IF 3 2区医学 Q2 DEVELOPMENTAL BIOLOGY

Placenta

Pub Date : 2025-03-13 DOI: 10.1016/j.placenta.2025.03.009

Bahi Fayek , Yang Doris Liu , Arshdeep Sidhu , Kimia Ziafat , Maya Geerts , Faten F. AbdelHafez , Jefferson Terry , Mohamed A. Bedaiwy

Objectives

This study aimed to examine the influence of chronic intervillositis of unknown etiology (CIUE) on reproductive outcomes in patients with recurrent pregnancy loss (RPL), determine treatment effectiveness in future pregnancies, and estimate the CIUE recurrence rate.

Materials and methods

This retrospective study examined patients with RPL, categorizing them into CIUE and non-CIUE groups. Impact of CIUE on reproductive outcomes was assessed both cross-sectionally and longitudinally. A log-rank survival analysis was conducted to assess the effectiveness of various treatments on achieving ongoing pregnancies or live births following an initial episode of CIUE. Lastly, CIUE recurrence rate was evaluated.

Results

A total of 394 patients were included in the study: 23 in the CIUE group and 371 in the non-CIUE group. No significant differences were observed between the groups regarding baseline characteristics. Adjusted logistic regression showed that patients with CIUE had higher odds of experiencing preterm birth compared to non-CIUE patients (odds ratio [OR] = 3.15; 95 % confidence interval [CI]: 1.07–9.22). With each additional pregnancy, the non-CIUE group had 75 % increased odds of achieving live birth (OR 1.75; 95 % CI: 1.49–2.06), while the CIUE group's increase was not significant (OR 1.32; 95 % CI: 0.86–2.05). Additionally, the odds of stillbirth associated with additional pregnancies increased by 85 % in the CIUE group (OR 1.85; 95 % CI: 1.03–3.36), yet not significant in the non-CIUE group (OR 1.14; 95 % CI: 0.90–1.45). CIUE-treated patients had higher odds of achieving live birth or ongoing pregnancy after 24 months (P = 0.0491). CIUE recurred in 21.7 % of patients.

Conclusion

In the RPL population, CIUE adversely affected the reproductive outcomes. Despite its high recurrence rate, prophylactic treatment showed potential in improving outcomes.

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引用次数: 0

The inhibition of placental mTOR signaling leads to fetal growth restriction with abnormal glucose metabolism in different anatomical regions of placentas 抑制胎盘 mTOR 信号传导会导致胎儿生长受限，并在胎盘的不同解剖区域出现葡萄糖代谢异常

IF 3 2区医学 Q2 DEVELOPMENTAL BIOLOGY

Placenta

Pub Date : 2025-03-12 DOI: 10.1016/j.placenta.2025.03.008

Qian Xu , Jingjing Wang , Yajing Li , Hui Lei , Ni Jin , Jie Lu , Chenxi Qian , Jianhua Zhang , Jie Dong , Xiaohong Wang

Introduction

Fetal growth restriction (FGR) is a common pregnancy complication with significant impact on obstetric and birth outcomes. Increasing evidence shows that the inhibition of placental mechanistic target of rapamycin (mTOR) signaling is closely related to FGR. However, the pathogenesis of FGR is not fully consistent presently, which is subject to the methodological divergence.

Methods

Rapamycin was used to construct the FGR mouse model. Hematoxylin & eosin (HE) and periodic acid-schiff (PAS) staining were used to analyze the morphology of mouse placenta. Western blot was used to analyze the expression levels of glucose transporters and key enzymes associated with glycogen metabolism in human/mouse placental tissues in different anatomic layers. HTR-8 cells were treated with dimethyl sulfoxide (DMSO) or rapamycin (2 mM) for 24 h. Cell viability was detected by CCK8 kit. In addition, glycogen concentration in placental tissue or cell samples was detected by Glycogen Assay Kit.

