Conjugated Polymer-Boosted Near-infrared Electrochemiluminescence of Organic Dye for Detecting Acetamiprid

IF 5.7 2区 化学 Q1 CHEMISTRY, ANALYTICAL Analytica Chimica Acta Pub Date : 2024-11-13 DOI:10.1016/j.aca.2024.343417
Xin Wen, Ying He, Ruo Yuan, Shihong Chen
{"title":"Conjugated Polymer-Boosted Near-infrared Electrochemiluminescence of Organic Dye for Detecting Acetamiprid","authors":"Xin Wen, Ying He, Ruo Yuan, Shihong Chen","doi":"10.1016/j.aca.2024.343417","DOIUrl":null,"url":null,"abstract":"<h3>Background</h3>The near-infrared electrochemiluminescence (NIR-ECL) has excellent penetration and near zero background interference, and has shown unique advantages in clinical medicine and bioimaging. Among various types of NIR-ECL emitters, NIR organic dyes have arouse the concern of researchers due to their adjustable structure and diverse optical properties. However, the currently available NIR dyes usually have inherent self-quenching effect and poor photostability, so their ECL efficiency is low, and it is a great challenge to improve their ECL performance.<h3>Result</h3>Conjugated polymer-boosted NIR-ECL strategy was creatively developed to overcome ECL performance limitations of NIR dyes. IR 783, as one of heptamethine cyanine dyes, was performed a nanoprecipitation in the presence of poly[(9,9-dlhexyfluoren-2,7-dlyl)-co-(anthracen-9,10-dlyl)] (PFAD) to prepare IR polymer nanoparticles (IR PNPs). Due to resonance energy transfer (RET) from PFAD to IR 783 and encapsulation of IR 783 by PFAD, the resulting IR PNPs exhibited a strong and stable NIR-ECL emission with a maximum ECL wavelength of 802 nm under coreactant tripropylamine (TPrA) and H<sub>2</sub>O<sub>2</sub> can effectively quench it. IR PNPs coupled proximity ligation assay (PLA)-induced DNA walker to achieve acetamiprid (ACE) analysis. ACE triggered PLA to form bipedal DNA walker, and further release G-rich secondary target (ST). With ST and hemin being captured on IR PNPs modified electrode, hemin/G-quadruplex was assembled to consume H<sub>2</sub>O<sub>2</sub>, thereby restoring ECL signal for ACE detection with a limit of detection of 4.74 ×10<sup>-15</sup> M.<h3>Significance</h3>This work opens up a new and simple way to boost NIR-ECL of organic dyes, and IR PNPs create a promising NIR-ECL platform for pesticide detection.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":null,"pages":null},"PeriodicalIF":5.7000,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytica Chimica Acta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.aca.2024.343417","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Background

The near-infrared electrochemiluminescence (NIR-ECL) has excellent penetration and near zero background interference, and has shown unique advantages in clinical medicine and bioimaging. Among various types of NIR-ECL emitters, NIR organic dyes have arouse the concern of researchers due to their adjustable structure and diverse optical properties. However, the currently available NIR dyes usually have inherent self-quenching effect and poor photostability, so their ECL efficiency is low, and it is a great challenge to improve their ECL performance.

Result

Conjugated polymer-boosted NIR-ECL strategy was creatively developed to overcome ECL performance limitations of NIR dyes. IR 783, as one of heptamethine cyanine dyes, was performed a nanoprecipitation in the presence of poly[(9,9-dlhexyfluoren-2,7-dlyl)-co-(anthracen-9,10-dlyl)] (PFAD) to prepare IR polymer nanoparticles (IR PNPs). Due to resonance energy transfer (RET) from PFAD to IR 783 and encapsulation of IR 783 by PFAD, the resulting IR PNPs exhibited a strong and stable NIR-ECL emission with a maximum ECL wavelength of 802 nm under coreactant tripropylamine (TPrA) and H2O2 can effectively quench it. IR PNPs coupled proximity ligation assay (PLA)-induced DNA walker to achieve acetamiprid (ACE) analysis. ACE triggered PLA to form bipedal DNA walker, and further release G-rich secondary target (ST). With ST and hemin being captured on IR PNPs modified electrode, hemin/G-quadruplex was assembled to consume H2O2, thereby restoring ECL signal for ACE detection with a limit of detection of 4.74 ×10-15 M.

Significance

This work opens up a new and simple way to boost NIR-ECL of organic dyes, and IR PNPs create a promising NIR-ECL platform for pesticide detection.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
共轭聚合物增强型有机染料近红外电化学发光法检测啶虫脒
背景近红外电致化学发光(NIR-ECL)具有良好的穿透性和近乎零的背景干扰,在临床医学和生物成像中显示出独特的优势。在各类近红外电致发光发射体中,近红外有机染料因其结构可调、光学性质多样而备受研究人员关注。然而,现有的近红外染料通常具有固有的自淬效应和较差的光稳定性,因此其 ECL 效率较低,如何提高其 ECL 性能是一个巨大的挑战。在聚[(9,9-dlhexyfluoren-2,7-dlyl)-co-(anthracen-9,10-dlyl)](PFAD)存在下进行纳米沉淀,制备出红外聚合物纳米粒子(IR PNPs)。由于 PFAD 与 IR 783 之间的共振能量转移(RET)以及 PFAD 对 IR 783 的包裹,所制备的 IR PNPs 在核心反应物三丙胺(TPrA)和 H2O2 的有效淬灭下,表现出强烈而稳定的近红外-ECL 发射,最大 ECL 波长为 802 nm。红外 PNPs 与近距离连接试验(PLA)诱导的 DNA 步行器耦合,实现了啶虫脒(ACE)分析。ACE 触发 PLA 形成双足 DNA 步行器,并进一步释放富含 G 的二级靶标(ST)。随着 ST 和 hemin 被捕获到 IR PNPs 修饰的电极上,hemin/G-四联体被组装起来消耗 H2O2,从而恢复 ECL 信号用于检测 ACE,检测限为 4.74 ×10-15 M。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Analytica Chimica Acta
Analytica Chimica Acta 化学-分析化学
CiteScore
10.40
自引率
6.50%
发文量
1081
审稿时长
38 days
期刊介绍: Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.
期刊最新文献
Automatic and Integrated Detection of Nucleic Acid by Using a Dual-mode Thermal Controlled Digital Microfluidic Chip Dual emission chiral carbon dots as fluorescent probe for fast chiral recognition of tryptophan enantiomers Ratiometric fluorescent paper chip for monitoring the freshness of high protein foods Enhanced Stability of Hairpin-Functionalized DNA Tetrahedral Nanostructures for miRNA Detection in Plasma from Ischemic Stroke Patients Conjugated Polymer-Boosted Near-infrared Electrochemiluminescence of Organic Dye for Detecting Acetamiprid
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1