A novel sensing probe based on AuNPs-Apt for the detection of enrofloxacin.

IF 2.9 4区材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY Nanotechnology Pub Date : 2024-11-08 DOI:10.1088/1361-6528/ad8c4b

Liyun Ding, Pan Wang, BaoQuan Xiao, Zelin Gao, Dekui Zhang, Tuanjie Che, Juanjuan Zuo, Yumai Li

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Abstract

Enrofloxacin (ENR), as a synthetic broad-spectrum antibiotic is widely utilized in veterinary medicine to treat animal diseases and promote livestock growth, it can inhibit bacterial DNA gyrase subunit A, thereby preventing bacterial DNA replication and exerting its antibacterial effect. However, excessive use of enrofloxacin poses significant risks to ecological balance and human health due to residual contamination. We have developed a novel ENR aptamer sensor based on the gold nanoparticles/aptamer (AuNPs-Apt) complexes, in which AuNPs were synthesized via the seed method and functionalized with aptamers. The optical properties, particle size, functional groups and morphology of the AuNPs-Apt probe were characterized by transmission electron microscope, Fourier transform infrared spectrometer and UV-vis spectrophotometer, respectively. The aptamer biosensor can specifically identify enrofloxacin, with a wide detection range (0.05-100μg ml^-1) and a good linear relationship (R2=0.99) within the detection range. In addition, the biosensor also has the advantages of short detection time, low biological toxicity, good stability, and low detection cost. Therefore, it shows a great prospect for practical application in the field of detecting enrofloxacin residues.

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基于 AuNPs-Apt 的新型传感探针用于检测恩诺沙星。

恩诺沙星（ENR）作为一种人工合成的广谱抗生素，被广泛应用于兽医领域，用于治疗动物疾病和促进牲畜生长，它能抑制细菌 DNA 回旋酶亚基 A，从而阻止细菌 DNA 复制，发挥抗菌作用。然而，过量使用恩诺沙星会造成残留污染，给生态平衡和人类健康带来巨大风险。我们开发了一种基于金纳米颗粒/适配体（AuNPs-Apt）复合物的新型恩诺沙星适配体传感器。透射电子显微镜、傅立叶变换红外光谱仪和紫外-可见分光光度计分别对 AuNPs-Apt 探针的光学性质、粒度、功能基团和形态进行了表征。该aptamer 生物传感器可特异性地识别恩诺沙星，检测范围广（0.05-100μg ml-1），且在检测范围内具有良好的线性关系（R2=0.99）。此外，该生物传感器还具有检测时间短、生物毒性低、稳定性好、检测成本低等优点。因此，它在恩诺沙星残留检测领域具有广阔的实际应用前景。

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来源期刊

Nanotechnology 工程技术-材料科学：综合

CiteScore

7.10

自引率

5.70%

发文量

820

审稿时长

2.5 months

期刊介绍： The journal aims to publish papers at the forefront of nanoscale science and technology and especially those of an interdisciplinary nature. Here, nanotechnology is taken to include the ability to individually address, control, and modify structures, materials and devices with nanometre precision, and the synthesis of such structures into systems of micro- and macroscopic dimensions such as MEMS based devices. It encompasses the understanding of the fundamental physics, chemistry, biology and technology of nanometre-scale objects and how such objects can be used in the areas of computation, sensors, nanostructured materials and nano-biotechnology.