Results

Firstly, we observed a significant reduction of glucose content in different anatomical regions of human small-for-gestational-age (SGA) placenta, also glucose metabolism was undermined to some extent. Then, we found that FGR placentas showed abnormal morphological changes, the glycogen levels in FGR placentas were significantly reduced by quantitative detection. Meanwhile, the expression levels of glucose transporters, Gys1 and p-Gsk3β in FGR placentas were reduced compared to controls. The HTR-8 cells treated with rapamycin revealed decreasing mTOR activity and glycogen levels. In addition, glucose transporter, GYS1, p-GSK3β expressions were all significantly reduced and t-GSK3β level was significantly elevated.

Discussion

Overall, our data indicate that inhibition of placental mTOR signaling may contribute to the occurrence of FGR by altering glucose metabolism.

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引用次数: 0

Placental weight as a predictor of future health: Insights from a large-scale genome-wide association study 预测未来健康的胎盘重量：大规模全基因组关联研究的启示

IF 3 2区医学 Q2 DEVELOPMENTAL BIOLOGY

Placenta

Pub Date : 2025-03-12 DOI: 10.1016/j.placenta.2025.03.006

Qinyi Zhang , Tianhan Xu , Sihui Yu , Sufang Wu , Ye Yang , Hao Wu , Jiawen Zhang

Introduction

Placental weight has been associated with various adult-onset diseases, but the causal relationships and underlying mechanisms remain unclear.

Methods

This two-sample Mendelian randomization (MR) study utilized genome-wide association study (GWAS) data from multiple independent cohorts, primarily of European ancestry. The analysis included over 1.8 million individuals for type 2 diabetes mellitus (T2DM) outcomes. Data from four independent cohorts were used for validation. The inverse variance-weighted method was used for primary analysis, with weighted median, weighted mode, and MR-Egger regression for sensitivity analyses.

Results

Each standard deviation increase in genetically predicted placental weight was associated with T2DM (β = −0.109, 95 % CI: −0.184 to −0.034), basal cell carcinoma (β = 0.130, 95 % CI: 0.016 to 0.245), acute upper respiratory infections (β = −0.062, 95 % CI: −0.113 to −0.011), neurological diseases (β = −0.009, 95 % CI: −0.014 to −0.003), and endometrial cancer (β = −0.561, 95 % CI: −0.961 to −0.161). Placental weight also showed significant negative associations with blood glucose levels (β = −0.102, 95 % CI: −0.200 to −0.004). Mediation analyses revealed that dried fruit intake mediated 14.68 % of the total effect on T2DM risk, while immune cell phenotype analysis identified HLA DR on CD33dim HLA DR + CD11b + as a potential mediator in the causal pathway.

Conclusion

This study provides genetic evidence for a causal relationship between placental weight and T2DM risk, mediated partly through dietary habits and immune pathways. These findings suggest that early-life placental development may influence long-term metabolic health, highlighting the importance of prenatal care in preventing adult-onset diseases.

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引用次数: 0

Development and validation of a risk prediction model for placental abruption in patients with preeclampsia

IF 3 2区医学 Q2 DEVELOPMENTAL BIOLOGY

Placenta

Pub Date : 2025-03-08 DOI: 10.1016/j.placenta.2025.03.005

Mei Yang, Menghui Wang, Qing Zhu, Nanfang Li

Introduction

To develop a validated risk prediction model for placental abruption in preeclamptic patients with singleton pregnancies firstly.

Methods

Data from 1448 preeclamptic patients with singleton pregnancies who delivered between January 2013 and December 2022 were reviewed. Variables, including demographic characteristics, laboratory test results, comorbidities, and aspirin use were collected and analyzed. The preeclamptic patients were divided into a training set and a validation set according to the time of delivery. Logistic regression with a backward stepwise elimination method was used for variable screening and nomogram construction. The area under the receiver operating characteristic curve and calibration curve were used to evaluate its accuracy. Decision curve analysis and clinical impact curves were conducted to assess predictive performance.

Results

Finally, 1448 preeclamptic patients were included. We collected 50 variables for further analysis. Multivariate logistic regression analysis revealed that severity, subtype, premature rupture of membranes, urinary casts, diastolic blood pressure, aspartate aminotransferase, serum potassium, and fibrin degradation product levels were predictors of placental abruption. These factors were used to construct the nomogram model, which showed good concordance and accuracy. The area under the receiver operating characteristic curve values of the training set and the validation set were 0.767 (95 % CI = 0.728–0.806, P < 0.001) and 0.800 (95 % CI = 0.728–0.872, P < 0.001). Calibration curves revealed significant agreement between the nomogram model and actual observations. Receiver operating characteristic curve analysis and decision curve analysis indicated that the nomogram had good predictive performance.

