Biomimetic periosteum-bone scaffolds with codelivery of BMP-2 and PDGF-BB for skull repair

IF 3.5 2区 医学 Q2 ENDOCRINOLOGY & METABOLISM Bone Pub Date : 2024-11-04 DOI:10.1016/j.bone.2024.117315
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Abstract

Tissue engineering employs the use of bioactive materials to facilitate the filling and acceleration of bone defect healing, thereby introducing novel concepts to the field of in situ bone repair. Some studies have shown that periosteum plays an important role in bone regeneration and repair. In this study, biomimetic periosteum-bone scaffolds were prepared by depositing poly-L-lactic acid (PLLA) electrospun fibers on the surface of the gelatin/chitosan cryogel to mimic the bone and periosteum structure, respectively. To improve the bioactivity of the scaffold, bone morphogenetic protein-2 (BMP-2) was loaded into a loose porous mesh-like cryogel, while platelet-derived growth factor-BB (PDGF-BB) was encapsulated in the core of PLLA nanofibers with core-shell structure. Both of these two growth factors were released locally at the site of bone defect, where they exert a synergistic effect on osteogenesis, thereby greatly accelerating bone healing. The in vitro experiments demonstrated that the biomimetic periosteum-bone scaffolds exhibited favourable biocompatibility and osteogenesis ability. Furthermore, the in vivo experiments indicated that the composite scaffold repaired rat skull defects in a more rapid and effective manner. In conclusion, biomimetic periosteum-bone scaffolds with codelivery of BMP-2 and PDGF-BB shows significant potential for bone regeneration.
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生物仿真骨膜-骨支架与 BMP-2 和 PDGF-BB 共同输送用于颅骨修复。
组织工程学利用生物活性材料促进骨缺损的填充和加速愈合,从而为原位骨修复领域引入了新的概念。一些研究表明,骨膜在骨再生和修复中发挥着重要作用。本研究通过在明胶/壳聚糖低温凝胶表面沉积聚左旋乳酸(PLLA)电纺纤维,分别模拟骨和骨膜结构,制备了生物仿真骨膜-骨支架。为了提高支架的生物活性,骨形态发生蛋白-2(BMP-2)被载入疏松多孔的网状冷凝胶中,而血小板衍生生长因子-BB(PDGF-BB)则被包裹在具有核壳结构的 PLLA 纳米纤维的核心中。这两种生长因子在骨缺损部位局部释放,对骨生成产生协同作用,从而大大加速了骨愈合。体外实验表明,仿生骨膜-骨支架具有良好的生物相容性和成骨能力。此外,体内实验表明,复合支架能更快速、更有效地修复大鼠颅骨缺损。总之,共同递送 BMP-2 和 PDGF-BB 的仿生骨膜-骨支架在骨再生方面具有巨大潜力。
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来源期刊
Bone
Bone 医学-内分泌学与代谢
CiteScore
8.90
自引率
4.90%
发文量
264
审稿时长
30 days
期刊介绍: BONE is an interdisciplinary forum for the rapid publication of original articles and reviews on basic, translational, and clinical aspects of bone and mineral metabolism. The Journal also encourages submissions related to interactions of bone with other organ systems, including cartilage, endocrine, muscle, fat, neural, vascular, gastrointestinal, hematopoietic, and immune systems. Particular attention is placed on the application of experimental studies to clinical practice.
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