{"title":"Stepwise Assembly of DNA Nanostructures in a Surface-Based Method","authors":"Huangchen Cui, Yaqi Wang, Linfeng Yang, Yifan Li, Yangtian Yu, Yu Miao, Tanxi Bai, Haiyue Wang, Tianqing Zhang, Jizhou Li, Jianbin Wang, Bryan Wei","doi":"10.1021/acsnano.4c06024","DOIUrl":null,"url":null,"abstract":"Hierarchical assembly of DNA nanostructures has already led to superstructures of ever-increasing level of complexity. Processing control in building nanostructures hierarchically is desirable but remains underexplored. Here, we present the stepwise assembly of DNA origami nanostructures by a surface-based method. With solid support of magnetic beads or glass slides, we demonstrate hierarchical assembly of preformed DNA origami units to a number of superstructures. The anchoring of DNA constructs on the surface results in better programmability and controllability for DNA self-assembly, suggesting a potential for our surface-based strategy to become a general and standardized assembly methodology of DNA nanostructures and beyond.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":null,"pages":null},"PeriodicalIF":15.8000,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Nano","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1021/acsnano.4c06024","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Hierarchical assembly of DNA nanostructures has already led to superstructures of ever-increasing level of complexity. Processing control in building nanostructures hierarchically is desirable but remains underexplored. Here, we present the stepwise assembly of DNA origami nanostructures by a surface-based method. With solid support of magnetic beads or glass slides, we demonstrate hierarchical assembly of preformed DNA origami units to a number of superstructures. The anchoring of DNA constructs on the surface results in better programmability and controllability for DNA self-assembly, suggesting a potential for our surface-based strategy to become a general and standardized assembly methodology of DNA nanostructures and beyond.
DNA 纳米结构的分层组装已经产生了复杂程度越来越高的超级结构。分层构建纳米结构的过程控制是可取的，但仍未得到充分探索。在这里，我们介绍了一种基于表面的 DNA 折纸纳米结构分步组装方法。在磁珠或玻璃载玻片的固体支撑下，我们展示了将预成型的 DNA 折纸单元分层组装成多种超结构的方法。将 DNA 构建物锚定在表面上能更好地实现 DNA 自组装的可编程性和可控性，这表明我们基于表面的策略有可能成为一种通用的、标准化的 DNA 纳米结构组装方法。
期刊介绍：
ACS Nano, published monthly, serves as an international forum for comprehensive articles on nanoscience and nanotechnology research at the intersections of chemistry, biology, materials science, physics, and engineering. The journal fosters communication among scientists in these communities, facilitating collaboration, new research opportunities, and advancements through discoveries. ACS Nano covers synthesis, assembly, characterization, theory, and simulation of nanostructures, nanobiotechnology, nanofabrication, methods and tools for nanoscience and nanotechnology, and self- and directed-assembly. Alongside original research articles, it offers thorough reviews, perspectives on cutting-edge research, and discussions envisioning the future of nanoscience and nanotechnology.
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