Comparative transcriptomic analysis revealed potential mechanisms regulating the hypertrophy of goose pectoral muscles.

IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Poultry Science Pub Date : 2024-11-02 DOI:10.1016/j.psj.2024.104498
Xinyue Hu, Yali Liu, Bincheng Tang, Jiwei Hu, Hua He, Hehe Liu, Liang Li, Shenqiang Hu, Jiwen Wang
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Abstract

Pectoral muscle development is an important economic trait. According to the different essence, muscle development can be divided into 2 processes: embryonic muscle fiber generation and postnatal muscle fiber hypertrophy, and postnatal muscle fiber hypertrophy has a greater impact on muscle development than the number of muscle fibers formed during the embryonic phase in poultry. However, the underlying mechanisms regulating the hypertrophy of goose pectoral muscles have not been elucidated. Therefore, the purpose of the present study was to conduct transcriptome sequencing in pectoral muscles of both Landes (LD) and Sichuan White (SW) geese at 6, 10, and 30 weeks of age to reveal the molecular mechanisms regulating pectoral muscle hypertrophy through intra-breed and inter-breed bioinformatics analyses. Phenotypically, the pectoral muscle weight/index of LD and SW geese increased from 6 to 30 weeks of age, and except for the pectoral muscle index at 10 weeks of age (P = 0.962), at the same age, the pectoral muscle weight/index of LD geese were significantly higher than that of SW geese (P < 0.05). In transcriptional regulation, intra-breed bioinformatics analysis identified 3331 genes whose expression levels were opposite to the trend of pectoral muscle hypertrophy both in LD and SW geese, and the 3331 genes were mainly enriched into abundant KEGG pathways related to lipid metabolism, proliferation/apoptosis, and immune response. Moreover, 23 genes (including SLC2A10, TNFRSF1A, PRKAA1, SLC27A4, ITGB2, THY1, RHOA, MYL10, ACTB, PRKCB, PIK3R2, RAC2, DMD, LATS2, YAP1, WWTR1, SMAD7, CTGF, FGF1, AXIN2, GLI2, ID2, and CCND2) who were enriched in 6 crosstalk pathways named viral myocarditis, insulin resistance, sphingolipid signaling pathway, hippo signaling pathway, chemokine signaling pathway, and leukocyte transendothelial migration were identified as the key candidate genes regulating the hypertrophy of goose pectoral muscles. In inter-breed bioinformatics analysis, abundant different expression genes (DEGs) related to lipid metabolism, immune response, and proliferation/apoptosis were identified between LD and SW geese too, and compared with SW geese, the expression level of MYL10 in LD geese was lower, while the expression levels of GLI2/CTGF/SMAD7 in LD geese were higher. These results suggested that the hypertrophy of goose pectoral muscles might be achieved through more lipid deposition and less leukocyte infiltration to promote the proliferation of cells within the muscles, and the low expression of MYL10 and high expressions of GLI2/CTGF/SMAD7 might the keys to induce the pectoral muscle hypertrophy of LD geese from 6 to 30 weeks of age over that of SW geese. All data the present study obtained will provide new insights into the molecular mechanisms regulating the hypertrophy of goose pectoral muscles.

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比较转录组分析揭示了调节鹅胸肌肥大的潜在机制。
胸肌发育是一项重要的经济性状。根据不同的本质,肌肉发育可分为胚胎期肌纤维生成和产后肌纤维肥大两个过程,而产后肌纤维肥大对家禽肌肉发育的影响要大于胚胎期形成的肌纤维数量。然而,调节鹅胸肌肥大的内在机制尚未阐明。因此,本研究的目的是对6周龄、10周龄和30周龄的兰德斯鹅(LD)和四川白鹅(SW)的胸肌进行转录组测序,通过种内和种间生物信息学分析揭示调控胸肌肥大的分子机制。从表型上看,6-30周龄LD鹅和SW鹅的胸肌重量/指数均有所增加,除10周龄的胸肌指数(P = 0.962)外,同龄LD鹅的胸肌重量/指数均显著高于SW鹅(P < 0.05)。在转录调控方面,种内生物信息学分析发现3331个基因的表达水平与LD鹅和SW鹅胸肌肥大的趋势相反,这3331个基因主要富集在与脂质代谢、增殖/凋亡和免疫应答相关的丰富的KEGG通路中。此外,23个基因(包括SLC2A10、TNFRSF1A、PRKAA1、SLC27A4、ITGB2、THY1、RHOA、MYL10、ACTB、PRKCB、PIK3R2、RAC2、DMD、LATS2、YAP1、WWTR1、SMAD7、CTGF、FGF1、AXIN2、GLI2、ID2 和 CCND2),它们在 6 个名为病毒性心肌炎的串联通路中富集、胰岛素抵抗、鞘脂信号通路、河马信号通路、趋化因子信号通路和白细胞跨内皮迁移这 6 个交叉通路中富集的基因被确定为调控鹅胸肌肥大的关键候选基因。在种间生物信息学分析中,也发现了大量与脂质代谢、免疫反应和增殖/凋亡相关的不同表达基因(DEGs),与西南鹅相比,MYL10在西南鹅中的表达水平较低,而GLI2/CTGF/SMAD7在西南鹅中的表达水平较高。这些结果表明,鹅胸肌的肥大可能是通过更多的脂质沉积和更少的白细胞浸润来促进肌肉内细胞的增殖,而MYL10的低表达和GLI2/CTGF/SMAD7的高表达可能是诱导6-30周龄LD鹅胸肌肥大超过SW鹅的关键。本研究获得的所有数据将为调控鹅胸肌肥大的分子机制提供新的见解。
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来源期刊
Poultry Science
Poultry Science 农林科学-奶制品与动物科学
CiteScore
7.60
自引率
15.90%
发文量
0
审稿时长
94 days
期刊介绍: First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.
期刊最新文献
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