Engineering modular and tunable single-molecule sensors by decoupling sensing from signal output

IF 38.1 1区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY Nature nanotechnology Pub Date : 2024-11-07 DOI:10.1038/s41565-024-01804-0

Lennart Grabenhorst, Martina Pfeiffer, Thea Schinkel, Mirjam Kümmerlin, Gereon A. Brüggenthies, Jasmin B. Maglic, Florian Selbach, Alexander T. Murr, Philip Tinnefeld, Viktorija Glembockyte

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Abstract

Biosensors play key roles in medical research and diagnostics. However, the development of biosensors for new biomolecular targets of interest often involves tedious optimization steps to ensure a high signal response at the analyte concentration of interest. Here we show a modular nanosensor platform that facilitates these steps by offering ways to decouple and independently tune the signal output as well as the response window. Our approach utilizes a dynamic DNA origami nanostructure to engineer a high optical signal response based on fluorescence resonance energy transfer. We demonstrate mechanisms to tune the sensor’s response window, specificity and cooperativity as well as highlight the modularity of the proposed platform by extending it to different biomolecular targets including more complex sensing schemes. This versatile nanosensor platform offers a promising starting point for the rapid development of biosensors with tailored properties.

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通过将传感与信号输出解耦，设计模块化可调谐单分子传感器

生物传感器在医学研究和诊断中发挥着关键作用。然而，针对新的生物分子目标开发生物传感器往往涉及繁琐的优化步骤，以确保在相关分析物浓度下的高信号响应。在这里，我们展示了一种模块化纳米传感器平台，它通过提供解耦和独立调节信号输出以及响应窗口的方法来简化这些步骤。我们的方法利用动态 DNA 折纸纳米结构，在荧光共振能量转移的基础上设计出高光学信号响应。我们展示了调整传感器响应窗口、特异性和合作性的机制，并通过将其扩展到不同的生物分子目标（包括更复杂的传感方案）来突出所提议平台的模块性。这种多功能纳米传感器平台为快速开发具有定制特性的生物传感器提供了一个很好的起点。

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来源期刊

Nature nanotechnology 工程技术-材料科学：综合

CiteScore

59.70

自引率

0.80%

发文量

196

审稿时长

4-8 weeks

期刊介绍： Nature Nanotechnology is a prestigious journal that publishes high-quality papers in various areas of nanoscience and nanotechnology. The journal focuses on the design, characterization, and production of structures, devices, and systems that manipulate and control materials at atomic, molecular, and macromolecular scales. It encompasses both bottom-up and top-down approaches, as well as their combinations. Furthermore, Nature Nanotechnology fosters the exchange of ideas among researchers from diverse disciplines such as chemistry, physics, material science, biomedical research, engineering, and more. It promotes collaboration at the forefront of this multidisciplinary field. The journal covers a wide range of topics, from fundamental research in physics, chemistry, and biology, including computational work and simulations, to the development of innovative devices and technologies for various industrial sectors such as information technology, medicine, manufacturing, high-performance materials, energy, and environmental technologies. It includes coverage of organic, inorganic, and hybrid materials.