Discussion

The prediction model can accurately estimate the risk of placental abruption in preeclamptic patients.

方法回顾性分析 2013 年 1 月至 2022 年 12 月间分娩的 1448 例单胎妊娠先兆子痫患者的数据。收集并分析了包括人口统计学特征、实验室检查结果、合并症和阿司匹林使用情况在内的变量。根据分娩时间将先兆子痫患者分为训练集和验证集。在筛选变量和构建提名图时采用了后向逐步排除法的逻辑回归。采用接收者操作特征曲线下面积和校准曲线来评估其准确性。结果最终纳入了 1448 例先兆子痫患者。我们收集了 50 个变量进行进一步分析。多变量逻辑回归分析显示，严重程度、亚型、胎膜早破、尿铸型、舒张压、天冬氨酸氨基转移酶、血清钾和纤维蛋白降解产物水平是胎盘早剥的预测因素。利用这些因素构建的提名图模型显示出良好的一致性和准确性。训练集和验证集的接收者操作特征曲线下面积值分别为0.767（95 % CI = 0.728-0.806, P <0.001）和0.800（95 % CI = 0.728-0.872, P <0.001）。校准曲线显示，提名图模型与实际观察结果之间存在明显的一致性。讨论该预测模型可准确估计子痫前期患者发生胎盘早剥的风险。

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引用次数: 0

METTL3 suppressing SLC31A1 m6A modification regulates trophoblast migration and invasion

IF 3 2区医学 Q2 DEVELOPMENTAL BIOLOGY

Placenta

Pub Date : 2025-03-06 DOI: 10.1016/j.placenta.2025.03.004

Song Wang , Yixiong Lin , Qiong Deng , Xinyang Shen , Qian Chen , Xiaojing Yue , Zhijian Wang

Objective

This article aims to explore the mechanism of METTL3-mediated SLC31A1 N6-methyladenosine (m⁶A) modification affecting trophoblast migration and invasion in preeclampsia (PE).

Methods

The PE model was established using N-nitro-arginine methyl ester induction. Blood pressure was measured on gestation day (GD) 0, 5, 10, 15, and 20, and urine protein concentration on the day before mating and GD 20. HTR-8 SV/neo cells were cultured in vitro and treated with si-METTL3, oe-METTL3, oe-SLC31A1, si-SLC31A1, or RSM3 (METTL3 inhibitor). METTL3 and SLC31A1 were detected by immunohistochemistry and Western blot. After corresponding treatment, HTR-8SV/neo cells were measured for viability, cell damage, proliferation, migration and invasion and apoptotic rate. m⁶A modification level was measured by methylated RNA immunoprecipitation while the interactions between METTL3 and SLC31A1 mRNA, and YTHDF2 and SLC31A1 mRNA was determined by RNA immunoprecipitation.

Results

PE rats showed elevated METTL3 and down-regulated SLC31A1 expression. Treatment with si-METTL3 or oe-SLC31A1 suggested increased cell viability, proliferation, migration and invasion, and reduced cell damage and apoptosis rate, while cells treated with oe-METTL3 or si-SLC31A1 had reversed results. Up-regulating SLC31A1 partially reversed the inhibitory effect of METTL3 on HTR-8SV/neo cell migration and invasion. METTL3 reduced SLC31A1 mRNA stability and inhibited SLC31A1 expression through m⁶A modification in a YTHDF2-dependent manner. Furthermore, the in vivo experiments confirmed that METTL3 promotes PE progression through m⁶A methylation of SLC31A1.

Conclusion

METTL3 reduces SLC31A1 mRNA stability and down-regulates its expression in an m⁶A-YTHDF2-dependent manner, thereby inhibiting trophoblast migration and invasion.

