Identification of cambium stem cell factors and their positioning mechanism

IF 44.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Science Pub Date : 2024-11-07 DOI:10.1126/science.adj8752

Gugan Eswaran, Xixi Zhang, Jacob Pieter Rutten, Jingyi Han, Hiroyuki Iida, Jennifer López Ortiz, Riikka Mäkilä, Brecht Wybouw, Benjamin Planterose Jiménez, Leo Vainio, Alexis Porcher, Marina Leal Gavarron, Jing Zhang, Tiina Blomster, Xin Wang, David Dolan, Ondřej Smetana, Siobhán M. Brady, Melis Kucukoglu Topcu, Kirsten ten Tusscher, J. Peter Etchells, Ari Pekka Mähönen

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Abstract

Wood constitutes the largest reservoir of terrestrial biomass. Composed of xylem, it arises from one side of the vascular cambium, a bifacial stem cell niche that also produces phloem on the opposing side. It is currently unknown which molecular factors endow cambium stem cell identity. Here we show that TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF) ligand–activated PHLOEM INTERCALATED WITH XYLEM (PXY) receptors promote the expression of CAMBIUM-EXPRESSED AINTEGUMENTA-LIKE (CAIL) transcription factors to define cambium stem cell identity in the Arabidopsis root. By sequestrating the phloem-originated TDIF, xylem-expressed PXY confines the TDIF signaling front, resulting in the activation of CAIL expression and stem cell identity in only a narrow domain. Our findings show how signals emanating from cells on opposing sides ensure robust yet dynamically adjustable positioning of a bifacial stem cell layer.

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鉴定骨干网干细胞因子及其定位机制

木材是陆地生物量的最大储存库。木材由木质部组成，来自维管凸起的一侧，这是一个双面干细胞龛，在对立面也产生韧皮部。目前还不知道哪些分子因子赋予了韧皮部干细胞的特性。在这里，我们展示了TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF)配体激活的PHLOEM INTERCALATED WITH XYLEM (PXY)受体可促进CAMBIUM-EXPRESSED AINTEGUMENTA-LIKE(CAIL)转录因子的表达，从而确定拟南芥根部的韧皮部干细胞特征。木质部表达的 PXY 通过封存韧皮部起源的 TDIF，限制了 TDIF 信号转导前沿，导致 CAIL 的表达被激活，干细胞特征只在一个狭窄的区域内表达。我们的研究结果表明，来自对侧细胞的信号如何确保双面干细胞层的稳健、动态可调定位。

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

期刊介绍： Science is a leading outlet for scientific news, commentary, and cutting-edge research. Through its print and online incarnations, Science reaches an estimated worldwide readership of more than one million. Science’s authorship is global too, and its articles consistently rank among the world's most cited research. Science serves as a forum for discussion of important issues related to the advancement of science by publishing material on which a consensus has been reached as well as including the presentation of minority or conflicting points of view. Accordingly, all articles published in Science—including editorials, news and comment, and book reviews—are signed and reflect the individual views of the authors and not official points of view adopted by AAAS or the institutions with which the authors are affiliated. Science seeks to publish those papers that are most influential in their fields or across fields and that will significantly advance scientific understanding. Selected papers should present novel and broadly important data, syntheses, or concepts. They should merit recognition by the wider scientific community and general public provided by publication in Science, beyond that provided by specialty journals. Science welcomes submissions from all fields of science and from any source. The editors are committed to the prompt evaluation and publication of submitted papers while upholding high standards that support reproducibility of published research. Science is published weekly; selected papers are published online ahead of print.