Unveiling contribution and fate of nitrogen with 15N techniques affected by microbial co-inoculation on field-grown maize: A novel approach to optimize N-fertilizer use efficiency
Fernando Shintate Galindo , Paulo Humberto Pagliari , Edson Cabral da Silva , Bruno Horschut de Lima , Guilherme Carlos Fernandes , Cassio Carlette Thiengo , João Victor Silva Bernardes , Arshad Jalal , Carlos Eduardo da Silva Oliveira , Gustavo Duprat dos Santos , Pedro Augusto Fedato Longato , Deyvison de Asevedo Soares , Lucila de Sousa Vilela , Reges Heinrichs , Marcelo Carvalho Minhoto Teixeira Filho , José Lavres
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引用次数: 0
Abstract
The objectives of this research were to: i) develop a mechanistic understanding of the synergy between microbial co-inoculation, nitrogen (N) fertilizer, and maize plants on biological 15N fixation, and 15N-recovery from applied fertilizers; and ii) explore the mechanist effects of microbial co-inoculation on N fractionations and derivation (fertilizer, atmosphere and soil), physiological responses on water use and carboxylation efficiencies and growth by using two different isotopic techniques under field conditions. Treatments included four seed inoculations (Control, B. subtilis, A. brasilense, and the combination of B. subtilis and A. brasilense), along with five levels of N application (0–240 kg N ha−1). Overall, the results indicate that maize co-inoculation with the above-mentioned bacteria enhanced photosynthetic efficiency leading to improved carboxylation efficiency and instantaneous water use efficiency in maize plants, likely due to an increase in net photosynthetic rate. This effect was more evident under low N availability. The findings also suggest that co-inoculation enhanced the ability of maize plants to absorb CO2, adjust to different soil N levels, and carry out photosynthesis, which resulted in higher carbon fixation and better maize growth. The N obtained from the atmosphere resulting from inoculation ranged from 25 to 50 kg N ha−1. Nonetheless, N application rates exceeding 186 kg N ha−1 substantially diminished the ability of these bacteria to fix N2. The combination of inoculation with the application of 120–180 kg N ha−1 led to a synergistic effect resulting in the greatest N-use efficiency, -recovery and grain yield.
期刊介绍:
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
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