本文旨在探讨METTL3介导的SLC31A1 N6-甲基腺苷（m6A）修饰影响子痫前期（PE）滋养细胞迁移和侵袭的机制。在妊娠第 0、5、10、15 和 20 天测量血压，在交配前一天和妊娠第 20 天测量尿蛋白浓度。体外培养 HTR-8 SV/neo 细胞，并用 si-METTL3、oe-METTL3、oe-SLC31A1、si-SLC31A1 或 RSM3（METTL3 抑制剂）处理。METTL3 和 SLC31A1 通过免疫组化和 Western 印迹进行检测。用甲基化 RNA 免疫沉淀法测定 m6A 修饰水平，用 RNA 免疫沉淀法测定 METTL3 和 SLC31A1 mRNA 之间以及 YTHDF2 和 SLC31A1 mRNA 之间的相互作用。用 si-METTL3 或 oe-SLC31A1 处理大鼠后，细胞活力、增殖、迁移和侵袭增加，细胞损伤和凋亡率降低；而用 oe-METTL3 或 si-SLC31A1 处理大鼠后，结果相反。上调 SLC31A1 部分逆转了 METTL3 对 HTR-8SV/neo 细胞迁移和侵袭的抑制作用。METTL3 降低了 SLC31A1 mRNA 的稳定性，并通过 m6A 修饰以 YTHDF2 依赖性的方式抑制了 SLC31A1 的表达。结论 METTL3 可降低 SLC31A1 mRNA 的稳定性，并以 m6A-YTHDF2 依赖性方式下调其表达，从而抑制滋养细胞的迁移和侵袭。

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引用次数: 0

Expression of co-signaling molecules TIM-3/Galectin-9 at the maternal-fetal interface

IF 3 2区医学 Q2 DEVELOPMENTAL BIOLOGY

Placenta

Pub Date : 2025-03-05 DOI: 10.1016/j.placenta.2025.03.002

Jingliang Xu , Xuqing He , Sujuan Zhang , Li Li , Penghao Li

Introduction

During early pregnancy, fetal placental tissue implants into maternal decidual tissue, forming a unique interface where maternal immune cells do not reject the invading fetal cells. Given the roles of Galectin-9 and Tim-3 in tumor immune regulation, studying their distribution and function at this interface may provide insights into recurrent pregnancy loss.

Methods

This study uses single-cell transcriptomics, spatial transcriptomics, and multiplex immunohistochemistry to examine the expression and localization of Galectin-9 and TIM-3. Hormone-induced decidualization of immortalized human endometrial stromal cells was conducted to investigate Galectin-9 expression.

Results

The major immune cells in the maternal decidua, such as T cells, NK cells, and macrophages, co-express Galectin-9 and TIM-3. Unlike TIM-3, Galectin-9 is also highly expressed in endothelial cells and decidualized stromal cells. Among placenta-derived cells, Hofbauer cells (HBs) and Placenta-associated maternal monocytes/macrophages (PAMMs) exhibit high expression of both Galectin-9 and TIM-3, while trophoblast cells show relatively low levels of expression. Additionally, hormone-induced decidualization significantly upregulates Galectin-9 expression in endometrial stromal cells.

Discussion

The research results suggest that Galectin-9 and TIM-3, as important immune co-signaling molecules, may play a crucial role in maintaining the immune-tolerant microenvironment at the maternal-fetal interface. Additionally, the association between decidualization and Galectin-9 expression reveals its potential role in pregnancy maintenance, providing new insights for the study of adverse pregnancy outcomes.

