Effect of high mobility group box 1 pathway inhibition on gene expression in the prefrontal cortex of mice exposed to alcohol.

Shuxun Qiu, ShuXin Dong, Jingxin Fan, Wu Chengji, Xunzhong Qi
{"title":"Effect of high mobility group box 1 pathway inhibition on gene expression in the prefrontal cortex of mice exposed to alcohol.","authors":"Shuxun Qiu, ShuXin Dong, Jingxin Fan, Wu Chengji, Xunzhong Qi","doi":"10.1016/j.alcohol.2024.10.047","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>The high mobility group box 1 (HMGB1) pathway plays a pivotal role in the development of alcohol-induced brain injury. Glycyrrhizinic acid (GlyA) is widely regarded as an inhibitor of HMGB1. The objective is to investigate the impact on gene expression in the prefrontal cortex,we sequenced the transcriptome in control, alcohol-exposed, and HMGB1-inhibited groups of mice. We verified our findings by real-time quantitative PCR (qRT-PCR).</p><p><strong>Methods: </strong>An alcohol exposure model was established in mice by intraperitoneal injection of alcohol. Transcriptome sequencing and bioinformatics analyses were performed on prefrontal cortex tissue. Kyoto Encyclopedia of Genes and Genomes analysis was performed to identify pivotal pathways of differentially expressed genes. The role of relevant genes was verified by qRT-PCR.</p><p><strong>Results: </strong>Expression of genes involved in the neuroactive ligand-receptor interaction pathway exhibited an increase in mice from the alcohol-exposed group.However, there were no significant differences observed in the expression of these genes between control and those receiving an intraperitoneal injection of alcohol along with a HMGB1 inhibitor. Mice in the alcohol-exposed group showed increased gene expression of Cysltr2, Chrna6, Chrna3, Chrnb4, and Pmch. Expression of these genes was decreased in mice injected with HMGB1 inhibitor.</p><p><strong>Significance: </strong>Our study demonstrates that alcohol primarily influences gene expression in the prefrontal cortex of mice through the neuroactive ligand-receptor interaction pathway. HMGB1 inhibitor effectively inhibited the expression of this pathway. This study provides a novel route for drug development in alcohol-induced brain injury.</p>","PeriodicalId":93864,"journal":{"name":"Alcohol (Fayetteville, N.Y.)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Alcohol (Fayetteville, N.Y.)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.alcohol.2024.10.047","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Introduction: The high mobility group box 1 (HMGB1) pathway plays a pivotal role in the development of alcohol-induced brain injury. Glycyrrhizinic acid (GlyA) is widely regarded as an inhibitor of HMGB1. The objective is to investigate the impact on gene expression in the prefrontal cortex,we sequenced the transcriptome in control, alcohol-exposed, and HMGB1-inhibited groups of mice. We verified our findings by real-time quantitative PCR (qRT-PCR).

Methods: An alcohol exposure model was established in mice by intraperitoneal injection of alcohol. Transcriptome sequencing and bioinformatics analyses were performed on prefrontal cortex tissue. Kyoto Encyclopedia of Genes and Genomes analysis was performed to identify pivotal pathways of differentially expressed genes. The role of relevant genes was verified by qRT-PCR.

Results: Expression of genes involved in the neuroactive ligand-receptor interaction pathway exhibited an increase in mice from the alcohol-exposed group.However, there were no significant differences observed in the expression of these genes between control and those receiving an intraperitoneal injection of alcohol along with a HMGB1 inhibitor. Mice in the alcohol-exposed group showed increased gene expression of Cysltr2, Chrna6, Chrna3, Chrnb4, and Pmch. Expression of these genes was decreased in mice injected with HMGB1 inhibitor.

Significance: Our study demonstrates that alcohol primarily influences gene expression in the prefrontal cortex of mice through the neuroactive ligand-receptor interaction pathway. HMGB1 inhibitor effectively inhibited the expression of this pathway. This study provides a novel route for drug development in alcohol-induced brain injury.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
高迁移率组框 1 通路抑制对暴露于酒精的小鼠前额叶皮层基因表达的影响。
简介高迁移率基团框 1(HMGB1)通路在酒精诱发的脑损伤发展过程中起着关键作用。甘草酸(GlyA)被广泛认为是 HMGB1 的抑制剂。我们对对照组、酒精暴露组和 HMGB1 抑制组小鼠的转录组进行了测序。我们通过实时定量 PCR(qRT-PCR)验证了我们的研究结果:方法:通过腹腔注射酒精建立小鼠酒精暴露模型。对前额叶皮层组织进行了转录组测序和生物信息学分析。对京都基因和基因组百科全书进行了分析,以确定差异表达基因的关键通路。通过 qRT-PCR 验证了相关基因的作用:结果:参与神经活性配体-受体相互作用通路的基因在酒精暴露组小鼠中的表达量有所增加,但这些基因的表达量在对照组与腹腔注射酒精和 HMGB1 抑制剂的小鼠之间没有观察到显著差异。酒精暴露组小鼠的 Cysltr2、Chrna6、Chrna3、Chrnb4 和 Pmch 基因表达量增加。注射了 HMGB1 抑制剂的小鼠这些基因的表达量减少:我们的研究表明,酒精主要通过神经活性配体-受体相互作用途径影响小鼠前额叶皮层的基因表达。HMGB1抑制剂能有效抑制这一途径的表达。这项研究为开发治疗酒精所致脑损伤的药物提供了一条新途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Detecting the comorbidity of attention deficit hyperactivity disorder (ADHD) in a population of outpatients with alcohol use disorder (AUD): the role of personality traits, age at first alcohol use and level of craving. Effect of high mobility group box 1 pathway inhibition on gene expression in the prefrontal cortex of mice exposed to alcohol. Evaluating a 30-day alcohol abstinence challenge in heavy-drinking individuals with and without chronic pain: feasibility, safety, and perceived benefits. Consumption of oxycodone prevents oxytocin from attenuating alcohol intake in rats. Sex-dependent effects of ethanol withdrawal from a single- and repeated binge episode exposures on social anxiety-like behavior and neuropeptide gene expression in adolescent rats.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1