Distinct O-Acetylation Patterns of Serum Glycoproteins among Humans, Mice, and Rats.

IF 3.8 2区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS Journal of Proteome Research Pub Date : 2024-12-06 Epub Date: 2024-11-12 DOI:10.1021/acs.jproteome.4c00653
Didi Liu, Yue Xue, Dan Ding, Bojing Zhu, Jiechen Shen, Zhehui Jin, Shisheng Sun
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Abstract

O-Acetylation is a significant chemical modification of sialic acids on glycoproteins with diverse biological functions. As two important animal models, mice and rats have been widely used for various biomedical studies. In this study, we show that the sialic acid types and their O-acetylation patterns have large differences among serum glycoproteins of humans, rats, and mice. Based on intact N-glycopeptide analyses, all sialoglycopeptides in human sera were modified by Neu5Ac without any O-acetylation; 90% of sialoglycopeptides in rat sera were also modified by Neu5Ac, with more than 60% that were further O-acetylated. In contrast, 99% of sialoglycopeptides in mouse sera contained Neu5Gc including 12% in O-acetylated forms. Among all O-acetylated N-glycans, rat sera had hybrid glycans fivefold those of mouse sera, while mouse sera contained 5.5-fold core-fucosylated glycans and 4.6-31.5-fold mono-/penta-/hexa-antenna glycans compared to mice. The overall O-acetylation proportions of serum glycoproteins in rats were much higher than those in mice, and diverse O-acetylation proportions also commonly existed at different glycosites of the same glycoproteins. This study enhances our understanding of O-acetylated sialoglycan diversities and underscores the necessity of considering glycosylation profiles when selecting suitable animal models for various biomedical studies.

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人类、小鼠和大鼠血清糖蛋白不同的 O-乙酰化模式
O-乙酰化是糖蛋白上硅酸的一种重要化学修饰,具有多种生物学功能。作为两种重要的动物模型,小鼠和大鼠已被广泛用于各种生物医学研究。在这项研究中,我们发现人、大鼠和小鼠血清糖蛋白中的硅酸类型及其 O-乙酰化模式存在很大差异。根据完整的 N-糖肽分析,人类血清中所有的硅糖肽都被 Neu5Ac 修饰,没有任何 O-乙酰化;大鼠血清中 90% 的硅糖肽也被 Neu5Ac 修饰,其中超过 60% 的硅糖肽被进一步 O-乙酰化。相比之下,小鼠血清中 99% 的ialoglycopeptides 含有 Neu5Gc,其中 12% 为 O-乙酰化形式。在所有 O-乙酰化的 N-聚糖中,大鼠血清中的杂交聚糖是小鼠血清的 5 倍,而小鼠血清中的核心-岩藻糖基化聚糖是大鼠血清的 5.5 倍,单/五/六触角聚糖是小鼠血清的 4.6-31.5 倍。大鼠血清糖蛋白的总体 O-乙酰化比例远高于小鼠,而且同一糖蛋白的不同聚糖也普遍存在不同的 O-乙酰化比例。这项研究加深了我们对 O-乙酰化硅聚糖多样性的理解,并强调了在选择合适的动物模型进行各种生物医学研究时考虑糖基化特征的必要性。
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来源期刊
Journal of Proteome Research
Journal of Proteome Research 生物-生化研究方法
CiteScore
9.00
自引率
4.50%
发文量
251
审稿时长
3 months
期刊介绍: Journal of Proteome Research publishes content encompassing all aspects of global protein analysis and function, including the dynamic aspects of genomics, spatio-temporal proteomics, metabonomics and metabolomics, clinical and agricultural proteomics, as well as advances in methodology including bioinformatics. The theme and emphasis is on a multidisciplinary approach to the life sciences through the synergy between the different types of "omics".
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