{"title":"Expression of co-signaling molecules TIM-3/Galectin-9 at the maternal-fetal interface","authors":"Jingliang Xu , Xuqing He , Sujuan Zhang , Li Li , Penghao Li","doi":"10.1016/j.placenta.2025.03.002","DOIUrl":"10.1016/j.placenta.2025.03.002","url":null,"abstract":"<div><h3>Introduction</h3><div>During early pregnancy, fetal placental tissue implants into maternal decidual tissue, forming a unique interface where maternal immune cells do not reject the invading fetal cells. Given the roles of Galectin-9 and Tim-3 in tumor immune regulation, studying their distribution and function at this interface may provide insights into recurrent pregnancy loss.</div></div><div><h3>Methods</h3><div>This study uses single-cell transcriptomics, spatial transcriptomics, and multiplex immunohistochemistry to examine the expression and localization of Galectin-9 and TIM-3. Hormone-induced decidualization of immortalized human endometrial stromal cells was conducted to investigate Galectin-9 expression.</div></div><div><h3>Results</h3><div>The major immune cells in the maternal decidua, such as T cells, NK cells, and macrophages, co-express Galectin-9 and TIM-3. Unlike TIM-3, Galectin-9 is also highly expressed in endothelial cells and decidualized stromal cells. Among placenta-derived cells, Hofbauer cells (HBs) and Placenta-associated maternal monocytes/macrophages (PAMMs) exhibit high expression of both Galectin-9 and TIM-3, while trophoblast cells show relatively low levels of expression. Additionally, hormone-induced decidualization significantly upregulates Galectin-9 expression in endometrial stromal cells.</div></div><div><h3>Discussion</h3><div>The research results suggest that Galectin-9 and TIM-3, as important immune co-signaling molecules, may play a crucial role in maintaining the immune-tolerant microenvironment at the maternal-fetal interface. Additionally, the association between decidualization and Galectin-9 expression reveals its potential role in pregnancy maintenance, providing new insights for the study of adverse pregnancy outcomes.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"163 ","pages":"Pages 43-50"},"PeriodicalIF":3.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal immune activation elicits rapid and sex-dependent changes in gene expression and vascular dysfunction in the rat placenta

IF 3 2区医学 Q2 DEVELOPMENTAL BIOLOGY

Placenta

Pub Date : 2025-03-04 DOI: 10.1016/j.placenta.2025.03.001

Erin Biggar , Ruth Thomas , Megan L. Lave , Gargi Jaju Bhattad , Nagalingam Rajakumar , Stephen J. Renaud

Introduction

Maternal immune activation (MIA), characterized by increased circulating inflammatory mediators during pregnancy, is associated with adverse pregnancy outcomes and neurodevelopmental deficits in offspring. These health outcomes often manifest differently depending on fetal-placental sex. A well-established model of MIA involves administration of a viral mimetic, polyinosinic:polycytidilic acid (PolyI:C), to pregnant rodents. Placental responses to PolyI:C contribute to the detrimental effects of MIA on offspring, but these responses have not yet been well characterized. In the present study, we profiled acute gene expression changes in male and female placentas following PolyI:C administration to pregnant rats during late gestation.

Methods

Pregnant rats received 4 mg/kg PolyI:C or saline intravenously on gestational day 18.5, and tissues were harvested 4–5 h later. Gene expression profiling on placental tissue was performed. Enzyme immunoassays and immunohistochemistry were conducted to determine levels of select proteins in maternal blood and placental tissue, respectively.

Results

Maternal PolyI:C exposure caused a robust increase in levels of inflammatory mediators in maternal blood and placental tissue. There were more genes differentially expressed in female placentas after PolyI:C exposure (765) than male placentas (221), including reduced expression of genes associated with maternal-fetal communication. Placentas also had increased expression of genes linked with vascular dysfunction after PolyI:C-induced MIA.

Discussion

PolyI:C elicited a powerful inflammatory response in the placenta along with vascular dysfunction, likely contributing to the adverse pregnancy outcomes triggered by MIA. Female placentas responded to PolyI:C more vigorously than male placentas, which could underlie the differential outcomes of MIA depending on sex.

