Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals

IF 51.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Chemical Reviews Pub Date : 2024-11-13 DOI:10.1021/acs.chemrev.4c00293
Anneliese M. M. Gest, Ayse Z. Sahan, Yanghao Zhong, Wei Lin, Sohum Mehta, Jin Zhang
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Abstract

Cellular function is controlled through intricate networks of signals, which lead to the myriad pathways governing cell fate. Fluorescent biosensors have enabled the study of these signaling pathways in living systems across temporal and spatial scales. Over the years there has been an explosion in the number of fluorescent biosensors, as they have become available for numerous targets, utilized across spectral space, and suited for various imaging techniques. To guide users through this extensive biosensor landscape, we discuss critical aspects of fluorescent proteins for consideration in biosensor development, smart tagging strategies, and the historical and recent biosensors of various types, grouped by target, and with a focus on the design and recent applications of these sensors in living systems.

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行动中的分子间谍基因编码荧光生物传感器点亮细胞信号
细胞功能是通过错综复杂的信号网络控制的,这些信号网络导致了无数影响细胞命运的途径。荧光生物传感器使人们能够跨越时间和空间尺度研究生命系统中的这些信号通路。多年来,荧光生物传感器的数量激增,因为它们可用于众多目标,跨越光谱空间,适合各种成像技术。为了引导用户了解这一广泛的生物传感器领域,我们将讨论在生物传感器开发过程中需要考虑的荧光蛋白的关键方面、智能标记策略,以及历史和最新的各类生物传感器(按目标分组),并重点介绍这些传感器在生命系统中的设计和最新应用。
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来源期刊
Chemical Reviews
Chemical Reviews 化学-化学综合
CiteScore
106.00
自引率
1.10%
发文量
278
审稿时长
4.3 months
期刊介绍: Chemical Reviews is a highly regarded and highest-ranked journal covering the general topic of chemistry. Its mission is to provide comprehensive, authoritative, critical, and readable reviews of important recent research in organic, inorganic, physical, analytical, theoretical, and biological chemistry. Since 1985, Chemical Reviews has also published periodic thematic issues that focus on a single theme or direction of emerging research.
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