Insights into larval development and protein biochemical alterations of Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae) following Beauveria bassiana and Solanum lycopersicum treatments

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Chemical and Biological Technologies in Agriculture Pub Date : 2024-11-07 DOI:10.1186/s40538-024-00692-9

Ghada E. Abd-Allah, Moataz A. M. Moustafa, Fatma S. Ahmed, Eman El-said, Enayat M. Elqady, Lina A. Abou El-Khashab, Hend H. A. Salem

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Abstract

Background

The polyphagous notorious pest, black cutworm, Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae), cause significant production losses due to its distinctive feeding and hiding behavior, making it particularly challenging to control it with conventional methods. Therefore, sustainable agriculture demands more effective and environmentally safe pest control solutions. This study aimed to investigate the toxicity of two insecticide alternatives, the entomopathogenic fungus (EPF) Beauveria bassiana and Solanum lycopersicum extract (Tomato plant crude extract, TPCE), using two bioassay methods: the poisoned bait method and the leaf dipping method. In addition, the impact of these biological tools on larval development and protein profiles was evaluated.

Results

The bait application of both tested materials exhibited higher toxicity than the leaf dipping method, as indicated by the toxicity index. The LC₅₀ values for B. bassiana were 1.6 × 10⁸ and 1.8 × 10⁶ conidia ml⁻¹ using the leaf dipping method and poisoned baits method, respectively. For TPCE, the LC₅₀ values were 4.35 and 1.51 mg ml⁻¹ for the same methods, respectively. In addition, sublethal concentrations of both materials altered the larval and pupal durations. B. bassiana significantly reduced the concentration of larval hemolymph protein. A maximum of 12 protein bands in the control sample, with molecular weights (Mw) ranging between 35 and 120 kDa, were detected by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). In B. bassiana-treated larvae, ten bands were detected with Mw ranging from 35 to 120 kDa. At least seven bands were detected in TPCE-treated larvae, with Mw ranging from 35 to 97 kDa.

Conclusions

The findings of this study can be integrated into management programs for A. ipsilon. In addition, the availability of B. bassiana and TPCE in Egypt and their cost-effectiveness as insecticide alternatives support their use in the management programs of this critical pest. These methods are particularly effective when applied in bait form.

Graphical Abstract

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Beauveria bassiana 和 Solanum lycopersicum 处理 Agrotis ipsilon (Hufnagel) （鳞翅目：夜蛾科）后幼虫发育和蛋白质生化变化的启示

背景臭名昭著的多食性害虫--黑刺虫（Agrotis ipsilon (Hufnagel)）（鳞翅目：夜蛾科），由于其独特的取食和藏匿行为，会给生产造成重大损失，因此用传统方法控制这种害虫特别具有挑战性。因此，可持续农业需要更有效和环境安全的害虫控制解决方案。本研究旨在使用两种生物测定方法（毒饵法和浸叶法）研究两种杀虫剂替代品的毒性，即昆虫病原真菌（EPF）Beauveria bassiana 和番茄提取物（番茄植物粗提取物，TPCE）。此外，还评估了这些生物工具对幼虫发育和蛋白质特征的影响。结果从毒性指数来看，施用两种受试材料的毒饵比浸叶法毒性更高。浸叶法和毒饵法对 B. bassiana 的 LC50 值分别为 1.6 × 10⁸ 和 1.8 × 10⁶ 分生孢子 ml-1。对于 TPCE，相同方法的 LC50 值分别为 4.35 毫克毫升-1 和 1.51 毫克毫升-1。此外，两种材料的亚致死浓度都会改变幼虫和蛹的持续时间。B. bassiana 能明显降低幼虫血淋巴蛋白的浓度。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）在对照样本中最多检测到 12 条蛋白质条带，分子量（Mw）介于 35 和 120 kDa 之间。在 B. bassiana 处理过的幼虫中，检测到 10 条 Mw 在 35 至 120 kDa 之间的条带。在 TPCE 处理过的幼虫中至少检测到 7 条带，分子量在 35 到 97 kDa 之间。此外，B. bassiana 和 TPCE 在埃及的可用性及其作为杀虫剂替代品的成本效益也支持将它们用于这种关键害虫的管理计划中。这些方法在以诱饵形式施用时尤其有效。

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.