Enhanced Mandibular Bone Repair Using Poly Lactic-co-glycolic Acid Combined with Nanohydroxyapatite Scaffold Loaded by Mesenchymal Stromal/Stem Cells and Curcumin in Male Rats

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Biomaterials Science & Engineering Pub Date : 2024-10-28 DOI:10.1021/acsbiomaterials.4c0060810.1021/acsbiomaterials.4c00608

Mohamad Mokhtarzadegan, Saba Amini, Aida Iraji, Mehdi Kian, Cambyz Irajie, Seyyed Sajad Daneshi, Shekofeh Abbaspour, Shahrokh Zare, Akram Jamshidzadeh, Ali Feiz, Nadiar M. Mussin, Nader Tanideh* and Amin Tamadon*,

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Abstract

This study aimed to investigate the healing effect of a polylactic-co-glycolic acid (PLGA) scaffold containing nanohydroxyapatite (NHA) along with curcumin (CCM), loaded with adipose-derived mesenchymal stem cells (AD-MSCs), on mandibular bone defects. The designed PLGA scaffolds containing NHA were evaluated for their mechanical and structural properties. Forty rats were divided into five groups (n = 8) based on the treatment: Sham, PLGA scaffolds containing NHA, PLGA scaffolds containing NHA + CCM, PLGA scaffolds containing NHA + AD-MSCs, and PLGA scaffolds containing NHA + CCM + AD-MSCs. After 8 weeks’ follow-up, mandible bones were isolated for histomorphometry evaluation. Data were analyzed using SPSS version 21, with p-values <0.05 considered statistically significant. SEM evaluation showed that the designed nanocomposite scaffold had 80% porosity. Histomorphometry results indicated a significant difference in osteocyte, osteoblast, bone area, and vascular area parameters in the group treated with scaffolds loaded with AD-MSCs + CCM compared to the other groups (p < 0.05). The PLGA-containing NHA-CCM nanocomposite scaffold demonstrated good porosity and dispersion, suitable for treating bone defects. Rats treated with scaffolds containing AD-MSCs and CCM showed better therapeutic results than the other groups. Further research is needed to evaluate its anti-inflammatory, antioxidant properties, osteogenesis, and therapeutic effects in larger animal models.

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在雄性大鼠体内使用含有间充质基质/干细胞和姜黄素的聚乳酸-共-乙醇酸与纳米羟基磷灰石支架增强下颌骨修复效果

本研究旨在探讨一种含有纳米羟基磷灰石（NHA）和姜黄素（CCM）的聚乳酸-聚羟基乙酸（PLGA）支架负载脂肪间充质干细胞（AD-MSCs）对下颌骨缺损的愈合效果。对所设计的含有 NHA 的 PLGA 支架的机械和结构特性进行了评估。40 只大鼠按处理方法分为 5 组（n = 8）：假体组、含 NHA 的 PLGA 支架组、含 NHA + CCM 的 PLGA 支架组、含 NHA + AD-MSCs 的 PLGA 支架组和含 NHA + CCM + AD-MSCs 的 PLGA 支架组。随访 8 周后，分离下颌骨进行组织形态学评估。数据采用 SPSS 21 版进行分析，P 值为 0.05，具有统计学意义。扫描电镜评估显示，设计的纳米复合材料支架具有 80% 的孔隙率。组织形态学结果表明，与其他组相比，用 AD-MSCs + CCM 载荷的支架处理组的骨细胞、成骨细胞、骨面积和血管面积参数有显著差异（p <0.05）。含 PLGA 的 NHA-CCM 纳米复合材料支架具有良好的孔隙率和分散性，适用于治疗骨缺损。用含有 AD-MSCs 和 CCM 的支架治疗大鼠显示出比其他组更好的治疗效果。还需要进一步研究，以评估其在更大动物模型中的抗炎、抗氧化特性、成骨和治疗效果。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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