A Chromosome-Level Genome Assembly of Chiton Acanthochiton rubrolineatus (Chitonida, Polyplacophora, Mollusca).

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Animals Pub Date : 2024-11-04 DOI:10.3390/ani14213161

Jiangyong Qu, Xiaofei Lu, Chenen Tu, Fuyang He, Sutao Li, Dongyue Gu, Shuang Wang, Zhikai Xing, Li Zheng, Xumin Wang, Lijun Wang

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Abstract

(1) Background: Chitons (Mollusca, Polyplacophora) are relatively primitive species in Mollusca that allow the study of biomineralization. Although mitochondrial genomes have been isolated from Polyplacophora, there is no genomic information at the chromosomal level; (2) Methods: Here we report a chromosome-level genome assembly for Acanthochiton rubrolineatus using PacBio (Pacific Biosciences, United States) reads and high-throughput chromosome conformation capture (Hi-C) data; (3) Results: The assembly spans 1.08 Gb with a contig N50 of 3.63 Mb and 99.97% of the genome assigned to eight chromosomes. Among the 32,291 predicted genes, 76.32% had functional predictions. The divergence time of Brachiopoda and Mollusca was ~550.8 Mya (million years ago), and that of A. rubrolineatus and other mollusks was ~548.5 Mya; (4) Conclusions: This study not only offers high-quality reference sequences for the Acanthochiton rubrolineatus genome, but also establishes groundwork for investigating the mechanisms of Polyplacophora biomineralization and its evolutionary history. This research will aid in uncovering the genetic foundations of molluscan adaptations across diverse environments.

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甲壳动物 Acanthochiton rubrolineatus（甲壳纲，多孔目，软体动物）染色体级基因组组装。

(1) 背景：甲壳动物（软体动物门，多孔动物）是软体动物门中相对原始的物种，可用于研究生物矿化。虽然线粒体基因组已从多孔动物中分离出来，但染色体水平上的基因组信息还没有；（2）方法：在此，我们利用 PacBio（美国太平洋生物科学公司）的读数和高通量染色体构象捕获（Hi-C）数据，报告了 Acanthochiton rubrolineatus 的染色体水平基因组组装情况；（3）结果：基因组组装的跨度为 1.08 Gb，等位基因 N50 为 3.63 Mb，99.97% 的基因组被分配到 8 条染色体上。在 32 291 个预测基因中，76.32%具有功能预测。腕足动物和软体动物的分化时间约为 550.8 Mya（百万年前），A. rubrolineatus 和其他软体动物的分化时间约为 548.5 Mya；（4）结论：这项研究不仅为Acanthochiton rubrolineatus基因组提供了高质量的参考序列，而且为研究多孔动物的生物矿化机制及其进化历史奠定了基础。这项研究将有助于揭示软体动物适应不同环境的遗传基础。

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来源期刊

Animals Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

4.90

自引率

16.70%

发文量

3015

审稿时长

20.52 days

期刊介绍： Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).