Glycyrrhizin alleviated cisplatin-induced testicular injury by inhibiting the oxidative, apoptotic, hormonal, and histological alterations.

IF 1.3 3区农林科学 Q2 VETERINARY SCIENCES American journal of veterinary research Pub Date : 2024-11-07 DOI:10.2460/ajvr.24.10.0288

Fawiziah Khalaf Alharbi, Lashin Saad Ali, Gamal A Salem, Naira ElAshmouny, Sanad S El-Kholy, Walaa M Essawi, Azza I Helal, Hany Sabry A Ibrahim, Naief Dahran, Eman S El-Shetry, Rania Hassan Mohamed Soliman, Hassan Emam, Mamdouh Eldesoqui, Fahmy Gad Elsaid, Fawziah A Al-Salmi, Doaa Abdelrahaman, Eman Fayad, Abdel-Rahman A Sobeih, Wael A M Ghonimi

{"title":"Glycyrrhizin alleviated cisplatin-induced testicular injury by inhibiting the oxidative, apoptotic, hormonal, and histological alterations.","authors":"Fawiziah Khalaf Alharbi, Lashin Saad Ali, Gamal A Salem, Naira ElAshmouny, Sanad S El-Kholy, Walaa M Essawi, Azza I Helal, Hany Sabry A Ibrahim, Naief Dahran, Eman S El-Shetry, Rania Hassan Mohamed Soliman, Hassan Emam, Mamdouh Eldesoqui, Fahmy Gad Elsaid, Fawziah A Al-Salmi, Doaa Abdelrahaman, Eman Fayad, Abdel-Rahman A Sobeih, Wael A M Ghonimi","doi":"10.2460/ajvr.24.10.0288","DOIUrl":null,"url":null,"abstract":"Objective: To evaluate the potential contribution of glycyrrhizin (GLZ) to mitigate the testicular toxicity linked to cisplatin (CIS) intoxication.Methods: 40 mature male Wistar albino rats (Rattus norvegicus albinus) were randomly divided into 4 equal groups (n = 10) for 60 days: the control group, CIS-treated group (single dose of 7 mg/kg, IP), GLZ-treated group (25 mg/kg, PO), and GLZ plus CIS-treated group. Blood and testis samples were examined using biochemical, histological, and immunohistochemical techniques. Semen samples were also obtained, and any abnormalities were reported.Results: Serum follicle-stimulating hormone, luteinizing hormone, and testosterone levels were all markedly reduced by CIS. Oxidative stress and a significant reduction in levels of the antioxidant enzymes glutathione peroxidase, superoxide dismutase, and catalase were linked to CIS. Immunohistochemically, CIS showed diffuse, significantly positive immunolocalizations against the anti-caspase 3 antibody, indicating widespread apoptosis within the testicular parenchyma. Histopathologically, CIS showed diffuse coagulative necrosis of spermatogenic cells, necrotic Sertoli cells, intertubular edema, and Leydig cell hyperplasia. Moreover, CIS revealed a noteworthy increase in sperm abnormalities. Pre-coadministration and posttreatment with GLZ mitigated the majority of these detrimental consequences, and serum levels of antioxidant enzymes, luteinizing hormone, follicle-stimulating hormone, and testosterone were significantly elevated.Conclusions: Glycyrrhizin has been proven to be a strong antioxidant as well as antiapoptotic and cytoprotective against CIS testicular damage.Clinical relevance: The described model is a tool to evaluate the testicular protective impact of GLZ.","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-13"},"PeriodicalIF":1.3000,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2460/ajvr.24.10.0288","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: To evaluate the potential contribution of glycyrrhizin (GLZ) to mitigate the testicular toxicity linked to cisplatin (CIS) intoxication.

Methods: 40 mature male Wistar albino rats (Rattus norvegicus albinus) were randomly divided into 4 equal groups (n = 10) for 60 days: the control group, CIS-treated group (single dose of 7 mg/kg, IP), GLZ-treated group (25 mg/kg, PO), and GLZ plus CIS-treated group. Blood and testis samples were examined using biochemical, histological, and immunohistochemical techniques. Semen samples were also obtained, and any abnormalities were reported.

Results: Serum follicle-stimulating hormone, luteinizing hormone, and testosterone levels were all markedly reduced by CIS. Oxidative stress and a significant reduction in levels of the antioxidant enzymes glutathione peroxidase, superoxide dismutase, and catalase were linked to CIS. Immunohistochemically, CIS showed diffuse, significantly positive immunolocalizations against the anti-caspase 3 antibody, indicating widespread apoptosis within the testicular parenchyma. Histopathologically, CIS showed diffuse coagulative necrosis of spermatogenic cells, necrotic Sertoli cells, intertubular edema, and Leydig cell hyperplasia. Moreover, CIS revealed a noteworthy increase in sperm abnormalities. Pre-coadministration and posttreatment with GLZ mitigated the majority of these detrimental consequences, and serum levels of antioxidant enzymes, luteinizing hormone, follicle-stimulating hormone, and testosterone were significantly elevated.

Conclusions: Glycyrrhizin has been proven to be a strong antioxidant as well as antiapoptotic and cytoprotective against CIS testicular damage.

Clinical relevance: The described model is a tool to evaluate the testicular protective impact of GLZ.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

甘草酸苷通过抑制氧化、凋亡、激素和组织学改变，减轻了顺铂诱导的睾丸损伤。

目的：评估甘草酸苷（GLZ）对减轻顺铂中毒引起的睾丸毒性的潜在作用：方法：将40只成熟雄性Wistar白化大鼠（Rattus norvegicus albinus）随机分为4组（n = 10），每组60天：对照组、CIS处理组（单剂量7 mg/kg，IP）、GLZ处理组（25 mg/kg，PO）和GLZ加CIS处理组。采用生化、组织学和免疫组化技术检查血液和睾丸样本。此外，还采集了精液样本，并报告了任何异常情况：结果：CIS 使血清卵泡刺激素、黄体生成素和睾酮水平明显下降。氧化应激和抗氧化酶谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶水平的显著降低与 CIS 有关。免疫组织化学方面，CIS 显示出抗天冬酶 3 抗体的弥漫性、显著阳性的免疫定位，表明睾丸实质内存在广泛的细胞凋亡。组织病理学方面，CIS显示生精细胞弥漫性凝固性坏死、Sertoli细胞坏死、管间水肿和Leydig细胞增生。此外，CIS 还显示精子畸形明显增加。甘草酸苷的给药前和给药后处理减轻了这些有害后果中的大部分，血清中的抗氧化酶、促黄体生成素、促卵泡激素和睾酮水平显著升高：结论：事实证明，甘草酸苷是一种强抗氧化剂，还具有抗凋亡和细胞保护作用，可防止 CIS 对睾丸的损伤：所述模型是评估 GLZ 对睾丸保护作用的一种工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

American journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

10.00%

发文量

186

审稿时长

3 months

期刊介绍： The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.