Growth response of Vibrio cholerae O1 and V. cholerae non O1/non O139 strains to algae extracts from stream water in far north Cameroon.

IF 1.3 Q4 MICROBIOLOGY Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI:10.18502/ijm.v16i5.16796

Moussa Djaouda, Roméo Wakayansam Bouba, Pierre Nestor Nguimbous, Pagoui Ehbiakbo, Eric Moïse Bakwo Fils, Céline Nguefeu Nkenfou

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Abstract

Background and objectives: Vibrio cholerae O1 or non O1/non O139 is found in water ecosystems where it colonizes phytoplankton and has different lifestyle. This study aimed to investigate the impact of some algae extracts on the survival/growth of both V. cholerae strains.

Materials and methods: Algae extracts consisting of three fractions, F1 containing chlorophyll-a, F2 containing chlorophyll-b, and F3 containing carotenoids, and raw extract (RAE) were obtained from the algal bloom collected in the Kaliao stream (Maroua, Cameroon). The survival and growth of V. cholerae O1 and V. cholerae non O1/non O139, in microcosms consisting of sterile saline with these extracts and peptone (PEP) respectively added at concentrations of 0.01, 0.05 and 0.1 mg/L, and 50/50 mixtures F1+F2, F2+F3, and F2+PEP at a concentration 0.05 mg/L, were compared during a 24h experiment.

Results: The microcosms F2 and RAE did not support the growth of O1 strain; V. cholerae non O1/non O139 count in all algae extract microcosms ranging from 3.97 log (CFU/mL) to 5.2 log (CFU/mL). In all PEP microcosms, the counts of both strains reached an uncountable value. Microcosms F1+F2 and F2+F3 supported the growth of V. cholerae O1 and V. cholerae non-O1/nonO139 strains.

Conclusion: The algae compounds showed strain-specific effect on the growth of V. cholerae.

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霍乱弧菌O1和霍乱弧菌非O1/非O139菌株对喀麦隆最北部溪水中藻类提取物的生长反应。

背景和目的：霍乱弧菌O1或非O1/非O139存在于水生态系统中，它在浮游植物中定植，具有不同的生活方式。本研究旨在调查一些藻类提取物对两种霍乱弧菌菌株存活/生长的影响：藻类提取物包括三个部分：含叶绿素-a的F1、含叶绿素-b的F2和含类胡萝卜素的F3，以及原提取物（RAE）。在 24 小时的实验中，比较了霍乱弧菌 O1 和霍乱弧菌非 O1/ 非 O139 在无菌生理盐水、分别添加浓度为 0.01、0.05 和 0.1 毫克/升的这些提取物和蛋白胨（PEP）以及浓度为 0.05 毫克/升的 F1+F2、F2+F3 和 F2+PEP 的 50/50 混合物组成的微生态系统中的存活和生长情况：F2和RAE微生态环境不支持O1菌株的生长；在所有海藻提取物微生态环境中，非O1/非O139霍乱弧菌的数量从3.97 log (CFU/mL)到5.2 log (CFU/mL)不等。在所有 PEP 微培养液中，两种菌株的计数都达到了无法计数的值。F1+F2 和 F2+F3 微生物培养液支持霍乱弧菌 O1 和霍乱弧菌非 O1/ 非 O139 菌株的生长：结论：藻类复合物对霍乱弧菌的生长具有菌株特异性影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.