SNPs in the Galectin-8 gene of Acanthopagrus latus have been found to be associated with resistance trait to Streptococcus iniae

IF 2.2 3区农林科学 Q2 FISHERIES Aquaculture International Pub Date : 2024-11-08 DOI:10.1007/s10499-024-01710-1

Jin-Min Pan, Hua-Yang Guo, Bao-Suo Liu, Nan Zhang, Lin Xian, Teng-Fei Zhu, Ke-Cheng Zhu, Dian-Chang Zhang

{"title":"SNPs in the Galectin-8 gene of Acanthopagrus latus have been found to be associated with resistance trait to Streptococcus iniae","authors":"Jin-Min Pan, Hua-Yang Guo, Bao-Suo Liu, Nan Zhang, Lin Xian, Teng-Fei Zhu, Ke-Cheng Zhu, Dian-Chang Zhang","doi":"10.1007/s10499-024-01710-1","DOIUrl":null,"url":null,"abstract":"<div>Seabream is one of most important candidates for aquaculture in China. However, in recent years, streptococcosis has posed a serious challenge. Galectin-8 has been proven to play an important role in the antibacterial immunity of teleost. In this study, Acanthopagrus latus Galectin-8 (AlGal-8) was characterized for the first time consisting of 8 exons and 7 introns. AlGal-8 protein was considered to dock with N-Acetylglucosamine in the CRD region. In healthy tissues, AlGal-8 was found to be expressed highest in the gills, followed by brain, kidney and liver. After infection with Streptococcus iniae, the expression of AlGal-8 in the brain, kidney, and liver increased to varying degrees, but there was no significant change in the spleen. We also analyzed SNPs present in the genomic region of AlGal-8, and a total of 8 SNPs were found. One SNPs in exon (G2264C) and two in introns (T4799G and C4892T) were significantly (P < 0.05) associated with resistance to S. iniae. This study not only provides a reference for the role of Galectin-8 in the antibacterial immunity of teleost but also provides fundamental information for the disease-resistant molecular breeding of A. latus.</div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture International","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s10499-024-01710-1","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 0

Abstract

Seabream is one of most important candidates for aquaculture in China. However, in recent years, streptococcosis has posed a serious challenge. Galectin-8 has been proven to play an important role in the antibacterial immunity of teleost. In this study, Acanthopagrus latus Galectin-8 (AlGal-8) was characterized for the first time consisting of 8 exons and 7 introns. AlGal-8 protein was considered to dock with N-Acetylglucosamine in the CRD region. In healthy tissues, AlGal-8 was found to be expressed highest in the gills, followed by brain, kidney and liver. After infection with Streptococcus iniae, the expression of AlGal-8 in the brain, kidney, and liver increased to varying degrees, but there was no significant change in the spleen. We also analyzed SNPs present in the genomic region of AlGal-8, and a total of 8 SNPs were found. One SNPs in exon (G2264C) and two in introns (T4799G and C4892T) were significantly (P < 0.05) associated with resistance to S. iniae. This study not only provides a reference for the role of Galectin-8 in the antibacterial immunity of teleost but also provides fundamental information for the disease-resistant molecular breeding of A. latus.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

发现花斑裸鲤 Galectin-8 基因中的 SNPs 与对猪链球菌的抗性特征有关

鲷鱼是中国最重要的水产养殖对象之一。然而，近年来链球菌病给养殖带来了严峻的挑战。Galectin-8已被证实在远洋鱼类的抗菌免疫中发挥重要作用。本研究首次鉴定了宽吻鲈的Galectin-8（AlGal-8），该蛋白由8个外显子和7个内含子组成。AlGal-8 蛋白被认为能与 CRD 区域的 N-乙酰葡糖胺对接。在健康组织中，AlGal-8 在鳃中的表达量最高，其次是脑、肾和肝。感染猪链球菌后，AlGal-8在大脑、肾脏和肝脏中的表达量有不同程度的增加，但在脾脏中没有明显变化。我们还分析了 AlGal-8 基因组中的 SNPs，共发现 8 个 SNPs。其中一个位于外显子（G2264C）、两个位于内含子（T4799G 和 C4892T）的 SNPs 与对 S. iniae 的抗性显著相关（P < 0.05）。该研究不仅为Galectin-8在长尾鳕抗菌免疫中的作用提供了参考，也为长尾鳕抗病分子育种提供了基础信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.