{"title":"Maternal immune activation elicits rapid and sex-dependent changes in gene expression and vascular dysfunction in the rat placenta","authors":"Erin Biggar , Ruth Thomas , Megan L. Lave , Gargi Jaju Bhattad , Nagalingam Rajakumar , Stephen J. Renaud","doi":"10.1016/j.placenta.2025.03.001","DOIUrl":"10.1016/j.placenta.2025.03.001","url":null,"abstract":"<div><h3>Introduction</h3><div>Maternal immune activation (MIA), characterized by increased circulating inflammatory mediators during pregnancy, is associated with adverse pregnancy outcomes and neurodevelopmental deficits in offspring. These health outcomes often manifest differently depending on fetal-placental sex. A well-established model of MIA involves administration of a viral mimetic, polyinosinic:polycytidilic acid (PolyI:C), to pregnant rodents. Placental responses to PolyI:C contribute to the detrimental effects of MIA on offspring, but these responses have not yet been well characterized. In the present study, we profiled acute gene expression changes in male and female placentas following PolyI:C administration to pregnant rats during late gestation.</div></div><div><h3>Methods</h3><div>Pregnant rats received 4 mg/kg PolyI:C or saline intravenously on gestational day 18.5, and tissues were harvested 4–5 h later. Gene expression profiling on placental tissue was performed. Enzyme immunoassays and immunohistochemistry were conducted to determine levels of select proteins in maternal blood and placental tissue, respectively.</div></div><div><h3>Results</h3><div>Maternal PolyI:C exposure caused a robust increase in levels of inflammatory mediators in maternal blood and placental tissue. There were more genes differentially expressed in female placentas after PolyI:C exposure (765) than male placentas (221), including reduced expression of genes associated with maternal-fetal communication. Placentas also had increased expression of genes linked with vascular dysfunction after PolyI:C-induced MIA.</div></div><div><h3>Discussion</h3><div>PolyI:C elicited a powerful inflammatory response in the placenta along with vascular dysfunction, likely contributing to the adverse pregnancy outcomes triggered by MIA. Female placentas responded to PolyI:C more vigorously than male placentas, which could underlie the differential outcomes of MIA depending on sex.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"163 ","pages":"Pages 51-60"},"PeriodicalIF":3.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction of clinical risk factors in pregnancy using optimized neural network scheme

IF 3 2区医学 Q2 DEVELOPMENTAL BIOLOGY

Placenta

Pub Date : 2025-03-04 DOI: 10.1016/j.placenta.2025.03.003

C. Jeyalakshmi , G. Bhavani

Women should be aware of prenancy related health issues. A user-friendly model is developed in which the patients can use as well as clinicians to determine the risks associated with foetal development inside the womb, birth weight, whose effects are typically linked to the mother through biological relationships. Recent advances in computer vision and artificial intelligence offer new techniques for automated evaluation of medical images across a variety of fields, including ultrasound (US) images. Enhancing the detection of the estimated foetal weight (EFW) and mother-foetal disease computations can aid obstetricians in making decisions and reduce perinatal issues. This study aims to build a birth weight classification and prediction of relevant parameters during delivery. In this data analysis suite, exploratory data analysis is performed as part of the data pre-processing to investigate the fundamental information and transformational properties. For feature extracting model, the Advanced Dynamic based Feature Selection (ADFS) algorithm has been used which is optimized using the enriched elephant herding optimization algorithm (EEHOA). The multiple feature estimation is classified using augmented recurrent neural network classifier (AURNN). The findings of analyses with graphical representations have been interpreted through the application of visual analytical techniques.

{"title":"Prediction of clinical risk factors in pregnancy using optimized neural network scheme","authors":"C. Jeyalakshmi , G. Bhavani","doi":"10.1016/j.placenta.2025.03.003","DOIUrl":"10.1016/j.placenta.2025.03.003","url":null,"abstract":"<div><div>Women should be aware of prenancy related health issues. A user-friendly model is developed in which the patients can use as well as clinicians to determine the risks associated with foetal development inside the womb, birth weight, whose effects are typically linked to the mother through biological relationships. Recent advances in computer vision and artificial intelligence offer new techniques for automated evaluation of medical images across a variety of fields, including ultrasound (US) images. Enhancing the detection of the estimated foetal weight (EFW) and mother-foetal disease computations can aid obstetricians in making decisions and reduce perinatal issues. This study aims to build a birth weight classification and prediction of relevant parameters during delivery. In this data analysis suite, exploratory data analysis is performed as part of the data pre-processing to investigate the fundamental information and transformational properties. For feature extracting model, the Advanced Dynamic based Feature Selection (ADFS) algorithm has been used which is optimized using the enriched elephant herding optimization algorithm (EEHOA). The multiple feature estimation is classified using augmented recurrent neural network classifier (AURNN). The findings of analyses with graphical representations have been interpreted through the application of visual analytical techniques.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"163 ","pages":"Pages 33-42"},"PeriodicalIF":3.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal background and perinatal complications in MCI: A retrospective cohort study

