Arabidopsis GDH1 and GDH2 genes double knock-out results in a stay-green phenotype during dark-induced senescence.

IF 3.4 3区 生物学 Q1 PLANT SCIENCES Physiology and Molecular Biology of Plants Pub Date : 2024-10-01 Epub Date: 2024-10-17 DOI:10.1007/s12298-024-01517-7
Elena Yu Garnik, Daria V Vilyanen, Anfisa A Vlasova, Vladislav I Tarasenko, Yuri M Konstantinov
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Abstract

Yellowing is the first visually observable sign of plant leaf senescence. We found that Arabidopsis double knockout mutant gdh1gdh2 for genes of NAD(H)-dependent glutamate dehydrogenase retains green color of the leaves (stay-green phenotype) during a dark-induced senescence, in contrast to wild-type plants, whose leaves turn yellow. When the gdh1gdh2 plants are exposed to the dark more than four days, they demonstrate slower chlorophyll degradation than in the wild-type plants under the same conditions, as well as dysregulation of chlorophyll breakdown genes encoding chlorophyll b reductase, Mg-dechelatase, pheophytinase and pheophorbide a oxygenase. The slowed degradation of chlorophyll b in gdh1gdh2 plants significantly alters the chlorophyll a/b ratio. Ion leakage in the mutant plants increases significantly from four to eight days in the darkness, correlating with their premature death during this period. The discovered facts suggest a functional connection between activity of NAD(H)-dependent glutamate dehydrogenase and dark-induced senescence progress in Arabidopsis.

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拟南芥 GDH1 和 GDH2 基因双基因敲除会导致在黑暗诱导的衰老过程中出现留绿表型。
黄化是植物叶片衰老的第一个直观迹象。我们发现,拟南芥 NAD(H)依赖型谷氨酸脱氢酶基因的双基因敲除突变体 gdh1gdh2 在黑暗诱导的衰老过程中叶片仍保持绿色(留绿表型),而野生型植株的叶片则会变黄。当 gdh1gdh2 植物暴露在黑暗中超过四天时,它们的叶绿素降解速度比相同条件下的野生型植物慢,叶绿素 b 还原酶、镁脱螯酶、叶绿素酶和叶绿素 a 加氧酶的叶绿素分解基因也出现失调。gdh1gdh2 植物叶绿素 b 的降解速度减慢,显著改变了叶绿素 a/b 的比例。突变体植物的离子泄漏在黑暗中4到8天明显增加,这与它们在此期间过早死亡有关。这些发现表明,拟南芥中依赖 NAD(H)的谷氨酸脱氢酶的活性与黑暗诱导的衰老进程之间存在功能性联系。
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来源期刊
CiteScore
7.10
自引率
0.00%
发文量
126
期刊介绍: Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.
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