Deciphering the dead zone on anammox system in biofilters

Abstract

In an anammox biofilm reactor, long-term operation inevitably leads to the repeated formation of localized dead zones. Once these dead zones (DZs) occur, the anammox reactor’s nitrogen removal efficiency is severely reduced. However, the mechanisms and intrinsic reasons for the transformation of DZs remain unexplored. In this study, the pilot-scale biofilters were classified into biologically active zones (BZs), transition zones (TZs), and DZs. The results indicated that microbial communities undergo accelerated succession from the TZ. Biofilms respond to environmental stress from the DZs by altering the levels of signaling molecules, triggering a series of cascading reactions. These reactions alter the abundance of genes involved in nitrogen removal, promote substance transformation, and speed up the succession of microbial communities. This study demonstrates the objectives and self-healing mechanisms of the anammox biofilm process in the presence of dead zones, which could support the long-term application of anammox technology.