Development of an HPPD-Inhibitor Resistant Wheat and Multiomics Integrative Analysis of Herbicide Toxicity and OsHIS1 Detoxification in Wheat

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of Agricultural and Food Chemistry Pub Date : 2024-11-08 DOI:10.1021/acs.jafc.4c07783

Chenxu Zhao, Luhui Wang, Gaohui Qu, Liya Zhu, Zihan Liu, Pingchuang Deng, Jixin Zhao, Changyou Wang, Chunhuan Chen, Wanquan Ji, Tingdong Li

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Abstract

Weed infestation in agricultural fields significantly diminishes crop yields. Herbicides are widely used as a primary method of weed control. Developing herbicide-resistant crops through the expression of resistant genes represents a sustainable approach. This study generated wheat germplasms highly resistant to 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides by transforming the rice HPPD INHIBITOR SENSITIVE 1 (OsHIS1) gene into Xinong 511, conferring resistance to mesotrione at levels up to nine times the typical field application rate (1350 g ai ha^–1). Agronomic trait evaluations under greenhouse and field conditions showed no additional effects on wheat. Herbicide susceptibility assays confirmed the specific resistance to different HPPD inhibitors. Transcriptome and metabolome analyses revealed regulation of flavonoid and photosynthesis-antenna protein pathways in the herbicide functional. Collectively, OsHIS1 could be applied in the production of herbicide-resistant wheat.

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抗 HPPD 抑制剂小麦的开发以及小麦除草剂毒性和 OsHIS1 解毒的多组学综合分析

农田杂草丛生会大大降低作物产量。除草剂被广泛用作控制杂草的主要方法。通过表达抗性基因来培育抗除草剂作物是一种可持续的方法。本研究通过将水稻 HPPD INHIBITOR SENSITIVE 1（OsHIS1）基因转化到西农 511 中，培育出了对 4-羟基苯基丙酮酸二氧酶（HPPD）抑制性除草剂具有高度抗性的小麦种质，使其对介草酮的抗性水平达到典型田间施用量（1350 g ai ha-1）的 9 倍。在温室和田间条件下进行的农艺性状评估显示，该基因对小麦没有额外影响。除草剂敏感性测定证实了小麦对不同的 HPPD 抑制剂具有特异性抗性。转录组和代谢组分析表明，在除草剂的作用下，类黄酮和光合作用-天线蛋白途径受到调控。总之，OsHIS1可用于生产抗除草剂小麦。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.