IF 3 2区医学 Q2 DEVELOPMENTAL BIOLOGY

Placenta

Pub Date : 2025-02-27 DOI: 10.1016/j.placenta.2025.02.017

Tatsuya Yoshihara, Yasuhiko Okuda, So Owada, Yosuke Ono, Satoko Sasatsu, Maki Ogi, Eriko Ogasahara, Osamu Yoshino

Objective

Marginal cord insertion (MCI) is often defined as an abnormal placental cord insertion (PCI), yet there is limited discussion on the maternal backgrounds and perinatal complications associated with its occurrence. This retrospective cohort study aimed to investigate maternal backgrounds associated with MCI and to compare perinatal outcomes between MCI and normal PCI.

Materials and methods

The study included 1038 deliveries from 2021 to 2023 in our institution, examining maternal backgrounds and perinatal outcomes. Multivariable logistic regression analysis was conducted for variables that showed significance in univariate analysis of maternal backgrounds. For perinatal outcomes, variables that exhibited significance were further analyzed using multivariable logistic regression, considering factors previously reported to be associated with those events.

Results

9.5 % exhibited MCI. Assisted reproductive technology, nulliparous, and congenital uterine anomalies were identified as independent risk factors for MCI. In perinatal outcomes, fetal growth restriction (FGR) and emergency cesarean section were significantly more prevalent in cases with MCI. Even when compared to factors previously reported to be associated with FGR and emergency cesarean section, MCI remained an independent risk factor.

Conclusion

In addition to previously reported factors such as ART and primiparity, uterine anomalies were also identified as risk factors for MCI. It is important to manage MCI with the awareness that it increases the incidence of perinatal complications.

{"title":"Maternal background and perinatal complications in MCI: A retrospective cohort study","authors":"Tatsuya Yoshihara, Yasuhiko Okuda, So Owada, Yosuke Ono, Satoko Sasatsu, Maki Ogi, Eriko Ogasahara, Osamu Yoshino","doi":"10.1016/j.placenta.2025.02.017","DOIUrl":"10.1016/j.placenta.2025.02.017","url":null,"abstract":"<div><h3>Objective</h3><div>Marginal cord insertion (MCI) is often defined as an abnormal placental cord insertion (PCI), yet there is limited discussion on the maternal backgrounds and perinatal complications associated with its occurrence. This retrospective cohort study aimed to investigate maternal backgrounds associated with MCI and to compare perinatal outcomes between MCI and normal PCI.</div></div><div><h3>Materials and methods</h3><div>The study included 1038 deliveries from 2021 to 2023 in our institution, examining maternal backgrounds and perinatal outcomes. Multivariable logistic regression analysis was conducted for variables that showed significance in univariate analysis of maternal backgrounds. For perinatal outcomes, variables that exhibited significance were further analyzed using multivariable logistic regression, considering factors previously reported to be associated with those events.</div></div><div><h3>Results</h3><div>9.5 % exhibited MCI. Assisted reproductive technology, nulliparous, and congenital uterine anomalies were identified as independent risk factors for MCI. In perinatal outcomes, fetal growth restriction (FGR) and emergency cesarean section were significantly more prevalent in cases with MCI. Even when compared to factors previously reported to be associated with FGR and emergency cesarean section, MCI remained an independent risk factor.</div></div><div><h3>Conclusion</h3><div>In addition to previously reported factors such as ART and primiparity, uterine anomalies were also identified as risk factors for MCI. It is important to manage MCI with the awareness that it increases the incidence of perinatal complications.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"163 ","pages":"Pages 29-32"},"PeriodicalIF":3.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaginal miR-210-3p as a potential biomarker for pregnancies complicated by early fetal growth restriction: A proof-of-concept case-control study

IF 3 2区医学 Q2 DEVELOPMENTAL BIOLOGY

Placenta

Pub Date : 2025-02-25 DOI: 10.1016/j.placenta.2025.02.015

Elisabetta Bigagli , Elisa Spataro , Lucia Pasquini , Lorenzo Cinci , Mario D'Ambrosio , Chiara De Blasi , Chiara Bartolini , Felice Petraglia , Cristina Luceri

Introduction

Fetal growth restriction (FGR) is associated with increased risk of neonatal morbidity and mortality or long-term adverse outcomes. We investigated the ability of hypoxia and angiogenesis-related miR-210-3p and miR-126-5p to identify early FGR cases and their correlations with neonatal outcomes.

