Quorum sensing: the “switch” in the competitive relationship between Gram-positive bacteria based on transcriptomic analysis

IF 6.1 1区 生物学 Q1 MICROBIOLOGY Microbiological research Pub Date : 2024-10-31 DOI:10.1016/j.micres.2024.127961
Guoxu Ao , Changli Wang , Liguo Yang , Yue Ma , Zhaoxuan Wang , Yueqi Shi , Shanshan Sun , Wenxiang Ping
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Abstract

Competition phenomenon is widely presented in nature, however, few reports on the competition phenomenon between bacteria based on the perspective of quorum sensing (QS), especially between Gram-positive bacteria. Here, the Gram-positive bacteria Rhodococcus sp. HD1 and Microbacterium sp. HM-2 were co-cultured, and the epiphysiological indicators, transcriptomics combined with gene engineering technique were applied to clarify the role of QS in the competition between Gram-positive bacteria. The results showed that the morphology of strain HD1 was changed into ellipsoids from long rods, the surface-to-volume ratio increased, and the competition index increased within strains HM-2 and HD1. The biomass of strain HD1(8.06×107 CFU/mL) was decreased significantly (p<0.05) under co-culture system, compared with mono-culture (5.75×108 CFU/mL), indicating that strain HM-2 had an inhibitory effect on HD1 at 12 h. Transcriptomic analysis revealed that QS-related genes were highly expressed in strain HM-2, and the expression level of the virulence gene TM_0352 was the highest (FPKM: 1774.19). Meanwhile, the ABC transporters-related genes in strain HD1 were significantly increased. Furthermore, QS pathway-related genes in strain HM-2 and ABC transporters-related genes in strain HD1 showed a significant correlation with the gene TM_0352 expression by the Mantel test analysis (p<0.05), surmising that the TM_0352 gene played a dominant role in the co-culture system. Knockout and complementation experiments confirmed that the function of gene TM_0352. The structural equation model showed that the QS up-regulation of strain HM-2 significantly promoted the expression of virulence genes, while strain HD1 promoted ABC transporters to cope with the up-regulation of TM_0352. The up-regulation of TM_0352 promoted the biomass of strain HM-2 and inhibited the biomass of HD1.The above results displayed that the competition phenomenon appeared by QS driving the up-regulation of TM_0352 gene in strain HM-2, which led to the up-regulation of ABC transporters in strain HD1. And these findings provided new insights into the perspective of factors related to competition inhibition between bacteria.
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法定人数感应:基于转录组分析的革兰氏阳性细菌竞争关系中的 "开关"。
竞争现象在自然界中广泛存在,但基于法定量感应(QS)视角的细菌间竞争现象,尤其是革兰氏阳性菌间竞争现象的报道却很少。本文以革兰氏阳性菌 Rhodococcus sp. HD1 和 Microbacterium sp. HM-2 为研究对象,应用表观生理指标、转录组学和基因工程技术,阐明了 QS 在革兰氏阳性菌竞争中的作用。结果表明,菌株HD1的形态由长杆变为椭圆形,表面体积比增加,菌株HM-2和HD1内部的竞争指数增加。与单株培养(5.75×108 CFU/mL)相比,共培养系统下菌株 HD1 的生物量(8.06×107 CFU/mL)显著下降(p<0.05),表明菌株 HM-2 在 12 h 内对 HD1 有抑制作用。转录组分析表明,QS相关基因在菌株HM-2中高表达,其中毒力基因TM_0352的表达量最高(FPKM:1774.19)。同时,菌株 HD1 中 ABC 转运体相关基因的表达量也明显增加。此外,经 Mantel 检验分析,菌株 HM-2 中的 QS 通路相关基因和菌株 HD1 中的 ABC 转运体相关基因与基因 TM_0352 的表达有明显的相关性(p
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来源期刊
Microbiological research
Microbiological research 生物-微生物学
CiteScore
10.90
自引率
6.00%
发文量
249
审稿时长
29 days
期刊介绍: Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.
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