BOLA family genes are the drivers and potential biomarkers of survival in kidney renal clear cell carcinoma patients.

IF 1.7 4区医学 Q2 MEDICINE, GENERAL & INTERNAL Saudi Medical Journal Pub Date : 2024-11-01 DOI:10.15537/smj.2024.45.11.20240604

Mohammed Alissa, Abdullah Alghamdi, Suad A Alghamdi, Mohammed A Alshehri, Meshari A Alsuwat, Mamdouh Allahyani, Ali G Alkhathami

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Abstract

Objectives: To analyze the diagnostic and prognostic potential of the BOLA gene family in kidney renal clear cell carcinoma (KIRC).

Methods: This study is an observational study. The whole study was carried out at Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia from January to November 2023. As it involves in silico and in vitro methods, ethical consent was not required. Various in silico and molecular experimental techniques were employed in this study.

Results: Significant up-regulation and hypomethylation of BOLA1, BOLA2, and BOLA3 were observed across 12 KIRC cell lines. Retrospective analysis of the cancer genome atlas program (TCGA)-KIRC cohorts confirmed hypomethylation of these genes in KIRC tissues. The cBioPortal analysis showed minimal genetic alterations, with amplification being the most common. Kaplan-Meier plotter data revealed that high BOLA1, BOLA2, and BOLA3 expression correlated with shorter overall survival and relapse-free survival in KIRC. Tumor-immune system interactions database analysis linked BOLA1 expression to immune and molecular subtypes. Gene silencing of BOLA1, BOLA2, and BOLA3 in 786-0 cells reduced cell growth and proliferation, enhancing wound healing capacity.

Conclusion: BOLA genes may serve as diagnostic and prognostic markers in KIRC, offering insights into therapeutic targets and disease progression.

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BOLA家族基因是肾透明细胞癌患者生存的驱动因素和潜在生物标志物。

目的：分析BOLA基因家族在肾透明细胞癌（KIRC）中的诊断和预后潜力：分析 BOLA 基因家族在肾透明细胞癌（KIRC）中的诊断和预后潜力：本研究为观察性研究。整个研究于 2023 年 1 月至 11 月在沙特阿拉伯 Al-Kharj 的萨塔姆-本-阿卜杜勒阿齐兹王子大学（Prince Sattam bin Abdulaziz University）进行。由于研究涉及硅学和体外方法，因此无需征得伦理同意。本研究采用了多种硅学和分子实验技术：结果：在 12 个 KIRC 细胞系中观察到 BOLA1、BOLA2 和 BOLA3 的显著上调和低甲基化。对癌症基因组图谱计划（TCGA）-KIRC 队列的回顾性分析证实了这些基因在 KIRC 组织中的低甲基化。cBioPortal 分析显示基因改变极少，扩增最为常见。Kaplan-Meier plotter数据显示，BOLA1、BOLA2和BOLA3的高表达与KIRC较短的总生存期和无复发生存期相关。肿瘤-免疫系统相互作用数据库分析将 BOLA1 的表达与免疫和分子亚型联系起来。786-0细胞中BOLA1、BOLA2和BOLA3的基因沉默可减少细胞生长和增殖，提高伤口愈合能力：结论：BOLA 基因可作为 KIRC 的诊断和预后标志物，为治疗目标和疾病进展提供洞察力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Saudi Medical Journal 医学-医学：内科

CiteScore

2.30

自引率

6.20%

发文量

203

审稿时长

12 months

期刊介绍： The Saudi Medical Journal is a monthly peer-reviewed medical journal. It is an open access journal, with content released under a Creative Commons attribution-noncommercial license. The journal publishes original research articles, review articles, Systematic Reviews, Case Reports, Brief Communication, Brief Report, Clinical Note, Clinical Image, Editorials, Book Reviews, Correspondence, and Student Corner.