{"title":"Conjugated Polymer-Boosted Near-infrared Electrochemiluminescence of Organic Dye for Detecting Acetamiprid","authors":"Xin Wen, Ying He, Ruo Yuan, Shihong Chen","doi":"10.1016/j.aca.2024.343417","DOIUrl":null,"url":null,"abstract":"<h3>Background</h3>The near-infrared electrochemiluminescence (NIR-ECL) has excellent penetration and near zero background interference, and has shown unique advantages in clinical medicine and bioimaging. Among various types of NIR-ECL emitters, NIR organic dyes have arouse the concern of researchers due to their adjustable structure and diverse optical properties. However, the currently available NIR dyes usually have inherent self-quenching effect and poor photostability, so their ECL efficiency is low, and it is a great challenge to improve their ECL performance.<h3>Result</h3>Conjugated polymer-boosted NIR-ECL strategy was creatively developed to overcome ECL performance limitations of NIR dyes. IR 783, as one of heptamethine cyanine dyes, was performed a nanoprecipitation in the presence of poly[(9,9-dlhexyfluoren-2,7-dlyl)-co-(anthracen-9,10-dlyl)] (PFAD) to prepare IR polymer nanoparticles (IR PNPs). Due to resonance energy transfer (RET) from PFAD to IR 783 and encapsulation of IR 783 by PFAD, the resulting IR PNPs exhibited a strong and stable NIR-ECL emission with a maximum ECL wavelength of 802 nm under coreactant tripropylamine (TPrA) and H<sub>2</sub>O<sub>2</sub> can effectively quench it. IR PNPs coupled proximity ligation assay (PLA)-induced DNA walker to achieve acetamiprid (ACE) analysis. ACE triggered PLA to form bipedal DNA walker, and further release G-rich secondary target (ST). With ST and hemin being captured on IR PNPs modified electrode, hemin/G-quadruplex was assembled to consume H<sub>2</sub>O<sub>2</sub>, thereby restoring ECL signal for ACE detection with a limit of detection of 4.74 ×10<sup>-15</sup> M.<h3>Significance</h3>This work opens up a new and simple way to boost NIR-ECL of organic dyes, and IR PNPs create a promising NIR-ECL platform for pesticide detection.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":null,"pages":null},"PeriodicalIF":5.7000,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytica Chimica Acta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.aca.2024.343417","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Background
The near-infrared electrochemiluminescence (NIR-ECL) has excellent penetration and near zero background interference, and has shown unique advantages in clinical medicine and bioimaging. Among various types of NIR-ECL emitters, NIR organic dyes have arouse the concern of researchers due to their adjustable structure and diverse optical properties. However, the currently available NIR dyes usually have inherent self-quenching effect and poor photostability, so their ECL efficiency is low, and it is a great challenge to improve their ECL performance.
Result
Conjugated polymer-boosted NIR-ECL strategy was creatively developed to overcome ECL performance limitations of NIR dyes. IR 783, as one of heptamethine cyanine dyes, was performed a nanoprecipitation in the presence of poly[(9,9-dlhexyfluoren-2,7-dlyl)-co-(anthracen-9,10-dlyl)] (PFAD) to prepare IR polymer nanoparticles (IR PNPs). Due to resonance energy transfer (RET) from PFAD to IR 783 and encapsulation of IR 783 by PFAD, the resulting IR PNPs exhibited a strong and stable NIR-ECL emission with a maximum ECL wavelength of 802 nm under coreactant tripropylamine (TPrA) and H2O2 can effectively quench it. IR PNPs coupled proximity ligation assay (PLA)-induced DNA walker to achieve acetamiprid (ACE) analysis. ACE triggered PLA to form bipedal DNA walker, and further release G-rich secondary target (ST). With ST and hemin being captured on IR PNPs modified electrode, hemin/G-quadruplex was assembled to consume H2O2, thereby restoring ECL signal for ACE detection with a limit of detection of 4.74 ×10-15 M.
Significance
This work opens up a new and simple way to boost NIR-ECL of organic dyes, and IR PNPs create a promising NIR-ECL platform for pesticide detection.
期刊介绍:
Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review.
Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.