Dual-Responsive Nanoparticles for Smart Drug Delivery: A NIR Light-Sensitive and Redox-Reactive PEG-PCL-Based System.

IF 5.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Biomacromolecules Pub Date : 2024-11-11 DOI:10.1021/acs.biomac.4c00889

Nancy Ferrentino, Taha Behroozi Kohlan, Shokoufeh Mehrtashfar, Anna Finne-Wistrand, Daniela Pappalardo

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Abstract

Stimuli-responsive polymeric nanoparticles (NPs) can serve as smart drug delivery systems (DDSs) by triggering drug release upon external or internal stimuli. A dual-responsive DDS made of a triblock poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-SS-PEG-SS-PCL) copolymer, bearing disulfide bonds between PCL and PEG, was synthesized. The copolymer was functionalized with coumarin and sensitive to near-infrared (NIR) light irradiation, while the S-S bonds could be cleaved by GSH (10 mM). Characterization was achieved by nuclear magnetic resonance, size exclusion chromatography, and Fourier transform infrared analyses. Nile Red (NR)-loaded NPs were prepared through self-assembly of the copolymer in water and analyzed by dynamic light scattering and field-emission scanning electron microscopy. The NR release upon ultraviolet (UV)/NIR light irradiation as well as by GSH concentrations was monitored by using fluorescence spectroscopy, while simultaneous exposure to UV/NIR light and intracellular GSH concentration led to faster NR release. AlamarBlue assay showed satisfactory cell viability of the NR-loaded NPs, while their cellular uptake in human dermal fibroblast cells was investigated by fluorescence microscopy and fluorescence emission measurements.

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用于智能给药的双响应纳米颗粒：基于近红外光敏和氧化还原反应的 PEG-PCL 系统。

刺激响应型聚合物纳米粒子（NPs）可作为智能给药系统（DDSs），在外部或内部刺激下触发药物释放。本研究合成了一种由三嵌段聚（ε-己内酯）-聚（乙二醇）-聚（ε-己内酯）（PCL-SS-PEG-SS-PCL）共聚物组成的双响应 DDS，PCL 和 PEG 之间存在二硫键。该共聚物被香豆素官能化，对近红外（NIR）光照射敏感，S-S 键可被 GSH（10 mM）裂解。通过核磁共振、尺寸排阻色谱和傅立叶变换红外分析对其进行了表征。通过共聚物在水中的自组装制备了负载尼罗红（NR）的 NPs，并通过动态光散射和场发射扫描电子显微镜进行了分析。荧光光谱法监测了紫外线/近红外光照射以及 GSH 浓度对 NR 释放的影响，而同时照射紫外线/近红外光和细胞内 GSH 浓度会导致 NR 释放更快。AlamarBlue 分析表明负载 NR 的 NPs 具有令人满意的细胞活力，而荧光显微镜和荧光发射测量则研究了 NPs 在人真皮成纤维细胞中的细胞吸收情况。

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来源期刊

Biomacromolecules 化学-高分子科学

CiteScore

10.60

自引率

4.80%

发文量

417

审稿时长

1.6 months

期刊介绍： Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.