Methods

Twenty-nine women with pregnancies complicated by early FGR diagnosis and 25 controls matched for gestational age (GA) were enrolled and their vaginal fluid (VF) and plasma were collected. MiR-210-3p and miR-126-5p were measured by RT-qPCR and their targets were identified by in-silico analysis limited only to those already experimentally validated in other contexts.

Results

Overall, VF levels of miR-210-3p were lower in early FGR cases compared to controls (p < 0.05). miR-210-3p was lower in severe cases and in women who later developed preeclampsia (p < 0.05). VF miR-210-3p levels correlated with lower birth weight, premature birth and severe complications at birth (p < 0.05). miR-210-3p was not detected in plasma and no correlations were observed between miR-126-5p and FGR or neonatal outcomes. In silico analyses identified HIF-1α, HIF-3α, BDNF, IGFBP3, RAD52 and TWIST-1 as experimentally validated targets of miR-210-3p. Among the predicted biological pathways controlled by miR-210-3p, we found hypoxia-responsive signaling such as autophagy, oxidative stress and metabolic pathways.

Discussion

Although validation is needed, these findings suggest that VF levels of miR-210-3p may potentially serve as biomarker for the diagnosis of early FGR; future mechanistic studies are also advisable to investigate whether pharmacological strategies based on miR-210-3p, or its downstream targets may be useful for FGR.

{"title":"Vaginal miR-210-3p as a potential biomarker for pregnancies complicated by early fetal growth restriction: A proof-of-concept case-control study","authors":"Elisabetta Bigagli , Elisa Spataro , Lucia Pasquini , Lorenzo Cinci , Mario D'Ambrosio , Chiara De Blasi , Chiara Bartolini , Felice Petraglia , Cristina Luceri","doi":"10.1016/j.placenta.2025.02.015","DOIUrl":"10.1016/j.placenta.2025.02.015","url":null,"abstract":"<div><h3>Introduction</h3><div>Fetal growth restriction (FGR) is associated with increased risk of neonatal morbidity and mortality or long-term adverse outcomes. We investigated the ability of hypoxia and angiogenesis-related miR-210-3p and miR-126-5p to identify early FGR cases and their correlations with neonatal outcomes.</div></div><div><h3>Methods</h3><div>Twenty-nine women with pregnancies complicated by early FGR diagnosis and 25 controls matched for gestational age (GA) were enrolled and their vaginal fluid (VF) and plasma were collected. MiR-210-3p and miR-126-5p were measured by RT-qPCR and their targets were identified by in-silico analysis limited only to those already experimentally validated in other contexts.</div></div><div><h3>Results</h3><div>Overall, VF levels of miR-210-3p were lower in early FGR cases compared to controls (p < 0.05). miR-210-3p was lower in severe cases and in women who later developed preeclampsia (p < 0.05). VF miR-210-3p levels correlated with lower birth weight, premature birth and severe complications at birth (p < 0.05). miR-210-3p was not detected in plasma and no correlations were observed between miR-126-5p and FGR or neonatal outcomes. In silico analyses identified HIF-1α, HIF-3α, BDNF, IGFBP3, RAD52 and TWIST-1 as experimentally validated targets of miR-210-3p. Among the predicted biological pathways controlled by miR-210-3p, we found hypoxia-responsive signaling such as autophagy, oxidative stress and metabolic pathways.</div></div><div><h3>Discussion</h3><div>Although validation is needed, these findings suggest that VF levels of miR-210-3p may potentially serve as biomarker for the diagnosis of early FGR; future mechanistic studies are also advisable to investigate whether pharmacological strategies based on miR-210-3p, or its downstream targets may be useful for FGR.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"163 ","pages":"Pages 8-15"},"PeriodicalIF":3.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143512092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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