Red light-emitting diode on skin healing: an in vitro and in vivo experimental study.

IF 2.6 4区医学 Q2 DERMATOLOGY Anais brasileiros de dermatologia Pub Date : 2024-11-08 DOI:10.1016/j.abd.2024.02.008

Tuany R Schmidt, Belkiss C Mármora, Fernanda T Brochado, Lucas Gonçalves, Paloma S Campos, Marcelo L Lamers, Aurigena A de Araújo, Caroline A C X de Medeiros, Susana B Ribeiro, Marco A T Martins, Emily F S Pilar, Manoela D Martins, Vivian P Wagner

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Abstract

Background: The clinical advantages of light-emitting diode (LED) therapy in skin healing and its underlying mechanism remain subjects of ongoing debate.

Objective: This study aims to explore the impact of LED therapy on normal skin keratinocytes (HaCaT) and in the repair of full-thickness dorsal wounds in Wistar rats.

Methods: HaCaT cell viability (SRB assay) and migration (scratch assay) were assessed under LED therapy, comparing stress conditions (2.5% FBS) with sham irradiation and optimal conditions (10% FBS). In vivo, 50 rats with induced wounds were divided into Sham and LED (daily treatment) groups. Euthanasia occurred at 3, 5, 10, 14, and 21 days for clinical, morphological, oxidative stress (MDA, SOD, and GSH), and cytokine analyses (IL-1β, IL-10, TNF-α).

Results: LED therapy significantly enhanced keratinocytes viability compared to sham irradiation, with minimal impact on cell migration. Clinical benefits were prominent on day 10, influencing inflammation progression and resolution on days 3 and 10. Re-epithelization remained unaffected. Reduced MDA and increased GSH levels were observed throughout, while SOD levels varied temporally. Notably, on day 10, LED significantly decreased IL-1β, IL-10, and TNF-α.

Study limitations: Although translational, clinical trial confirmation of observed benefits is warranted.

Conclusions: LED therapy expedites cutaneous healing in the experimental model, primarily modulating inflammation and enhancing antioxidant activity.

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红色发光二极管对皮肤愈合的影响：体外和体内实验研究。

背景：发光二极管（LED）疗法在皮肤愈合方面的临床优势及其内在机制仍是争论不休的话题：方法：将应激条件（2.5% FBS）与假照射和最佳条件（10% FBS）进行比较，评估 LED 治疗下 HaCaT 细胞的活力（SRB 试验）和迁移（划痕试验）。在体内，50 只诱导伤口的大鼠被分为 Sham 组和 LED（每日治疗）组。安乐死大鼠3、5、10、14和21天后进行临床、形态学、氧化应激（MDA、SOD和GSH）和细胞因子（IL-1β、IL-10、TNF-α）分析：结果：与假照射相比，LED疗法明显提高了角朊细胞的活力，但对细胞迁移的影响很小。临床疗效在第 10 天显著显现，影响了炎症的进展和第 3 天和第 10 天的消退。重新上皮仍然不受影响。在整个过程中，MDA 水平降低，GSH 水平升高，而 SOD 水平则随时间变化。值得注意的是，在第 10 天，发光二极管显著降低了 IL-1β、IL-10 和 TNF-α：研究局限性：虽然是转化性研究，但观察到的益处还需要临床试验的证实：结论：在实验模型中，LED疗法可加速皮肤愈合，主要是调节炎症和增强抗氧化活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anais brasileiros de dermatologia DERMATOLOGY-

CiteScore

2.40

自引率

0.00%

发文量

184

审稿时长

32 days

期刊介绍： The journal is published bimonthly and is devoted to the dissemination of original, unpublished technical-scientific study, resulting from research or reviews of dermatological topics and related matters. Exchanges with other publications may be accepted.