DRN facilitates WUS transcriptional regulatory activity by chromatin remodeling to regulate shoot stem cell homeostasis in Arabidopsis.

IF 9.8 1区生物学 Q1 Agricultural and Biological Sciences PLoS Biology Pub Date : 2024-11-08 eCollection Date: 2024-11-01 DOI:10.1371/journal.pbio.3002878

Linjie Luo, Li Liu, Lili She, Haoran Zhang, Nannan Zhang, Yaqin Wang, Yuting Ni, Fugui Chen, Fengying Wan, Yuqiu Dai, Guoping Zhu, Zhong Zhao

{"title":"DRN facilitates WUS transcriptional regulatory activity by chromatin remodeling to regulate shoot stem cell homeostasis in Arabidopsis.","authors":"Linjie Luo, Li Liu, Lili She, Haoran Zhang, Nannan Zhang, Yaqin Wang, Yuting Ni, Fugui Chen, Fengying Wan, Yuqiu Dai, Guoping Zhu, Zhong Zhao","doi":"10.1371/journal.pbio.3002878","DOIUrl":null,"url":null,"abstract":"<p><p>Shoot stem cells, harbored in the shoot apical meristem (SAM), play key roles during post-embryonic development of Arabidopsis and function as the origin of plant aerial tissues. Multiple transcription factors are involved in the sophisticated transcriptional regulation of stem cell homeostasis, with the WUSCHEL (WUS)/CLAVATA3 (CLV3) negative feedback loop playing a central role. WUS acts as a master regulator in maintaining stem cells through its transcriptional regulatory activity including repressive and activating abilities. Although the interaction between WUS and TOPLESS confers the repressive activity of WUS in transcriptional control, the mechanism by which WUS activates gene expression remains elusive. Here, we showed that DORNRÖSCHEN competitively interacts with WUS and disturbs the WUS homodimer, which recruits BRAHMA to activate CLV3 expression via nucleosome depletion for maintaining the stem cell pool.</p>","PeriodicalId":49001,"journal":{"name":"PLoS Biology","volume":null,"pages":null},"PeriodicalIF":9.8000,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"PLoS Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1371/journal.pbio.3002878","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}

引用次数: 0

Abstract

Shoot stem cells, harbored in the shoot apical meristem (SAM), play key roles during post-embryonic development of Arabidopsis and function as the origin of plant aerial tissues. Multiple transcription factors are involved in the sophisticated transcriptional regulation of stem cell homeostasis, with the WUSCHEL (WUS)/CLAVATA3 (CLV3) negative feedback loop playing a central role. WUS acts as a master regulator in maintaining stem cells through its transcriptional regulatory activity including repressive and activating abilities. Although the interaction between WUS and TOPLESS confers the repressive activity of WUS in transcriptional control, the mechanism by which WUS activates gene expression remains elusive. Here, we showed that DORNRÖSCHEN competitively interacts with WUS and disturbs the WUS homodimer, which recruits BRAHMA to activate CLV3 expression via nucleosome depletion for maintaining the stem cell pool.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

DRN通过染色质重塑促进WUS转录调控活动，从而调节拟南芥芽干细胞的稳态。

生长在嫩枝顶端分生组织（SAM）中的嫩枝干细胞在拟南芥胚后发育过程中发挥着关键作用，是植物气生组织的起源。多种转录因子参与了干细胞平衡的复杂转录调控，其中 WUSCHEL（WUS）/CLAVATA3（CLV3）负反馈环发挥了核心作用。WUS通过其转录调控活性，包括抑制和激活能力，在维持干细胞方面发挥着主调控因子的作用。虽然WUS和TOPLESS之间的相互作用赋予了WUS在转录调控中的抑制活性，但WUS激活基因表达的机制仍未确定。在这里，我们发现DORNRÖSCHEN与WUS竞争性相互作用，干扰了WUS同源二聚体，后者招募BRAHMA通过核小体耗竭激活CLV3表达，以维持干细胞池。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

PLoS Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-BIOLOGY

CiteScore

15.40

自引率

2.00%

发文量

359

审稿时长

3-8 weeks

期刊介绍： PLOS Biology is the flagship journal of the Public Library of Science (PLOS) and focuses on publishing groundbreaking and relevant research in all areas of biological science. The journal features works at various scales, ranging from molecules to ecosystems, and also encourages interdisciplinary studies. PLOS Biology publishes articles that demonstrate exceptional significance, originality, and relevance, with a high standard of scientific rigor in methodology, reporting, and conclusions. The journal aims to advance science and serve the research community by transforming research communication to align with the research process. It offers evolving article types and policies that empower authors to share the complete story behind their scientific findings with a diverse global audience of researchers, educators, policymakers, patient advocacy groups, and the general public. PLOS Biology, along with other PLOS journals, is widely indexed by major services such as Crossref, Dimensions, DOAJ, Google Scholar, PubMed, PubMed Central, Scopus, and Web of Science. Additionally, PLOS Biology is indexed by various other services including AGRICOLA, Biological Abstracts, BIOSYS Previews, CABI CAB Abstracts, CABI Global Health, CAPES, CAS, CNKI, Embase, Journal Guide, MEDLINE, and Zoological Record, ensuring that the research content is easily accessible and discoverable by a wide range of audiences.