Harnessing Biocontrol Potential of Streptomyces rochei Against Pythium aphanidermatum: Efficacy and Mechanisms.

IF 3.5 4区生物学 Q2 MICROBIOLOGY Journal of Basic Microbiology Pub Date : 2024-11-10 DOI:10.1002/jobm.202400531

Iruthayasamy Johnson, Rangasamy Kavitha, Muthusamy Karthikeyan, Mookkan Paramasivan, Dhanabalan Shanmuga Priya, Rangasamy Anandham, Sevugapperumal Nakkeeran

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Abstract

Tomato (Solanum lycopersicum) and chilli (Capsicum annuum) are globally significant vegetable crops susceptible to damping-off disease caused by Pythium aphanidermatum, leading to substantial yield losses. The study aimed to document the biocontrol and plant growth promotion potential of Streptomyces rochei against damping-off disease in tomato and chilli. The actinobacterial isolates ACS18 followed by ACT30, and AOE12 were accomplished as the most effective antagonists against P. aphanidermatum in vitro. Molecular characterization confirmed these isolates as members of Streptomyces genus, with ASH 18 the top performer identified as S. rochei isolate. Analysis of biomolecule through GC-MS during ditrophic interaction between pathogen and S. rochei showed the presence of various antifungal metabolites which were directly related to suppression of the pathogen. Subsequently, S. rochei was formulated into a talc-based preparation and used as seed treatment and soil application against damping-off. In greenhouse trials, significant reductions in damping-off incidence were observed, Furthermore, seedlings treated with S. rochei displayed enhanced root and shoot lengths compared to the uninoculated controls. These benefits potentiate S. rochei as a promising biocontrol agent and demonstrating its dual benefits of disease suppression and promotion of seedling growth.

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利用 Streptomyces rochei 对 Pythium aphanidermatum 的生物防治潜力：功效与机制。

番茄（Solanum lycopersicum）和辣椒（Capsicum annuum）是全球重要的蔬菜作物，容易感染由蚜虫（Pythium aphanidermatum）引起的潮湿病，导致大量减产。本研究旨在记录链霉菌对番茄和辣椒受潮病的生物防治和植物生长促进潜力。在体外实验中，放线菌分离物 ACS18、ACT30 和 AOE12 是对 P. aphanidermatum 最有效的拮抗剂。分子鉴定证实这些分离物属于链霉菌属，其中表现最好的 ASH 18 被鉴定为 S. rochei 分离物。通过 GC-MS 分析病原体与 S. rochei 的双营养相互作用过程中的生物大分子，发现存在多种抗真菌代谢物，这些代谢物与抑制病原体直接相关。随后，S. rochei 被配制成以滑石粉为基础的制剂，用于种子处理和土壤施用，以防治潮湿病。此外，与未接种的对照组相比，用 S. rochei 处理过的幼苗根长和芽长均有所提高。这些益处增强了 S. rochei 作为生物防治剂的潜力，并显示出其抑制病害和促进秧苗生长的双重功效。

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来源期刊

Journal of Basic Microbiology 生物-微生物学

CiteScore

6.10

自引率

0.00%

发文量

134

审稿时长

1.8 months

期刊介绍： The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions. Papers published deal with: microbial interactions (pathogenic, mutualistic, environmental), ecology, physiology, genetics and cell biology/development, new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications) novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).