including miRNA-143-3p, miRNA-519a-5p and miRNA-199a-3p in tumour tissues. Additionally, treatment of HeLa cells with placental EVs reduced the viability of HeLa cells and inhibited the ability of invasion and migration of HeLa cells. Increased levels of senescence-associated proteins and reduced levels of proliferative proteins may contribute to the inhibitory effects in HeLa cells.

Discussion

placental EVs are involved in regulating placental development, and the delivery of cargo significantly impacts the functions of target cells. This study found that factors released from placental explants, likely placental EVs, had anti-tumour effects on the cervical tumour by inhibiting cervical cancer cell viability, invasion, and migration. Cargo in placental EVs, such as cellular death-associated miRNAs, may contribute to the inhibitory effects on cervical tumour.
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胎盘细胞外囊泡促进了宫颈肿瘤组织的死亡。
背景:宫颈癌是发展中国家的主要死因。虽然胎盘是一个肿瘤样器官,但胎盘的发育,包括侵袭功能,都得到了很好的控制。一种机制是胎盘释放的细胞外囊泡 (EVs) 有助于这种调控。胎盘EVs携带功能性蛋白质和调控RNA。我们之前的研究报告称,胎盘 EVs 可抑制卵巢癌在体外和体内的生长:胎盘EVs的抑制作用是否也适用于宫颈癌这种与内分泌无关的癌症,在本研究中,我们首先将宫颈肿瘤组织与胎盘外植体共培养:结果:宫颈肿瘤组织(n = 7)与胎盘外植体共培养后,肿瘤组织出现坏死迹象,衰老相关蛋白和死亡相关 miRNA 水平升高,包括 miRNA-143-3p、miRNA-519a-5p 和 miRNA-199a-3p。此外,用胎盘 EVs 处理 HeLa 细胞可降低 HeLa 细胞的活力,抑制 HeLa 细胞的侵袭和迁移能力。讨论:胎盘 EVs 参与了胎盘发育的调控,货物的运送会对靶细胞的功能产生重大影响。本研究发现,胎盘外植体释放的因子(可能是胎盘EVs)通过抑制宫颈癌细胞的活力、侵袭和迁移,对宫颈肿瘤具有抗肿瘤作用。胎盘EVs中的载体,如细胞死亡相关的miRNAs,可能有助于对宫颈肿瘤的抑制作用。
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来源期刊
Placenta
Placenta 医学-发育生物学
CiteScore
6.30
自引率
10.50%
发文量
391
审稿时长
78 days
期刊介绍: Placenta publishes high-quality original articles and invited topical reviews on all aspects of human and animal placentation, and the interactions between the mother, the placenta and fetal development. Topics covered include evolution, development, genetics and epigenetics, stem cells, metabolism, transport, immunology, pathology, pharmacology, cell and molecular biology, and developmental programming. The Editors welcome studies on implantation and the endometrium, comparative placentation, the uterine and umbilical circulations, the relationship between fetal and placental development, clinical aspects of altered placental development or function, the placental membranes, the influence of paternal factors on placental development or function, and the assessment of biomarkers of placental disorders.
期刊最新文献
Corrigendum to “Unveiling placental development in circadian rhythm-disrupted mice: A photo-acoustic imaging study on unstained tissue” [Placenta 158 (2024) 57-61] Placental extracellular vesicles promoted cervical tumour tissue undergoing death Feto-placental vascular structure and in silico haemodynamics: Of mice, rats, and human Endothelin-1 potentiated constriction in preeclampsia placental veins: Role of ETAR/ETBR/CaV1.2/CALD1 Placenta-associated biomarkers and pregnancy outcome in HPA-1a alloimmunization: A prospective cohort study
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