Unveiling contribution and fate of nitrogen with 15N techniques affected by microbial co-inoculation on field-grown maize: A novel approach to optimize N-fertilizer use efficiency

IF 6.1 2区 生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2024-11-02 DOI:10.1016/j.plaphy.2024.109261
Fernando Shintate Galindo , Paulo Humberto Pagliari , Edson Cabral da Silva , Bruno Horschut de Lima , Guilherme Carlos Fernandes , Cassio Carlette Thiengo , João Victor Silva Bernardes , Arshad Jalal , Carlos Eduardo da Silva Oliveira , Gustavo Duprat dos Santos , Pedro Augusto Fedato Longato , Deyvison de Asevedo Soares , Lucila de Sousa Vilela , Reges Heinrichs , Marcelo Carvalho Minhoto Teixeira Filho , José Lavres
{"title":"Unveiling contribution and fate of nitrogen with 15N techniques affected by microbial co-inoculation on field-grown maize: A novel approach to optimize N-fertilizer use efficiency","authors":"Fernando Shintate Galindo ,&nbsp;Paulo Humberto Pagliari ,&nbsp;Edson Cabral da Silva ,&nbsp;Bruno Horschut de Lima ,&nbsp;Guilherme Carlos Fernandes ,&nbsp;Cassio Carlette Thiengo ,&nbsp;João Victor Silva Bernardes ,&nbsp;Arshad Jalal ,&nbsp;Carlos Eduardo da Silva Oliveira ,&nbsp;Gustavo Duprat dos Santos ,&nbsp;Pedro Augusto Fedato Longato ,&nbsp;Deyvison de Asevedo Soares ,&nbsp;Lucila de Sousa Vilela ,&nbsp;Reges Heinrichs ,&nbsp;Marcelo Carvalho Minhoto Teixeira Filho ,&nbsp;José Lavres","doi":"10.1016/j.plaphy.2024.109261","DOIUrl":null,"url":null,"abstract":"<div><div>The objectives of this research were to: i) develop a mechanistic understanding of the synergy between microbial co-inoculation, nitrogen (N) fertilizer, and maize plants on biological <sup>15</sup>N fixation, and <sup>15</sup>N-recovery from applied fertilizers; and ii) explore the mechanist effects of microbial co-inoculation on N fractionations and derivation (fertilizer, atmosphere and soil), physiological responses on water use and carboxylation efficiencies and growth by using two different isotopic techniques under field conditions. Treatments included four seed inoculations (Control, <em>B. subtilis</em>, <em>A. brasilense</em>, and the combination of <em>B. subtilis</em> and <em>A. brasilense</em>), along with five levels of N application (0–240 kg N ha<sup>−1</sup>). Overall, the results indicate that maize co-inoculation with the above-mentioned bacteria enhanced photosynthetic efficiency leading to improved carboxylation efficiency and instantaneous water use efficiency in maize plants, likely due to an increase in net photosynthetic rate. This effect was more evident under low N availability. The findings also suggest that co-inoculation enhanced the ability of maize plants to absorb CO<sub>2</sub>, adjust to different soil N levels, and carry out photosynthesis, which resulted in higher carbon fixation and better maize growth. The N obtained from the atmosphere resulting from inoculation ranged from 25 to 50 kg N ha<sup>−1</sup>. Nonetheless, N application rates exceeding 186 kg N ha<sup>−1</sup> substantially diminished the ability of these bacteria to fix N<sub>2</sub>. The combination of inoculation with the application of 120–180 kg N ha<sup>−1</sup> led to a synergistic effect resulting in the greatest N-use efficiency, -recovery and grain yield.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109261"},"PeriodicalIF":6.1000,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S098194282400929X","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

The objectives of this research were to: i) develop a mechanistic understanding of the synergy between microbial co-inoculation, nitrogen (N) fertilizer, and maize plants on biological 15N fixation, and 15N-recovery from applied fertilizers; and ii) explore the mechanist effects of microbial co-inoculation on N fractionations and derivation (fertilizer, atmosphere and soil), physiological responses on water use and carboxylation efficiencies and growth by using two different isotopic techniques under field conditions. Treatments included four seed inoculations (Control, B. subtilis, A. brasilense, and the combination of B. subtilis and A. brasilense), along with five levels of N application (0–240 kg N ha−1). Overall, the results indicate that maize co-inoculation with the above-mentioned bacteria enhanced photosynthetic efficiency leading to improved carboxylation efficiency and instantaneous water use efficiency in maize plants, likely due to an increase in net photosynthetic rate. This effect was more evident under low N availability. The findings also suggest that co-inoculation enhanced the ability of maize plants to absorb CO2, adjust to different soil N levels, and carry out photosynthesis, which resulted in higher carbon fixation and better maize growth. The N obtained from the atmosphere resulting from inoculation ranged from 25 to 50 kg N ha−1. Nonetheless, N application rates exceeding 186 kg N ha−1 substantially diminished the ability of these bacteria to fix N2. The combination of inoculation with the application of 120–180 kg N ha−1 led to a synergistic effect resulting in the greatest N-use efficiency, -recovery and grain yield.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
利用 15N 技术揭示受田间种植玉米上微生物共同接种影响的氮的贡献和归宿:优化氮肥利用效率的新方法
本研究的目标是:i) 从机理上理解微生物联合接种、氮(N)肥料和玉米植物对生物 15N 固定和从施肥中回收 15N 的协同作用;ii) 在田间条件下,利用两种不同的同位素技术,探索微生物联合接种对氮的分馏和衍生(肥料、大气和土壤)、对水分利用和羧化效率的生理反应以及生长的机理影响。处理包括四种种子接种(对照、枯草芽孢杆菌、巴西芽孢杆菌以及枯草芽孢杆菌和巴西芽孢杆菌的组合),以及五种水平的氮施用量(0-240 千克氮公顷-1)。总之,研究结果表明,玉米与上述细菌共同接种可提高光合效率,从而改善玉米植株的羧化效率和瞬时水分利用效率,这可能是由于净光合速率的提高。在氮供应量低的情况下,这种效果更为明显。研究结果还表明,联合接种增强了玉米植株吸收二氧化碳、适应不同土壤氮水平和进行光合作用的能力,从而提高了碳固定量,改善了玉米的生长状况。接种后从大气中获得的氮在 25 至 50 千克/公顷之间。然而,氮的施用量超过每公顷 186 千克氮时,这些细菌固定 N2 的能力就会大大降低。将接种与施用 120-180 千克氮(每公顷)结合起来可产生协同效应,从而获得最高的氮利用效率、氮回收率和谷物产量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Plant Physiology and Biochemistry
Plant Physiology and Biochemistry 生物-植物科学
CiteScore
11.10
自引率
3.10%
发文量
410
审稿时长
33 days
期刊介绍: Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
期刊最新文献
Functional characterization of Camptotheca acuminata 7-deoxyloganetic acid synthases and 7-deoxyloganetic acid glucosyltransferases involved in camptothecin biosynthesis. Developing fluorescence hyperspectral imaging methods for non-invasive detection of herbicide safeners action mechanism and effectiveness. Effect of elevated ammonium on biotic and abiotic stress defense responses and expression of related genes in cucumber (Cucumis sativus L.) plants. Genome-wide identification of SWEET gene family and the sugar transport function of three candidate genes during female flower bud induction stage of Juglans sigillata Dode. ZnO nanoparticles enhances cadmium tolerance by modulating N6-methyladenosine (m6A) level of stress-responsive genes NRT1 and GM35E in vegetable soybean.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1