Determination of Photosensitizing Potential of Lapachol for Photodynamic Inactivation of Bacteria.

IF 4.2 2区化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecules Pub Date : 2024-11-02 DOI:10.3390/molecules29215184

Regiane G Lima, Raphael S Flores, Gabriella Miessi, Jhoenne H V Pulcherio, Laís F Aguilera, Leandro O Araujo, Samuel L Oliveira, Anderson R L Caires

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Abstract

Antimicrobial photodynamic inactivation (aPDI) offers a promising alternative to combat drug-resistant bacteria. This study explores the potential of lapachol, a natural naphthoquinone derived from Tabebuia avellanedae, as a photosensitizer (PS) for aPDI. Lapachol's photosensitizing properties were evaluated using Staphylococcus aureus and Escherichia coli strains under blue LED light (450 nm). UV-vis spectroscopy confirmed lapachol's absorption peak at 482 nm, aligning with effective excitation wavelengths for phototherapy. Photoinactivation assays demonstrated significant bacterial growth inhibition, achieving complete eradication of S. aureus at 25 µg·mL^-1 under light exposure. Scanning electron microscopy (SEM) revealed morphological damage in irradiated bacterial cells, confirming lapachol's bactericidal effect. This research underscores lapachol's potential as a novel photosensitizer in antimicrobial photodynamic therapy, addressing a critical need in combating antibiotic resistance.

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测定拉帕酚在光动力灭活细菌方面的光敏潜力

抗菌光动力灭活（aPDI）为抗击耐药细菌提供了一种前景广阔的替代方法。本研究探索了拉帕酚作为 aPDI 光敏剂（PS）的潜力，拉帕酚是从 Tabebuia avellanedae 中提取的一种天然萘醌。在蓝色 LED 光（450 纳米）下，使用金黄色葡萄球菌和大肠杆菌菌株对拉帕酚的光敏特性进行了评估。紫外可见光谱证实，拉帕酚的吸收峰在 482 纳米处，与光疗的有效激发波长一致。光灭活试验表明，拉帕酚能显著抑制细菌生长，在 25 µg-mL-1 的光照射下，金黄色葡萄球菌可被完全消灭。扫描电子显微镜（SEM）显示了照射后细菌细胞的形态损伤，证实了拉帕酚的杀菌效果。这项研究强调了拉帕酚作为新型光敏剂在抗菌光动力疗法中的潜力，解决了抗生素耐药性的关键需求。

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来源期刊

Molecules 化学-有机化学

CiteScore

7.40

自引率

8.70%

发文量

7524

审稿时长

1.4 months

期刊介绍： Molecules (ISSN 1420-3049, CODEN: MOLEFW) is an open access journal of synthetic organic chemistry and natural product chemistry. All articles are peer-reviewed and published continously upon acceptance. Molecules is published by MDPI, Basel, Switzerland. Our aim is to encourage chemists to publish as much as possible their experimental detail, particularly synthetic procedures and characterization information. There is no restriction on the length of the experimental section. In addition, availability of compound samples is published and considered as important information. Authors are encouraged to register or deposit their chemical samples through the non-profit international organization Molecular Diversity Preservation International (MDPI). Molecules has been launched in 1996 to preserve and exploit molecular diversity of both, chemical information and chemical substances.