Metabolic syndrome promotes resistance to aspirin in mitigating bone loss in murine periodontal disease.

IF 5.2 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL Life sciences Pub Date : 2024-11-06 DOI:10.1016/j.lfs.2024.123224

Lucas Sobral de Rossi, Raquel Pires Nakama, Lucas Felipe Dos Santos, Leonardo Berto-Pereira, Aparecida Donizette Malvezi, Maria Isabel Lovo-Martins, Ana Paula Canizares Cardoso, Luiz Claúdio Tozoni-Filho, Eduardo Inocente Jussiani, Andressa Mendes Dionísio de Freitas, Marli Cardoso Martins-Pinge, Phileno Pinge-Filho

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Abstract

Aims: This study aimed to investigate the protective effects of aspirin (ASA) on alveolar bone loss in a mouse model with metabolic syndrome (MetS) and concurrent periodontal disease (PD). Specifically, the study sought to determine whether ASA could mitigate bone loss in MetS and non-MetS animals with PD and explore the correlation between gingival nitric oxide (NO) levels and bone resorption.

Main methods: Newborn female Swiss mice were administered monosodium glutamate (MSG) (4 mg/g) during the initial 5 days of life to induce MetS (MetS group), while the control group (SAL) was administered saline. On the 60th day, PD was induced in both groups. Half of the animals were treated daily with ASA (40 mg/kg). MetS was characterized by the Lee index, blood glucose, and cardiovascular parameters. Maxillae were evaluated by microtomography and histopathology, showing significant bone loss after PD induction.

Key findings: Animals with MetS exhibited higher alveolar bone loss than controls. SAL animals treated with ASA had less bone loss than their MetS counterparts. Gingival NO levels were elevated in animals with PD, and a strong correlation was found between NO levels and bone resorption. ASA reduced NO in non-MetS animals, but MetS animals were resistant to this effect.

Significance: These findings suggest a protective mechanism of ASA against bone loss in non-MetS animals with PD, an effect that was not observed in MetS animals. Consequently, this study provides novel insights into the intricate relationship between MetS and PD in mice.

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代谢综合征会导致阿司匹林在减轻小鼠牙周病骨质流失方面产生抗药性。

目的：本研究旨在探讨阿司匹林（ASA）对代谢综合征（MetS）和并发牙周病（PD）小鼠模型牙槽骨流失的保护作用。具体来说，该研究试图确定阿司匹林是否能减轻代谢综合征和非代谢综合征动物的牙周病骨质流失，并探讨牙龈一氧化氮（NO）水平与骨吸收之间的相关性：主要方法：新生雌性瑞士小鼠在出生后最初5天内服用谷氨酸钠（MSG）（4毫克/克）诱导MetS（MetS组），而对照组（SAL）服用生理盐水。在第60天，两组动物均诱发帕金森病。半数动物每天服用ASA（40毫克/千克）。通过李氏指数、血糖和心血管参数对 MetS 进行表征。通过显微断层扫描和组织病理学对上颌骨进行评估，结果显示诱导PD后骨质明显流失：主要发现：与对照组相比，患有 MetS 的动物表现出更高的牙槽骨损失。与 MetS 动物相比，接受 ASA 治疗的 SAL 动物骨质流失较少。PD动物的牙龈NO水平升高，NO水平与骨吸收之间存在很强的相关性。ASA 可减少非 MetS 动物体内的 NO，但 MetS 动物对这种效应具有抵抗力：这些研究结果表明，在患有帕金森病的非 MetS 动物中，ASA 对骨质流失具有保护作用，而在 MetS 动物中却未观察到这种作用。因此，本研究为了解小鼠 MetS 与帕金森病之间错综复杂的关系提供了新的视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Life sciences 医学-药学

CiteScore

12.20

自引率

1.60%

发文量

841

审稿时长

6 months

期刊介绍： Life Sciences is an international journal publishing articles that emphasize the molecular, cellular, and functional basis of therapy. The journal emphasizes the understanding of mechanism that is relevant to all aspects of human disease and translation to patients. All articles are rigorously reviewed. The Journal favors publication of full-length papers where modern scientific technologies are used to explain molecular, cellular and physiological mechanisms. Articles that merely report observations are rarely accepted. Recommendations from the Declaration of Helsinki or NIH guidelines for care and use of laboratory animals must be adhered to. Articles should be written at a level accessible to readers who are non-specialists in the topic of the article themselves, but who are interested in the research. The Journal welcomes reviews on topics of wide interest to investigators in the life sciences. We particularly encourage submission of brief, focused reviews containing high-quality artwork and require the use of mechanistic summary diagrams.