Therapeutic Dose of Zinc Aspartate and Zinc Citrate Attenuates Disease Activity Indices in Rheumatoid Arthritis.

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biological Trace Element Research Pub Date : 2024-11-13 DOI:10.1007/s12011-024-04439-3
Mohammad Hasan, Pooja Yadav, Mairaj Ahmed Ansari, Shakir Ali, Haider A Khan
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Abstract

Zinc aspartate and zinc citrate have been used as zinc supplements in different health conditions. Taking into consideration their anti-inflammatory, immunomodulatory, anti-oxidant and antimicrobial properties, the present study has been designed to analyse the effect of zinc aspartate and zinc citrate treatment at therapeutic dose level on disease severity index, haematological, serological, antimicrobial and radiological markers of rheumatoid arthritis in Wistar rats. Bactericidal potential of the two organic zinc compounds was analysed in vitro in clinically isolated Escherichia coli. Arthritis was induced in male Wistar rats by intradermal injection of an emulsion containing collagen type II and Complete Freund's Adjuvant (CFA) containing 1 mg mL-1 Mycobacterium tuberculosis H37Ra. Zinc aspartate and zinc citrate were orally administered after the onset of the disease for 4 weeks. Ameliorative effect of zinc aspartate and zinc citrate was evaluated by analysing indices of severity and disease activity markers of rheumatoid arthritis. The liver and kidney function tests were performed to evaluate any possible adverse effect of compounds. Antimicrobial activity of the zinc compounds was assessed in clinically isolated E. coli by MTT assay. Zinc aspartate and zinc citrate equivalent to a therapeutic dose of 50 mg/day of elemental zinc attenuated the clinical characteristic of rheumatoid arthritis in the animal model of arthritis, collagen-induced arthritis (CIA). Both zinc salts also exhibited antimicrobial effects against E. coli. The selected dose of zinc aspartate and zinc citrate showed no adverse effects in treated rats. This study highlights the potentiality of zinc compounds as antiarthritic agents and also point to its preventive effects on microbial growth that has been observed in rheumatoid arthritis patients due to their increased sensitivity for bacterial infection.

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治疗剂量的天门冬氨酸锌和柠檬酸锌可减轻类风湿关节炎的疾病活动指数。
天门冬氨酸锌和柠檬酸锌已被用作不同健康状况下的锌补充剂。考虑到天门冬氨酸锌和柠檬酸锌的抗炎、免疫调节、抗氧化和抗菌特性,本研究旨在分析治疗剂量水平的天门冬氨酸锌和柠檬酸锌对 Wistar 大鼠类风湿性关节炎的疾病严重程度指数、血液学、血清学、抗微生物学和放射学指标的影响。体外分析了两种有机锌化合物对临床分离的大肠杆菌的杀菌潜力。通过皮内注射含有 II 型胶原蛋白和含有 1 mg mL-1 结核分枝杆菌 H37Ra 的完全弗氏佐剂(CFA)乳液,诱导雄性 Wistar 大鼠患上关节炎。发病后口服天门冬氨酸锌和柠檬酸锌4周。通过分析类风湿性关节炎的严重程度指数和疾病活动指标,评估了天门冬氨酸锌和柠檬酸锌的改善效果。此外,还进行了肝肾功能检测,以评估化合物可能产生的不良影响。通过 MTT 试验评估了锌化合物在临床分离的大肠杆菌中的抗菌活性。天门冬氨酸锌和柠檬酸锌相当于 50 毫克/天的锌元素治疗剂量,可减轻类风湿关节炎动物模型--胶原诱发关节炎(CIA)的临床特征。这两种锌盐还对大肠杆菌有抗菌作用。所选剂量的天门冬氨酸锌和柠檬酸锌对大鼠没有不良影响。这项研究强调了锌化合物作为抗关节炎药物的潜力,同时也指出了锌化合物对微生物生长的预防作用。
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来源期刊
Biological Trace Element Research
Biological Trace Element Research 生物-内分泌学与代谢
CiteScore
8.70
自引率
10.30%
发文量
459
审稿时长
2 months
期刊介绍: Biological Trace Element Research provides a much-needed central forum for the emergent, interdisciplinary field of research on the biological, environmental, and biomedical roles of trace elements. Rather than confine itself to biochemistry, the journal emphasizes the integrative aspects of trace metal research in all appropriate fields, publishing human and animal nutritional studies devoted to the fundamental chemistry and biochemistry at issue as well as to the elucidation of the relevant aspects of preventive medicine, epidemiology, clinical chemistry, agriculture, endocrinology, animal science, pharmacology, microbiology, toxicology, virology, marine biology, sensory physiology, developmental biology, and related fields.
期刊最新文献
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