Highly specific detection of ROR1 cancer biomarker with bipolar electrochemiluminescence

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2024-11-08 DOI:10.1007/s00604-024-06815-1

Seyed Mohammad Reza Mortazavi, Morteza Hosseini, Guobao Xu, Hossein Naderi-Manesh, Mohammad Reza Ganjali

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Abstract

An electrochemiluminescence (ECL) detection system is presented integrated with a bipolar electrode system for sensitive cancer diagnosis. In order to achieve the highest electrical conductivity and redox-active surface area, MXene was chosen as the material for the bipolar electrode. As part of the detection process, the anodic pole of the bipolar electrode was modified with the receptor tyrosine kinase like orphan receptor 1 (ROR1) antibody, followed by an immunoassay using the ROR1 antibody-modified Ag triangle that was identified as significantly enhancing ECL. We measured the ECL of luminol using the anode pole of BPE as an analytical signal in the presence of H₂O₂. Additionally, 3D-printed microchannels were used to fabricate the BPE system, to reduce the quantity of sample required. It has been shown that the present immunosensors are low-cost and sensitive in detecting types of cancer, with an extended linear range of 10 fg mL⁻¹ to 1 µg mL⁻¹ in the analysis of synthetic samples and achieving an accuracy of ~ 90% in diagnosing ten unknown real samples.

Graphical Abstract

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利用双极电化学发光技术高度特异性地检测 ROR1 癌症生物标记物

本文介绍了一种与双极电极系统集成的电化学发光（ECL）检测系统，用于灵敏的癌症诊断。为了获得最高的导电性和氧化还原活性表面积，双极电极选择了 MXene 作为材料。作为检测过程的一部分，双极电极的阳极用受体酪氨酸激酶类孤儿受体 1（ROR1）抗体修饰，然后使用 ROR1 抗体修饰的 Ag 三角形进行免疫测定，结果表明该抗体可显著增强 ECL。在 H2O2 存在的情况下，我们使用 BPE 的阳极作为分析信号测量了发光酚的 ECL。此外，我们还使用 3D 打印微通道来制造 BPE 系统，以减少所需的样品量。研究表明，这种免疫传感器成本低，对癌症类型的检测灵敏度高，在分析合成样品时，线性范围从 10 fg mL-1 扩展到 1 µg mL-1，在诊断十种未知的真实样品时，准确率达到约 90%。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.