Triple-template surface imprinted magnetic polymers for wide-coverage extraction of steroid hormones from human serum.

IF 1.8 4区化学 Q3 CHEMISTRY, ANALYTICAL Analytical Sciences Pub Date : 2024-11-11 DOI:10.1007/s44211-024-00690-9

Li Wang, Hongyu Wu, Caihong Wang, Yunfei Ma, Zheng Xiang

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Abstract

In this study, novel triple-template magnetic nanospheres by surface imprinting, called triple-template magnetic molecularly imprinted polymers (tri-MMIPs), were prepared for the detection of steroid hormones in human serum. The polymers were constructed by Fe₃O₄ as support, estrone (E1), progesterone (PROG), and estradiol (E2) as triple templates, acrylic acid (AA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The resulting tri-MMIPs were further characterized and applied as sorbents in human serum pretreatment. Coupled with ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry (UHPLC-MS/MS) profiling after derivatization, 15 steroids (E1, 6-ketoE1, 4-OHE1, 16α-OHE1, DHEA, 4-MeOE1, PROG, PREG, 17-OHPROG, 7-DHE2, E2, 2-OHE2, 16-epiE3, E3, 2-MeOE2) were successfully quantified. The developed method exhibited a good recovery (78.9-114%) and low limit of quantitation (LLOQ, 5-10 pg/mL), which indicates a great potential for application in the analysis of multiple steroid hormones in complex biological samples.

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用于从人体血清中大范围提取类固醇激素的三模板表面印迹磁性聚合物。

本研究通过表面印迹法制备了新型三模板磁性纳米球，称为三模板磁性分子印迹聚合物（tri-MMIPs），用于检测人体血清中的类固醇激素。这种聚合物以 Fe3O4 为载体，雌酮（E1）、孕酮（PROG）和雌二醇（E2）为三重模板，丙烯酸（AA）为功能单体，乙二醇二甲基丙烯酸酯（EGDMA）为交联剂。所得的三MMIPs得到了进一步表征，并被用作人血清预处理的吸附剂。衍生化后，结合超高效液相色谱-三重四极杆串联质谱（UHPLC-MS/MS）分析，成功定量了 15 种类固醇（E1、6-酮E1、4-OHE1、16α-OHE1、DHEA、4-MeOE1、PROG、PREG、17-OHPROG、7-DHE2、E2、2-OHE2、16-epiE3、E3、2-MeOE2）。该方法回收率高（78.9-114%），定量限低（5-10 pg/mL），在复杂生物样品中多种甾体激素的分析中具有很大的应用潜力。

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来源期刊

Analytical Sciences 化学-分析化学

CiteScore

2.90

自引率

18.80%

发文量

232

审稿时长

1 months

期刊介绍： Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.